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Int. J. Mol. Sci. 2010, 11(8), 2780-2790; doi:10.3390/ijms11082780
Article

The Effect of a Brief Salivary α-Amylase Exposure During Chewing on Subsequent in Vitro Starch Digestion Curve Profiles

1,2,3
, 1
, 2
 and 3,4,*
1 Food Futures National Research Flagship and CSIRO Food and Nutritional Sciences, Adelaide, Australia 2 New Zealand Institute for Plant & Food Research Limited, Palmerston North, New Zealand 3 Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand 4 Department of Food & Tourism Management, Manchester Metropolitan University, Manchester, UK
* Author to whom correspondence should be addressed.
Received: 22 June 2010 / Revised: 19 July 2010 / Accepted: 19 July 2010 / Published: 26 July 2010
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

There is inconsistency between current in vitro digestion methods with regard to accommodation of a (salivary) α-amylase exposure during the oral phase. The effect of a salivary α-amylase pre-exposure on subsequent in vitro starch digestion curve profiles for various foods was investigated. Foods were chewed, expectorated and the boluses left to rest for 0–15 min. During pancreatic digestion, aliquots were taken and hydrolysis curves constructed for comparison against those of the same foods comminuted with a manually-operated chopper, hence spared exposure to saliva. Hydrolysate aliquots taken at T0 (time zero) of the digestion of chewed samples contained higher levels of glucose and dextrins compared with chopped samples. Pancreatin activity immediately overwhelmed differences in sugar released due to salivary amylase activity. Within 10 min no differences were detectable between hydrolysis curves for chewed and chopped foods. Salivary amylase pretreatment does not contribute to the robustness or relative accuracy of in vitro methods.
Keywords: digestion; in vitro; saliva; starch digestion; in vitro; saliva; starch
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Woolnough, J.W.; Bird, A.R.; Monro, J.A.; Brennan, C.S. The Effect of a Brief Salivary α-Amylase Exposure During Chewing on Subsequent in Vitro Starch Digestion Curve Profiles. Int. J. Mol. Sci. 2010, 11, 2780-2790.

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