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Int. J. Mol. Sci. 2010, 11(1), 173-188; doi:10.3390/ijms11010173

Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion

1
Departamento de Química Física, Facultade de Ciencias, Universidade de Santiago de Compostela, E 27002 Lugo, Spain
2
Departamento de Química Orgánica, Facultade de Química, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain
*
Author to whom correspondence should be addressed.
Received: 1 December 2009 / Revised: 31 December 2009 / Accepted: 4 January 2010 / Published: 12 January 2010
(This article belongs to the Special Issue Single Molecules)
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Abstract

The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 104 M-1 are obtained in aqueous solution at 25 °C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.
Keywords: fluorescence correlation spectroscopy; host-guest chemistry; adamantane; cyclodextrin fluorescence correlation spectroscopy; host-guest chemistry; adamantane; cyclodextrin
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MDPI and ACS Style

Granadero, D.; Bordello, J.; Pérez-Alvite, M.J.; Novo, M.; Al-Soufi, W. Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion. Int. J. Mol. Sci. 2010, 11, 173-188.

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