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Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 26 March 2009; in revised form: 15 April 2009 / Accepted: 17 April 2009 / Published: 6 May 2009
Abstract: The objective of this study was to elucidate the effects of hydroxylpropyl-β-cyclodextrin (HP-β-CD) on the in vitro stability of insulin. It was found that HP-β-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-β-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through 1H-NMR experiments it was found that the protective effect of HP-β-CD was due to complexation with insulin. The results suggest that the presence of HP-β-CD could improve the stability of insulin in different environments.
Keywords: Protection; insulin; stability; cyclodextrin
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Zhang, L.; Zhu, W.; Song, L.; Wang, Y.; Jiang, H.; Xian, S.; Ren, Y. Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability. Int. J. Mol. Sci. 2009, 10, 2031-2040.
Zhang L, Zhu W, Song L, Wang Y, Jiang H, Xian S, Ren Y. Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability. International Journal of Molecular Sciences. 2009; 10(5):2031-2040.
Zhang, Liefeng; Zhu, Wenjie; Song, Lingling; Wang, Yifan; Jiang, Hui; Xian, Suyun; Ren, Yong. 2009. "Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability." Int. J. Mol. Sci. 10, no. 5: 2031-2040.