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Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China
Department of Theoretic Veterinary Medicine, Colledge of Animal Science and Veterinary, Jilin University, Changchun 130062, China
* Author to whom correspondence should be addressed.
Received: 27 April 2009; in revised form: 14 May 2009 / Accepted: 15 May 2009 / Published: 22 May 2009
Abstract: The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.
Keywords: ricin; BBM; SPR; HPCE-ESI-MS
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Liu, L.; Gao, H.; Li, J.; Dong, Y.; Liu, N.; Wan, J.; Liu, W.; Sun, Y.; Xu, M. Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification. Int. J. Mol. Sci. 2009, 10, 2431-2439.
Liu L, Gao H, Li J, Dong Y, Liu N, Wan J, Liu W, Sun Y, Xu M. Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification. International Journal of Molecular Sciences. 2009; 10(5):2431-2439.
Liu, Linna; Gao, Hongwei; Li, Jiping; Dong, Ying; Liu, Ning; Wan, Jiayu; Liu, Wensen; Sun, Yucheng; Xu, Ming. 2009. "Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification." Int. J. Mol. Sci. 10, no. 5: 2431-2439.