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Int. J. Mol. Sci. 2011, 12(12), 9342-9353; doi:10.3390/ijms12129342
Article

The Relationships Between Biological Activities and Structure of Flavan-3-Ols

1
, 2
, 1
, 3,4
 and 1,5,*
Received: 27 October 2011; in revised form: 23 November 2011 / Accepted: 5 December 2011 / Published: 13 December 2011
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Abstract: Flavan-3-ols are involved in multiple metabolic pathways that induce inhibition of cell proliferation. We studied the structure-activity relationship of gallic acid (GA) and four flavan-3-ols: epigallocatechin gallate (EGCG), epigallocatechin (EGC), catechin (C), and epicatechin (EC). We measured the cell viability by the MTT assay and we determined the concentration of testing compound required to reduce cell viability by 50% (IC50). All tested compounds showed a dose-dependent and time-dependent inhibitory antiproliferative effect on Hs578T cells; IC50 values varying from the 15.81 to 326.8 µM. Intracellular ROS (reactive oxygen species) were quantified using a fluorescent probe 2’,7’-dichlorofluorescin diacetate (DCFH-DA). Only the treatment with 10 µM EGC and EGCG was able to induce a significant decrease of ROS concentration and increased levels of ROS were registered for 100 µM EGCG, EGC and GA. Flavans-3-ols and GA induced apoptosis in a dose- and time-dependent manner, which indicated that the induction of apoptosis mediated their cytotoxic activity at least partially. The galloylated catechins have shown a stronger antiproliferative activity and apoptotic effect than the one produced by non galloylated catechins. The galloylated flavan-3-ols are potential therapeutic agents for patients with triple negative breast cancer via induction of apoptosis.
Keywords: catechins; structure-activity relationship; gallate moiety; cell proliferation; apoptosis; pro/antioxidant catechins; structure-activity relationship; gallate moiety; cell proliferation; apoptosis; pro/antioxidant
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Braicu, C.; Pilecki, V.; Balacescu, O.; Irimie, A.; Berindan Neagoe, I. The Relationships Between Biological Activities and Structure of Flavan-3-Ols. Int. J. Mol. Sci. 2011, 12, 9342-9353.

AMA Style

Braicu C, Pilecki V, Balacescu O, Irimie A, Berindan Neagoe I. The Relationships Between Biological Activities and Structure of Flavan-3-Ols. International Journal of Molecular Sciences. 2011; 12(12):9342-9353.

Chicago/Turabian Style

Braicu, Cornelia; Pilecki, Valentina; Balacescu, Ovidiu; Irimie, Alexandru; Berindan Neagoe, Ioana. 2011. "The Relationships Between Biological Activities and Structure of Flavan-3-Ols." Int. J. Mol. Sci. 12, no. 12: 9342-9353.



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