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Int. J. Mol. Sci. 2011, 12(11), 7748-7759; doi:10.3390/ijms12117748

A Lateral Flow Protein Microarray for Rapid and Sensitive Antibody Assays

Received: 5 September 2011 / Revised: 31 October 2011 / Accepted: 3 November 2011 / Published: 9 November 2011
(This article belongs to the Special Issue Advances in Molecular Diagnostics)
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Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein microarray device capable of sensitive (< 30 ng/mL) determination of antigen-specific antibodies in ten minutes of total assay time. Results were developed with gold nanobeads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy with an area under curve (AUC of 98% was achieved in comparison with an established glass microarray assay for 26 antigen-specific antibodies. We propose that the presented framework could find use in convenient and cost-efficient quality control of antibody production, as well as in providing a platform for multiplexed affinity-based assays in low-resource or mobile settings.
Keywords: point of care; protein microarray; lateral flow point of care; protein microarray; lateral flow
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gantelius, J.; Bass, T.; Sjöberg, R.; Nilsson, P.; Andersson-Svahn, H. A Lateral Flow Protein Microarray for Rapid and Sensitive Antibody Assays. Int. J. Mol. Sci. 2011, 12, 7748-7759.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert