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Int. J. Mol. Sci. 2010, 11(12), 5323-5338; doi:10.3390/ijms11125323
Article

Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle

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Received: 19 November 2010; in revised form: 20 December 2010 / Accepted: 21 December 2010 / Published: 22 December 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this study, we determined the effects of cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase regulation in three human breast cancer cell lines (T47D, SKBR-3, and MCF-7) and a mammary epithelium cell line (HBL-100). Cell viability after treatment with cucurbitacin B, which is an active ingredient of this herb, was assessed. Telomeric Repeat Amplification Protocol (TRAP) assays and RT-PCR (qualitative and realtime) were performed to investigate activity of telomerase as well as expression of human telomerase reverse transcriptase (hTERT) and c-Myc. The c-Myc protein level was also determined in SKBR-3 and HBL-100 cells. Our results show that the cucurbitacin B inhibits growth and telomerase activity in the three breast cancer cell lines and exerts an obvious inhibitory effect in the estrogen receptor (ER)-negative breast cancer SKBR-3 cells. The expression of hTERT and c-Myc were also inhibited by cucurbitacin B, In addition, a clear reduction of c-Myc protein was observed after treatment in SKBR-3 cells even with a concentration of cucurbitacin B that was ten-times lower compared to the concentration used for HBL-100. Our findings imply that cucurbitacin B exerts an anticancer effect by inhibiting telomerase via down regulating both the hTERT and c-Myc expression in breast cancer cells.
Keywords: cucurbitacin B; telomerase; hTERT; c-Myc; breast cancer; estrogen receptor cucurbitacin B; telomerase; hTERT; c-Myc; breast cancer; estrogen receptor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Duangmano, S.; Dakeng, S.; Jiratchariyakul, W.; Suksamrarn, A.; Smith, D.R.; Patmasiriwat, P. Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle. Int. J. Mol. Sci. 2010, 11, 5323-5338.

AMA Style

Duangmano S, Dakeng S, Jiratchariyakul W, Suksamrarn A, Smith DR, Patmasiriwat P. Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle. International Journal of Molecular Sciences. 2010; 11(12):5323-5338.

Chicago/Turabian Style

Duangmano, Suwit; Dakeng, Sumana; Jiratchariyakul, Weena; Suksamrarn, Apichart; Smith, Duncan R.; Patmasiriwat, Pimpicha. 2010. "Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle." Int. J. Mol. Sci. 11, no. 12: 5323-5338.


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