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Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris
College of Life Sciences, Fujian Normal University, The University Town, Min-hou, Fuzhou 350108, China
Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350108, China
* Author to whom correspondence should be addressed.
Received: 8 April 2010; in revised form: 10 May 2010 / Accepted: 10 May 2010 / Published: 3 June 2010
Abstract: A lipase gene (atl) was cloned from Aspergillustamarii FS132 for the first time. The gene was found to have an open reading frame of 1024 base pairs (bp), and the coding region of the gene contained two introns (51 bp and 52 bp). Multi-alignment analysis of the deduced amino acid sequence indicated high homology between the enzyme and mono- and diacylglycerol lipases from fungi Aspergillus. The recombinant lipase was expressed in Pichia pastoris GS115 cells. The recombinant lipase was found to have a molecular mass of 36.7 kDa, and it exhibited lipase activity of 20 U/mL in culture supernatant when tributyrin was used as the substrate.
Keywords: Aspergillus tamarii; lipase; gene cloning; expression; Pichia pastoris
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Shi, B.; Zeng, L.; Song, H.; Shi, Q.; Wu, S. Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris . Int. J. Mol. Sci. 2010, 11, 2373-2382.
Shi B, Zeng L, Song H, Shi Q, Wu S. Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris . International Journal of Molecular Sciences. 2010; 11(6):2373-2382.
Shi, Bihong; Zeng, Liqing; Song, Haolei; Shi, Qiaoqin; Wu, Songgang. 2010. "Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris ." Int. J. Mol. Sci. 11, no. 6: 2373-2382.