Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.

doi:10.3390/ijms12010857

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Int. J. Mol. Sci. 2011, 12(1), 857-864; doi:10.3390/ijms12010857

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Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.

Da-Wei Wang, Yu Li and Zhou-Qi Li*

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China

* Author to whom correspondence should be addressed.

Received: 1 January 2011; in revised form: 10 January 2011 / Accepted: 11 January 2011 / Published: 24 January 2011

(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)

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Abstract: Eucommia ulmoides Oliv. is a dioecious species. Currently, there is no method to identify the sex during the juvenile stage that lasts a relatively long time. This study aimed to develop a sex specific Amplified Fragment Length Polymorphism (AFLP) marker for Eucommia ulmoides Oliv. Of a total of 64 AFLP primer combinations screened, primer combination E-ACA/M-CTT produced a 350 bp male-specific marker. This 350 bp AFLP marker was converted into a 247 bp Sequence Characterized Amplified Region (SCAR) marker. Results suggest that the SCAR marker can be utilized for early sexual identification in Eucommia ulmoides Oliv., and it will greatly facilitate future breeding programs.

Keywords: AFLP; SCAR; male-specific; Eucommia ulmoides Oliv.

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MDPI and ACS Style

Wang, D.-W.; Li, Y.; Li, Z.-Q. Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.. Int. J. Mol. Sci. 2011, 12, 857-864.

AMA Style

Wang D-W, Li Y, Li Z-Q. Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.. International Journal of Molecular Sciences. 2011; 12(1):857-864.

Chicago/Turabian Style

Wang, Da-Wei; Li, Yu; Li, Zhou-Qi. 2011. "Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.." Int. J. Mol. Sci. 12, no. 1: 857-864.

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