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Int. J. Mol. Sci. 2011, 12(11), 7818-7823; doi:10.3390/ijms12117818
Article

Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)

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Received: 8 October 2011 / Revised: 31 October 2011 / Accepted: 1 November 2011 / Published: 11 November 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Fourteen polymorphic microsatellite loci were isolated from the genomic DNA of Pistacia weinmannifolia, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) method, and screened on 12 individuals from each of two wild populations. The 14 polymorphic loci had an average of 4.1 alleles per locus varying from 1 to 9. The observed (Ho) and expected (He) heterozygosities across the two populations ranged from 0.000 to 0.933 and from 0.000 to 0.906, respectively. Tests for departure from Hardy-Weinberg equilibrium (HWE) and genotypic linkage disequilibrium (LD) were conducted for each of the two populations separately. It was found that no locus significantly deviated from HWE proportions and no significant LD was detected between loci (p < 0.001). In the test of cross-species utility, we successfully amplified nine (64.2%) of 14 loci in P. chinensis and four (28.6%) in P. mexicana. The relatively high level of polymorphism for these markers will facilitate further studies of gene flow, population structure and evolutionary history of P. weinmannifolia and its congeners.
Keywords: Pistacia weinmannifolia; microsatellites; Anacardiaceae; genetic structure; polymorphism Pistacia weinmannifolia; microsatellites; Anacardiaceae; genetic structure; polymorphism
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chen, S.; Wu, X.; Ji, Y.; Yang, J. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae). Int. J. Mol. Sci. 2011, 12, 7818-7823.

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