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Applications of Next-Generation Sequencing Technologies to Diagnostic Virology
Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, I-35121 Padova, Italy
Department of Biological Chemistry, University of Padova, I-35121 Padova, Italy
* Author to whom correspondence should be addressed.
Received: 2 September 2011; in revised form: 20 October 2011 / Accepted: 7 November 2011 / Published: 14 November 2011
Abstract: Novel DNA sequencing techniques, referred to as “next-generation” sequencing (NGS), provide high speed and throughput that can produce an enormous volume of sequences with many possible applications in research and diagnostic settings. In this article, we provide an overview of the many applications of NGS in diagnostic virology. NGS techniques have been used for high-throughput whole viral genome sequencing, such as sequencing of new influenza viruses, for detection of viral genome variability and evolution within the host, such as investigation of human immunodeficiency virus and human hepatitis C virus quasispecies, and monitoring of low-abundance antiviral drug-resistance mutations. NGS techniques have been applied to metagenomics-based strategies for the detection of unexpected disease-associated viruses and for the discovery of novel human viruses, including cancer-related viruses. Finally, the human virome in healthy and disease conditions has been described by NGS-based metagenomics.
Keywords: next generation sequencing; deep sequencing; virus discovery; metagenomics; virome; virology; quasispecies; molecular diagnosis; human immunodeficiency virus; drug resistance; minority variants
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Barzon, L.; Lavezzo, E.; Militello, V.; Toppo, S.; Palù, G. Applications of Next-Generation Sequencing Technologies to Diagnostic Virology. Int. J. Mol. Sci. 2011, 12, 7861-7884.
Barzon L, Lavezzo E, Militello V, Toppo S, Palù G. Applications of Next-Generation Sequencing Technologies to Diagnostic Virology. International Journal of Molecular Sciences. 2011; 12(11):7861-7884.
Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio. 2011. "Applications of Next-Generation Sequencing Technologies to Diagnostic Virology." Int. J. Mol. Sci. 12, no. 11: 7861-7884.