Int. J. Mol. Sci. 2010, 11(11), 4452-4464; doi:10.3390/ijms11114452
Article

Comparison of the Phenolic Content and Antioxidant Activities of Apocynum venetum L. (Luo-Bu-Ma) and Two of Its Alternative Species

School of Pharmaceutical Science, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China
* Author to whom correspondence should be addressed.
Received: 30 September 2010; in revised form: 17 October 2010 / Accepted: 1 November 2010 / Published: 9 November 2010
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: The leaves of Apocynum venetum L. (AV), a native Chinese plant, have been used as folk medicine in China and Japan. This study evaluated the content of the active antioxidant component and antioxidant activities of AV, and its two alternative species, Poacynum pictum (Schrenk) Baill. (PP) and Poacynum hendersonii (Hook.f.) Woodson (PH). The total phenolic and total flavonoid contents were determined. In addition, the quantitative analysis of two major flavonoid compounds (hyperoside and isoquercitrin) was carried out by HPLC. The antioxidant activities were investigated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity method, the reducing power test and the chelating ability of ferrous ions. The highest total phenolic and flavonoid contents were observed in the AV methanolic extract, followed by the PP and PH methanolic extracts. HPLC analysis indicated that isoquercitrin was one of the major components in all three species, however, hyperoside was only detected in AV at high levels. All the antioxidant assays we performed demonstrated that the AV extract was markedly superior to those of the other two species.
Keywords: Apocynum venetum L.; Poacynum pictum (Schrenk) Baill.; Poacynum hendersonii (Hook.f.) Woodson; antioxidant activity; phenolic; flavonoid; HPLC

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MDPI and ACS Style

Liang, T.; Yue, W.; Li, Q. Comparison of the Phenolic Content and Antioxidant Activities of Apocynum venetum L. (Luo-Bu-Ma) and Two of Its Alternative Species. Int. J. Mol. Sci. 2010, 11, 4452-4464.

AMA Style

Liang T, Yue W, Li Q. Comparison of the Phenolic Content and Antioxidant Activities of Apocynum venetum L. (Luo-Bu-Ma) and Two of Its Alternative Species. International Journal of Molecular Sciences. 2010; 11(11):4452-4464.

Chicago/Turabian Style

Liang, Taigang; Yue, Wenyan; Li, Qingshan. 2010. "Comparison of the Phenolic Content and Antioxidant Activities of Apocynum venetum L. (Luo-Bu-Ma) and Two of Its Alternative Species." Int. J. Mol. Sci. 11, no. 11: 4452-4464.

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