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Int. J. Mol. Sci. 2009, 10(9), 3713-3721; doi:10.3390/ijms10093713

Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.

1, 2, 2
1 Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brazil 2 Laboratório de Substâncias Antitumorais, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil
* Author to whom correspondence should be addressed.
Received: 26 July 2009 / Accepted: 26 August 2009 / Published: 26 August 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC50 value of 31.25 μg/mL. The AE had a promising activity against mouse melanoma (B16F1), human breast cancer (MDA MB-231) and rat glioma (C6) cell lines, with IC50 values of 698.0, 648.0 and 1000.0 μg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.
Keywords: Centella asiatica; Umbelliferae; phenolic constituents; DPPH; cytotoxic Centella asiatica; Umbelliferae; phenolic constituents; DPPH; cytotoxic
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pittella, F.; Dutra, R.C.; Junior, D.D.; Lopes, M.T.P.; Barbosa, N.R. Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.. Int. J. Mol. Sci. 2009, 10, 3713-3721.

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