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Lewis Y Promotes Growth and Adhesion of Ovarian Carcinoma-Derived RMG-I Cells by Upregulating Growth Factors
Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, 36 Sanhao Street, Heping, Shenyang, 110004, China
Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan, China
Department of Obstetrics and Gynecology, Maternity and Children Healthcare Center of Shenyang, Shenyang, China
* Author to whom correspondence should be addressed.
Received: 9 August 2010; in revised form: 13 September 2010 / Accepted: 16 September 2010 / Published: 29 September 2010
Abstract: Lewis y (LeY) antigen is a difucosylated oligosaccharide carried by glycoconjugates on the cell surface. Overexpression of LeY is frequently observed in epithelial-derived cancers and has been correlated to the pathological staging and prognosis. However, the effects of LeY on ovarian cancer are not yet clear. Previously, we transfected the ovarian cancer cell line RMG-I with the α1,2-fucosyltransferase gene to obtain stable transfectants, RMG-I-H, that highly express LeY. In the present study, we examined the proliferation, tumorigenesis, adhesion and invasion of the cell lines with treatment of LeY monoclonal antibody (mAb). Additionally, we examined the expression of TGF-β1, VEGF and b-FGF in xenograft tumors. The results showed that the proliferation and adhesion in vitro were significantly inhibited by treatment of RMG-I-H cells with LeY mAb. When subcutaneously inoculated in nude mice, RMG-I-H cells produced large tumors, while mock-transfected cells RMG-I-C and the parental cells RMG-I produced small tumors. Moreover, the tumor formation by RMG-I-H cells was inhibited by preincubating the cells with LeY mAb. Notably, the expression of TGF-β1, VEGF and b-FGF all increased in RMG-I-H cells. In conclusion, LeY plays an important role in promoting cell proliferation, tumorigenecity and adhesion, and these effects may be related to increased levels of growth factors. The LeY antibody shows potential application in the treatment of LeY-positive tumors.
Keywords: Lewis y; ovarian cancer; proliferation; tumorigenecity; adhesion; inhibition
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Li, F.; Lin, B.; Hao, Y.; Li, Y.; Liu, J.; Cong, J.; Zhu, L.; Liu, Q.; Zhang, S. Lewis Y Promotes Growth and Adhesion of Ovarian Carcinoma-Derived RMG-I Cells by Upregulating Growth Factors. Int. J. Mol. Sci. 2010, 11, 3748-3759.
Li F, Lin B, Hao Y, Li Y, Liu J, Cong J, Zhu L, Liu Q, Zhang S. Lewis Y Promotes Growth and Adhesion of Ovarian Carcinoma-Derived RMG-I Cells by Upregulating Growth Factors. International Journal of Molecular Sciences. 2010; 11(10):3748-3759.
Li, Feifei; Lin, Bei; Hao, Yingying; Li, Yan; Liu, Juanjuan; Cong, Jianping; Zhu, Liancheng; Liu, Qing; Zhang, Shulan. 2010. "Lewis Y Promotes Growth and Adhesion of Ovarian Carcinoma-Derived RMG-I Cells by Upregulating Growth Factors." Int. J. Mol. Sci. 11, no. 10: 3748-3759.