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An Efficient Method for Genomic DNA Extraction from Different Molluscs Species
Institute for Biotechnology and Bioengineering, Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), Apdo1013, Vila Real 5001-801, Portugal
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Apdo1013, Vila Real 5001-801, Portugal
National Institute of Biological Resources (INRB, I.P.)/IPIMAR, Avenue 5 de Outubro s/n, Olhão P-8700-305, Portugal
* Author to whom correspondence should be addressed.
Received: 13 September 2011; in revised form: 20 October 2011 / Accepted: 8 November 2011 / Published: 17 November 2011
Abstract: The selection of a DNA extraction method is a critical step when subsequent analysis depends on the DNA quality and quantity. Unlike mammals, for which several capable DNA extraction methods have been developed, for molluscs the availability of optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such as animal physiology, the type (e.g., adductor muscle or gills) or quantity of tissue, can explain the lack of efficiency (quality and yield) in molluscs genomic DNA extraction procedure. In an attempt to overcome these aspects, this work describes an efficient method for molluscs genomic DNA extraction that was tested in several species from different orders: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) and Muricidae (Gastropoda), with different weight sample tissues. The isolated DNA was of high molecular weight with high yield and purity, even with reduced quantities of tissue. Moreover, the genomic DNA isolated, demonstrated to be suitable for several downstream molecular techniques, such as PCR sequencing among others.
Keywords: molluscs; DNA; QuickGene-810; DNA yield; concentration and purity
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Pereira, J.C.; Chaves, R.; Bastos, E.; Leitão, A.; Guedes-Pinto, H. An Efficient Method for Genomic DNA Extraction from Different Molluscs Species. Int. J. Mol. Sci. 2011, 12, 8086-8095.
Pereira JC, Chaves R, Bastos E, Leitão A, Guedes-Pinto H. An Efficient Method for Genomic DNA Extraction from Different Molluscs Species. International Journal of Molecular Sciences. 2011; 12(11):8086-8095.
Pereira, Jorge C.; Chaves, Raquel; Bastos, Estela; Leitão, Alexandra; Guedes-Pinto, Henrique. 2011. "An Efficient Method for Genomic DNA Extraction from Different Molluscs Species." Int. J. Mol. Sci. 12, no. 11: 8086-8095.