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Int. J. Mol. Sci. 2011, 12(11), 7835-7845; doi:10.3390/ijms12117835

Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species

Department of Life Sciences, Institute of Biodiversity, National Cheng Kung University, Tainan 70101, Taiwan
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Received: 14 October 2011 / Revised: 28 October 2011 / Accepted: 4 November 2011 / Published: 11 November 2011
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Abstract

Foxtail millet is one of the world’s oldest cultivated crops. It has been adopted as a model organism for providing a deeper understanding of plant biology. In this study, 45 simple sequence repeats (SSR) markers of Setaria italica were developed. These markers showing polymorphism were screened in 223 samples from 12 foxtail millet populations around Taiwan. The most common dinucleotide and trinucleotide repeat motifs are AC/TG (84.21%) and CAT (46.15%). The average number of alleles (Na), the average heterozygosities observed (Ho) and expected (He) are 3.73, 0.714, 0.587, respectively. In addition, 24 SSR markers had shown transferability to six related Poaceae species. These new markers provide tools for examining genetic relatedness among foxtail millet populations and other related species. It is suitable for germplasm management and protection in Poaceae.
Keywords: model system; Setaria italica; SSRs; Taiwan; transferability model system; Setaria italica; SSRs; Taiwan; transferability
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Lin, H.-S.; Chiang, C.-Y.; Chang, S.-B.; Kuoh, C.-S. Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species. Int. J. Mol. Sci. 2011, 12, 7835-7845.

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