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Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)
Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
School of Life Sciences, Yunnan Normal University, Kunming 650092, China
Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
* Authors to whom correspondence should be addressed.
Received: 8 October 2011; in revised form: 31 October 2011 / Accepted: 1 November 2011 / Published: 11 November 2011
Abstract: Fourteen polymorphic microsatellite loci were isolated from the genomic DNA of Pistacia weinmannifolia, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) method, and screened on 12 individuals from each of two wild populations. The 14 polymorphic loci had an average of 4.1 alleles per locus varying from 1 to 9. The observed (Ho) and expected (He) heterozygosities across the two populations ranged from 0.000 to 0.933 and from 0.000 to 0.906, respectively. Tests for departure from Hardy-Weinberg equilibrium (HWE) and genotypic linkage disequilibrium (LD) were conducted for each of the two populations separately. It was found that no locus significantly deviated from HWE proportions and no significant LD was detected between loci (p < 0.001). In the test of cross-species utility, we successfully amplified nine (64.2%) of 14 loci in P. chinensis and four (28.6%) in P. mexicana. The relatively high level of polymorphism for these markers will facilitate further studies of gene flow, population structure and evolutionary history of P. weinmannifolia and its congeners.
Keywords: Pistacia weinmannifolia; microsatellites; Anacardiaceae; genetic structure; polymorphism
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Chen, S.; Wu, X.; Ji, Y.; Yang, J. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae). Int. J. Mol. Sci. 2011, 12, 7818-7823.
Chen S, Wu X, Ji Y, Yang J. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae). International Journal of Molecular Sciences. 2011; 12(11):7818-7823.
Chen, Shaotian; Wu, Xiayu; Ji, Yunheng; Yang, Junbo. 2011. "Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)." Int. J. Mol. Sci. 12, no. 11: 7818-7823.