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Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora
Chair of Histology and Embryology, Institute of Biostructural Research, Medical University of Warsaw, Chalubinskiego 5 Str., 02-004 Warszawa, Poland
Department of Conservative Dentistry, Institute of Stomatology, Medical University of Warsaw, Miodowa 18 Str., 00-246 Warszawa, Poland
* Author to whom correspondence should be addressed.
Received: 14 July 2010; in revised form: 23 August 2010 / Accepted: 6 September 2010 / Published: 15 September 2010
Abstract: HCl-demineralized murine lower incisors were implanted intramuscularly into syngeneic BALB/c mice to induce heterotopic osteogenesis. Implants were exposed at the early, preosteogenic stage (4), or at the later, osteogenic stage (12) to the Moloney sarcoma virus (MSV), which within 3–4 days results in a sarcoma. The yield of bone induction was determined by weight of dry bone mass following NaOH hydrolysis of soft tissues. To verify the effect of this sarcoma on orthotopic local femoral bone, the dry mass of the tumor-exposed femora was measured and compared with the weight of MSV-unexposed contralateral controls. MSV-sarcoma or cells involved with their spontaneous rejection have a stimulatory effect on the periosteal membrane of the tumor-adjacent femoral bones, increasing their dry mass on average by 18%. No stimulatory effect on heterotopic bone induction was observed when the MSV sarcoma grew during the early, preosteogenic stage (4 onward), but when the tooth matrix had been exposed to such tumor at the already bone-forming stage, (12 onward), the yield of bone induction was enhanced. Thus, it is postulated that lesions induced by MSV during the early, preosteogenic stage inhibit recruitment of osteoprogenitor cells or degrade Bone Morphogenetic Proteins (BMPs) released by matrix resorbing inflammatory cells, whereas when acting on already existing bone they have a stimulatory effect.
Keywords: ectopic osteogenesis; murine demineralized incisors; MSV sarcoma; periosteum activation
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Włodarski, K.; Włodarski, P.; Galus, R.; Brodzikowska, A. Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora. Int. J. Mol. Sci. 2010, 11, 3277-3287.
Włodarski K, Włodarski P, Galus R, Brodzikowska A. Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora. International Journal of Molecular Sciences. 2010; 11(9):3277-3287.
Włodarski, Krzysztof; Włodarski, Paweł; Galus, Ryszard; Brodzikowska, Aniela. 2010. "Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora." Int. J. Mol. Sci. 11, no. 9: 3277-3287.