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Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes
School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China
National Research Center of Soybean Engineering and Technology, Northeast Agriculture University, 201 GongBin Road, XiangFang District, Harbin 150030, China
School of Forestry, Northeast Forestry University, 26 HeXing Road, DongLi District, Harbin 150040, China
Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115-5737, USA
* Author to whom correspondence should be addressed.
Received: 21 October 2011; in revised form: 8 November 2011 / Accepted: 9 November 2011 / Published: 17 November 2011
Abstract: The aim of this study was to investigate radioprotective effect of the polysaccharides from soybean meal (SMP) against X-ray radiation-induced damage in mouse spleen lymphocytes. MTT and comet assay were performed to evaluate SMP’s ability to prevent cell death and DNA damage induced by radiation. The results show that, X-ray radiation (30 KV, 10 mA, 8 min (4 Gy)) can significantly increase cell death and DNA fragmentation of mouse spleen lymphocytes. Pretreatment with SMP for 2 h before radiation could increase cell viability, moreover, the SMP can reduce X-ray radiation-induced DNA damage. The percentage of tail DNA and the tail moment of the SMP groups were significantly lower than those of the radiation alone group (p < 0.05). These results suggest SMP may be a good candidate as a radioprotective agent.
Keywords: soybean meal polysaccharides; spleen lymphocytes; radioprotective; comet assay
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MDPI and ACS Style
Yao, L.; Wang, Z.; Zhao, H.; Cheng, C.; Fu, X.; Liu, J.; Yang, X. Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes. Int. J. Mol. Sci. 2011, 12, 8096-8104.
Yao L, Wang Z, Zhao H, Cheng C, Fu X, Liu J, Yang X. Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes. International Journal of Molecular Sciences. 2011; 12(11):8096-8104.
Yao, Lei; Wang, Zhenyu; Zhao, Haitian; Cheng, Cuilin; Fu, Xiaoyi; Liu, Jiaren; Yang, Xin. 2011. "Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes." Int. J. Mol. Sci. 12, no. 11: 8096-8104.