Next Article in Journal
Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes
Previous Article in Journal
Chromatin Structure Following UV-Induced DNA Damage—Repair or Death?
Int. J. Mol. Sci. 2011, 12(11), 8086-8095; doi:10.3390/ijms12118086
Technical Note

An Efficient Method for Genomic DNA Extraction from Different Molluscs Species

1,2,* , 1,2
Received: 13 September 2011 / Revised: 20 October 2011 / Accepted: 8 November 2011 / Published: 17 November 2011
View Full-Text   |   Download PDF [551 KB, uploaded 19 June 2014]   |   Browse Figures


The selection of a DNA extraction method is a critical step when subsequent analysis depends on the DNA quality and quantity. Unlike mammals, for which several capable DNA extraction methods have been developed, for molluscs the availability of optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such as animal physiology, the type (e.g., adductor muscle or gills) or quantity of tissue, can explain the lack of efficiency (quality and yield) in molluscs genomic DNA extraction procedure. In an attempt to overcome these aspects, this work describes an efficient method for molluscs genomic DNA extraction that was tested in several species from different orders: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) and Muricidae (Gastropoda), with different weight sample tissues. The isolated DNA was of high molecular weight with high yield and purity, even with reduced quantities of tissue. Moreover, the genomic DNA isolated, demonstrated to be suitable for several downstream molecular techniques, such as PCR sequencing among others.
Keywords: molluscs; DNA; QuickGene-810; DNA yield; concentration and purity molluscs; DNA; QuickGene-810; DNA yield; concentration and purity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Pereira, J.C.; Chaves, R.; Bastos, E.; Leitão, A.; Guedes-Pinto, H. An Efficient Method for Genomic DNA Extraction from Different Molluscs Species. Int. J. Mol. Sci. 2011, 12, 8086-8095.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert