Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species

doi:10.3390/ijms12117835

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Int. J. Mol. Sci. 2011, 12(11), 7835-7845; doi:10.3390/ijms12117835

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Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species

Heng-Sheng Lin, Chih-Yun Chiang, Song-Bin Chang* and Chang-Sheng Kuoh

Department of Life Sciences, Institute of Biodiversity, National Cheng Kung University, Tainan 70101, Taiwan

* Author to whom correspondence should be addressed.

Received: 14 October 2011; in revised form: 28 October 2011 / Accepted: 4 November 2011 / Published: 11 November 2011

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Abstract: Foxtail millet is one of the world’s oldest cultivated crops. It has been adopted as a model organism for providing a deeper understanding of plant biology. In this study, 45 simple sequence repeats (SSR) markers of Setaria italica were developed. These markers showing polymorphism were screened in 223 samples from 12 foxtail millet populations around Taiwan. The most common dinucleotide and trinucleotide repeat motifs are AC/TG (84.21%) and CAT (46.15%). The average number of alleles (N_a), the average heterozygosities observed (H_o) and expected (H_e) are 3.73, 0.714, 0.587, respectively. In addition, 24 SSR markers had shown transferability to six related Poaceae species. These new markers provide tools for examining genetic relatedness among foxtail millet populations and other related species. It is suitable for germplasm management and protection in Poaceae.

Keywords: model system; Setaria italica; SSRs; Taiwan; transferability

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MDPI and ACS Style

Lin, H.-S.; Chiang, C.-Y.; Chang, S.-B.; Kuoh, C.-S. Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species. Int. J. Mol. Sci. 2011, 12, 7835-7845.

AMA Style

Lin H-S, Chiang C-Y, Chang S-B, Kuoh C-S. Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species. International Journal of Molecular Sciences. 2011; 12(11):7835-7845.

Chicago/Turabian Style

Lin, Heng-Sheng; Chiang, Chih-Yun; Chang, Song-Bin; Kuoh, Chang-Sheng. 2011. "Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species." Int. J. Mol. Sci. 12, no. 11: 7835-7845.

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