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DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA)
Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, P/Bag X1314, Alice 5700, South Africa
Department of Biochemistry and Microbiology, Faculty of Science, University of Buea, Box 63, Buea, Cameroon
* Author to whom correspondence should be addressed.
Received: 22 August 2011; Accepted: 28 October 2011 / Published: 31 October 2011
Abstract: Sequence diversity and population structures can vary widely among pathogenic bacteria species. In some species, all isolates are highly similar, whereas in others most of the isolates are distinguished easily. H. pylori is known for its wide genetic diversity amongst the various strains most especially in the genes involved in virulence. The aim of this study was to evaluate by PCR and sequence analysis, the genetic profile of H. pylori vacA gene (s1, s2, m1 and m2). We sequenced small DNA segments from 13 vacAs1, 10 vacAm2, 6 vacAm1 and 6 vacAs2 strains which were amplified with amplicon size of 259/286 bp, 290 bp and 352 bp for vacAs1/s2, m1 and m2 respectively. Based on similarities among our strains accession numbers were provided for seven vacAs1 (HQ709109–HQ709115), six vacAs2 (JN848463–JN848468), six vacAm1 (JN848469–JN848474) and six vacAm2 (HQ650801–HQ650806) strains. Amongst the strains studied, 98.07%, 98.58%, 97.38% and 95.41% of vacAs1, vacAs2, vacAm1 and vacAm2 of the strains were conserved respectively. Findings of this study underscores the importance of understanding the virulence composition and diversity of H. pylori in South Africa for enhanced clinico-epidemiological monitoring and pathophysiology of disease.
Keywords: diversity; Helicobacter pylori; vacuolating cytotoxin gene (vacA); South Africa
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Tanih, N.F.; Ndip, L.M.; Ndip, R.N. DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA). Int. J. Mol. Sci. 2011, 12, 7459-7468.
Tanih NF, Ndip LM, Ndip RN. DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA). International Journal of Molecular Sciences. 2011; 12(11):7459-7468.
Tanih, Nicoline F.; Ndip, Lucy M.; Ndip, Roland N. 2011. "DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA)." Int. J. Mol. Sci. 12, no. 11: 7459-7468.