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Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.
Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brazil
Laboratório de Substâncias Antitumorais, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil
* Author to whom correspondence should be addressed.
Received: 26 July 2009; Accepted: 26 August 2009 / Published: 26 August 2009
Abstract: In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC50 value of 31.25 μg/mL. The AE had a promising activity against mouse melanoma (B16F1), human breast cancer (MDA MB-231) and rat glioma (C6) cell lines, with IC50 values of 698.0, 648.0 and 1000.0 μg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.
Keywords: Centella asiatica; Umbelliferae; phenolic constituents; DPPH; cytotoxic
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Pittella, F.; Dutra, R.C.; Junior, D.D.; Lopes, M.T.P.; Barbosa, N.R. Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.. Int. J. Mol. Sci. 2009, 10, 3713-3721.
Pittella F, Dutra RC, Junior DD, Lopes MTP, Barbosa NR. Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.. International Journal of Molecular Sciences. 2009; 10(9):3713-3721.
Pittella, Frederico; Dutra, Rafael C.; Junior, Dalton D.; Lopes, Miriam T. P.; Barbosa, Nádia R. 2009. "Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.." Int. J. Mol. Sci. 10, no. 9: 3713-3721.