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Int. J. Mol. Sci. 2011, 12(10), 7216-7237; doi:10.3390/ijms12107216
Article

Optimal Production and Biochemical Properties of a Lipase from Candida albicans

1,†
, 2,†, 2
, 3
, 2,*  and 4,*
Received: 7 September 2011; in revised form: 12 October 2011 / Accepted: 17 October 2011 / Published: 24 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Lipases from microorganisms have multi-faceted properties and play an important role in ever-growing modern biotechnology and, consequently, it is of great significance to develop new ones. In the present work, a lipase gene from Candida albicans (CaLIP10) was cloned and two non-unusual CUG serine codons were mutated into universal codons, and its expression in Pichia pastoris performed optimally, as shown by response surface methodology. Optimal conditions were: initial pH of culture 6.86, temperature 25.53 °C, 3.48% of glucose and 1.32% of yeast extract. The corresponding maximal lipolytic activity of CaLIP10 was 8.06 U/mL. The purified CaLIP10 showed maximal activity at pH 8.0 and 25 °C, and a good resistance to non-ionic surfactants and polar organic solvent was noticed. CaLIP10 could effectively hydrolyze coconut oil, but exhibited no obvious preference to the fatty acids with different carbon length, and diacylglycerol was accumulated in the reaction products, suggesting that CaLIP10 is a potential lipase for the oil industry.
Keywords: Candida albicans lipases; response surface methodology; biochemical characterization; diacylglycerol; coconut oil Candida albicans lipases; response surface methodology; biochemical characterization; diacylglycerol; coconut oil
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lan, D.; Hou, S.; Yang, N.; Whiteley, C.; Yang, B.; Wang, Y. Optimal Production and Biochemical Properties of a Lipase from Candida albicans. Int. J. Mol. Sci. 2011, 12, 7216-7237.

AMA Style

Lan D, Hou S, Yang N, Whiteley C, Yang B, Wang Y. Optimal Production and Biochemical Properties of a Lipase from Candida albicans. International Journal of Molecular Sciences. 2011; 12(10):7216-7237.

Chicago/Turabian Style

Lan, Dongming; Hou, Shulin; Yang, Ning; Whiteley, Chris; Yang, Bo; Wang, Yonghua. 2011. "Optimal Production and Biochemical Properties of a Lipase from Candida albicans." Int. J. Mol. Sci. 12, no. 10: 7216-7237.



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