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Int. J. Mol. Sci. 2010, 11(1), 173-188; doi:10.3390/ijms11010173
Article

Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion

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Received: 1 December 2009; in revised form: 31 December 2009 / Accepted: 4 January 2010 / Published: 12 January 2010
(This article belongs to the Special Issue Single Molecules)
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Abstract: The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 104 M-1 are obtained in aqueous solution at 25 °C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.
Keywords: fluorescence correlation spectroscopy; host-guest chemistry; adamantane; cyclodextrin fluorescence correlation spectroscopy; host-guest chemistry; adamantane; cyclodextrin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Granadero, D.; Bordello, J.; Pérez-Alvite, M.J.; Novo, M.; Al-Soufi, W. Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion. Int. J. Mol. Sci. 2010, 11, 173-188.

AMA Style

Granadero D, Bordello J, Pérez-Alvite MJ, Novo M, Al-Soufi W. Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion. International Journal of Molecular Sciences. 2010; 11(1):173-188.

Chicago/Turabian Style

Granadero, Daniel; Bordello, Jorge; Pérez-Alvite, Maria Jesus; Novo, Mercedes; Al-Soufi, Wajih. 2010. "Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion." Int. J. Mol. Sci. 11, no. 1: 173-188.



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