Int. J. Mol. Sci. 2011, 12(11), 7861-7884; doi:10.3390/ijms12117861
Review

Applications of Next-Generation Sequencing Technologies to Diagnostic Virology

1,* email, 1email, 1email, 2email and 1email
Received: 2 September 2011; in revised form: 20 October 2011 / Accepted: 7 November 2011 / Published: 14 November 2011
(This article belongs to the Special Issue Advances in Molecular Diagnostics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Novel DNA sequencing techniques, referred to as “next-generation” sequencing (NGS), provide high speed and throughput that can produce an enormous volume of sequences with many possible applications in research and diagnostic settings. In this article, we provide an overview of the many applications of NGS in diagnostic virology. NGS techniques have been used for high-throughput whole viral genome sequencing, such as sequencing of new influenza viruses, for detection of viral genome variability and evolution within the host, such as investigation of human immunodeficiency virus and human hepatitis C virus quasispecies, and monitoring of low-abundance antiviral drug-resistance mutations. NGS techniques have been applied to metagenomics-based strategies for the detection of unexpected disease-associated viruses and for the discovery of novel human viruses, including cancer-related viruses. Finally, the human virome in healthy and disease conditions has been described by NGS-based metagenomics.
Keywords: next generation sequencing; deep sequencing; virus discovery; metagenomics; virome; virology; quasispecies; molecular diagnosis; human immunodeficiency virus; drug resistance; minority variants
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MDPI and ACS Style

Barzon, L.; Lavezzo, E.; Militello, V.; Toppo, S.; Palù, G. Applications of Next-Generation Sequencing Technologies to Diagnostic Virology. Int. J. Mol. Sci. 2011, 12, 7861-7884.

AMA Style

Barzon L, Lavezzo E, Militello V, Toppo S, Palù G. Applications of Next-Generation Sequencing Technologies to Diagnostic Virology. International Journal of Molecular Sciences. 2011; 12(11):7861-7884.

Chicago/Turabian Style

Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio. 2011. "Applications of Next-Generation Sequencing Technologies to Diagnostic Virology." Int. J. Mol. Sci. 12, no. 11: 7861-7884.

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