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Int. J. Mol. Sci. 2011, 12(11), 8096-8104; doi:10.3390/ijms12118096
Article

Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes

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1 School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China 2 National Research Center of Soybean Engineering and Technology, Northeast Agriculture University, 201 GongBin Road, XiangFang District, Harbin 150030, China 3 School of Forestry, Northeast Forestry University, 26 HeXing Road, DongLi District, Harbin 150040, China 4 Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115-5737, USA
* Author to whom correspondence should be addressed.
Received: 21 October 2011 / Revised: 8 November 2011 / Accepted: 9 November 2011 / Published: 17 November 2011
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Abstract

The aim of this study was to investigate radioprotective effect of the polysaccharides from soybean meal (SMP) against X-ray radiation-induced damage in mouse spleen lymphocytes. MTT and comet assay were performed to evaluate SMP’s ability to prevent cell death and DNA damage induced by radiation. The results show that, X-ray radiation (30 KV, 10 mA, 8 min (4 Gy)) can significantly increase cell death and DNA fragmentation of mouse spleen lymphocytes. Pretreatment with SMP for 2 h before radiation could increase cell viability, moreover, the SMP can reduce X-ray radiation-induced DNA damage. The percentage of tail DNA and the tail moment of the SMP groups were significantly lower than those of the radiation alone group (p < 0.05). These results suggest SMP may be a good candidate as a radioprotective agent.
Keywords: soybean meal polysaccharides; spleen lymphocytes; radioprotective; comet assay soybean meal polysaccharides; spleen lymphocytes; radioprotective; comet assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yao, L.; Wang, Z.; Zhao, H.; Cheng, C.; Fu, X.; Liu, J.; Yang, X. Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes. Int. J. Mol. Sci. 2011, 12, 8096-8104.

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