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Int. J. Mol. Sci. 2010, 11(12), 5129-5142; doi:10.3390/ijms11125129
Article

Molecular Interactions and Protein-Induced DNA Hairpin in the Transcriptional Control of Bacteriophage Ø29 DNA

*  and
Received: 8 October 2010; in revised form: 22 November 2010 / Accepted: 7 December 2010 / Published: 13 December 2010
(This article belongs to the Special Issue Advances in Molecular Recognition)
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Abstract: Studies on the regulation of phage Ø29 gene expression revealed a new mechanism to accomplish simultaneous activation and repression of transcription leading to orderly gene expression. Two phage-encoded early proteins, p4 and p6, bind synergistically to DNA, modifying the topology of the sequences encompassing early promoters A2c and A2b and late promoter A3 in a hairpin that allows the switch from early to late transcription. Protein p6 is a nucleoid-like protein that binds DNA in a non-sequence specific manner. Protein p4 is a sequence-specific DNA binding protein with multifaceted sequence-readout properties. The protein recognizes the chemical signature of only one DNA base on the inverted repeat of its target sequence through a direct-readout mechanism. In addition, p4 specific binding depends on the recognition of three A-tracts by indirect-readout mechanisms. The biological importance of those three A-tracts resides in their individual properties rather than in the global curvature that they may induce.
Keywords: transcription regulator; nucleoprotein complex; protein-DNA recognition; direct-indirect-readout; protein-induced DNA hairpin; A-track; minor groove malleability; arginine in minor groove transcription regulator; nucleoprotein complex; protein-DNA recognition; direct-indirect-readout; protein-induced DNA hairpin; A-track; minor groove malleability; arginine in minor groove
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Camacho, A.; Salas, M. Molecular Interactions and Protein-Induced DNA Hairpin in the Transcriptional Control of Bacteriophage Ø29 DNA. Int. J. Mol. Sci. 2010, 11, 5129-5142.

AMA Style

Camacho A, Salas M. Molecular Interactions and Protein-Induced DNA Hairpin in the Transcriptional Control of Bacteriophage Ø29 DNA. International Journal of Molecular Sciences. 2010; 11(12):5129-5142.

Chicago/Turabian Style

Camacho, Ana; Salas, Margarita. 2010. "Molecular Interactions and Protein-Induced DNA Hairpin in the Transcriptional Control of Bacteriophage Ø29 DNA." Int. J. Mol. Sci. 11, no. 12: 5129-5142.


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