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• Lan, D
• Hou, S
• Yang, N
• Whiteley, C
• Yang, B
• Wang, Y
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• Lan, D
• Hou, S
• Yang, N
• Whiteley, C
• Yang, B
• Wang, Y
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• Lan, D
• Hou, S
• Yang, N
• Whiteley, C
• Yang, B
• Wang, Y

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Int. J. Mol. Sci. 2011, 12(10), 7216-7237; doi:10.3390/ijms12107216

Article

Optimal Production and Biochemical Properties of a Lipase from Candida albicans

Dongming Lan ^1,† , Shulin Hou ^2,† , Ning Yang ² , Chris Whiteley ³ , Bo Yang ^2,*  and Yonghua Wang ^4,*

¹ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China ² School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China ³ Department of Biochemistry, Microbiology & Biotechnology, Rhodes University, Grahamstown 6139, South Africa ⁴ Key Lab of Fermentation and Enzyme Engineering, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China ^† These authors contributed equally to this work.

* Authors to whom correspondence should be addressed.

Received: 7 September 2011; in revised form: 12 October 2011 / Accepted: 17 October 2011 / Published: 24 October 2011

(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)

Download PDF Full-Text [928 KB, uploaded 24 October 2011 14:03 CEST]

Abstract: Lipases from microorganisms have multi-faceted properties and play an important role in ever-growing modern biotechnology and, consequently, it is of great significance to develop new ones. In the present work, a lipase gene from Candida albicans (CaLIP10) was cloned and two non-unusual CUG serine codons were mutated into universal codons, and its expression in Pichia pastoris performed optimally, as shown by response surface methodology. Optimal conditions were: initial pH of culture 6.86, temperature 25.53 °C, 3.48% of glucose and 1.32% of yeast extract. The corresponding maximal lipolytic activity of CaLIP10 was 8.06 U/mL. The purified CaLIP10 showed maximal activity at pH 8.0 and 25 °C, and a good resistance to non-ionic surfactants and polar organic solvent was noticed. CaLIP10 could effectively hydrolyze coconut oil, but exhibited no obvious preference to the fatty acids with different carbon length, and diacylglycerol was accumulated in the reaction products, suggesting that CaLIP10 is a potential lipase for the oil industry.

Keywords: Candida albicans lipases; response surface methodology; biochemical characterization; diacylglycerol; coconut oil

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