Abstract: : Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.
Keywords: Fe-chlorophyllin; nitric oxide; IAA oxidase; SOD; POD
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Tong, M.; Zhang, L.; Wang, Y.; Jiang, H.; Ren, Y. Fe-Chlorophyllin Promotes the Growth of Wheat Roots Associated with Nitric Oxide Generation. Int. J. Mol. Sci. 2010, 11, 5246-5255.
Tong M, Zhang L, Wang Y, Jiang H, Ren Y. Fe-Chlorophyllin Promotes the Growth of Wheat Roots Associated with Nitric Oxide Generation. International Journal of Molecular Sciences. 2010; 11(12):5246-5255.
Tong, Min; Zhang, Liefeng; Wang, Yifan; Jiang, Hui; Ren, Yong. 2010. "Fe-Chlorophyllin Promotes the Growth of Wheat Roots Associated with Nitric Oxide Generation." Int. J. Mol. Sci. 11, no. 12: 5246-5255.