- freely available
Int. J. Mol. Sci. 2009, 10(3), 1369-1385; doi:10.3390/ijms10031369
Published: 24 March 2009
Abstract: Based on the differences in their optimal growth temperatures microorganisms can be classified into psychrophiles, mesophiles, thermophiles, and hyperthermophiles. Proteins from hyperthermophiles generally exhibit greater stability than those from other organisms. In this review, we collect data about the stability and folding of monomeric proteins from hyperthermophilies with reversible unfolding, from the equilibrium and kinetic aspects. The results indicate that slow unfolding is a general strategy by which proteins from hyperthermophiles adapt to higher temperatures. Hydrophobic interaction is one of the factors in the molecular mechanism of the slow unfolding of proteins from hyperthermophiles.
Many organisms on Earth grow in extreme environments. Microorganisms whose optimal growth temperature is above 80 °C are called hyperthermophiles. Hyperthermophiles are found in hot environments such as deep-sea vents, submarine hydrothermal areas, and continental solfataras . Most hyperthermophiles belong to archaea (e.g. Thermococcus, Pyococcus, and Sulfolobus), but some hyperthermophiles from bacteria (Thermotoga and Aquifex) have also been discovered.
Proteins from hyperthermophiles usually exhibit higher stability than those from organisms that grow at lower temperatures, so it is expected that studies of proteins from hyperthermophiles will provide general or additional insights into the forces stabilizing the native conformation of proteins . In recent years, many genomes from hyperthermophiles have been sequenced, and a number of the crystal structures of proteins from hyperthermophiles have been determined. This enables us to compare the sequences and the crystal structures of homologous proteins between hyperthermophiles and other organisms growing in moderate temperatures. These comparative studies indicate that the higher stability of proteins from hyperthermophiles is associated with several factors such as increased salt bridges, improved hydrogen bonding, favorable packing interactions, fewer cavities, and improved hydrophobic interactions [3–7]. It has been suggested that proteins from hyperthermophiles use various combinations of these stabilizing factors.
To elucidate the stabilization mechanism of proteins from hyperthermophiles, a thermodynamic analysis is also useful [8–11]. Many studies concerning the thermodynamic stability of proteins from hyperthermophiles focus primarily on the equilibrium aspects, which reveal that extremely high stability can be achieved in these proteins by increasing the number of ionic interactions and the extent of hydrophobic surface burial [12–14]. Furthermore, it has recently been reported that the stability of some proteins is under kinetic control in vivo and in vitro [15–22]. Luke et al.  summarized the data on thermodynamic and kinetic folding behavior of proteins from hyperthermophiles and found that the unfolding is slower for proteins from hyperthermophiles than for the mesostable ones. The data, however, includes data from oligomeric or monomeric proteins with irreversible or multi-state folding.
This review focuses on studies of monomeric proteins from hyperthermophiles with reversible unfolding, examined from both equilibrium and kinetic aspects. This will provide an essential understanding of stabilization linked to the slow unfolding of proteins from hyperthermophiles. In the following section, we describe how the stability and folding of proteins from hyperthermophiles are experimentally characterized by thermodynamic and kinetic analyses. Next, we report on our studies of the stability and folding of ribonuclease HII from a hyperthermophilic archaeon, Thermococcus kodakaraensis (Tk-RNase HII) and introduce other studies of proteins from hyperthermophiles. We conclude that slower unfolding is a fundamental characteristic of the architectural principles of proteins from hyperthermophiles. Moreover, we discuss the molecular mechanism of the slow unfolding of proteins from hyperthermophiles.
2. Characterizing the Stability and Folding of Hyperthermophilic Proteins
The stability of proteins in solution is evaluated quantitatively by Gibbs energy changes (ΔG) observed upon unfolding, when the reaction is reversible under experimental conditions. ΔG is obtained experimentally by perturbing the native state using temperature and a denaturant (e.g., urea or guanidine hydrochloride (GdnHCl)) and monitoring the shift in the equilibrium by various biophysical techniques such as differential scanning calorimetry (DSC), fluorescence, and circular dichroism (CD) . Assuming a simple two-state process (Native, N ⇌ Unfolded, U), ΔG is given by:where R, T, and K represent the gas constant, temperature, and equilibrium constant. The temperature dependence of ΔG (stability profile) provides more valuable information about the thermodynamic stability of proteins. The temperature dependence of ΔG is expressed as: where ΔHm is the enthalpy of unfolding at the transition midpoint temperature (denaturation temperature: Tm) and ΔCp is the difference in heat capacity between the native and unfolded states.
Three models are proposed for proteins from hyperthermophiles to adapt to higher temperatures compared with those from organisms growing in moderate temperatures: Proteins from hyperthermophiles (i) raises the entire stability curve to a higher ΔG, (ii) shifts the stability curve towards higher temperatures, and (iii) reduces ΔCp, resulting in a flatter stability curve (Figure 1).
All three models are observed in nature and, in many cases, proteins from hyperthermophiles do not utilize a single mechanism but rather various combinations of (i) to (iii) [25–31]. It has been experimentally shown that the core hydrophobicity and electrostatic interaction at the surface in proteins from hyperthermophiles are involved in the temperature dependence of ΔG [13,30]. For example, ribosomal protein L30e from a hyperthermophile, Thermococcus celer, adapts to higher temperatures by utilizing models (i) and (iii) compared with its mesophilic homologue from yeast and the mutational study reveals that electrostatic interactions contribute to the greater stability and reduced ΔCp of L30e from Thermococcus celer .
ΔG is also determined by kinetic unfolding/folding experiments. In kinetic experiments, a perturbation is imposed by changing the denaturant concentration, pH, or temperature. The time course of the reaction is then monitored by the various biophysical techniques described above . Assuming a protein that displays a simple two-state transition, the reactions of unfolding and refolding curves are fit to a single exponential, yielding a single apparent rate constant kapp where kapp = kunf + kref, and kunf and kref represent the unfolding and refolding rate constants. For the unfolding and refolding kinetics of a denaturation concentration jump, the logarithm of the apparent rate constant (kapp) of the unfolding and refolding linearly depends on the final denaturant concentration. The dependence of ln kapp on the denaturant concentration is generally analyzed using the following equation:
Here, kunf(H2O) and kref(H2O) represent the unfolding and refolding rate constants in the absence of denaturant and munf and mref are their dependencies on the denaturant concentration. [D] is the final denaturant concentration. Importantly, ΔG is also obtained using the rate constants of unfolding and refolding reactions. For a simple two-state transition, the equilibrium constant (K) is expressed as the ratio of the refolding rate constant to the unfolding one (K = kf/ku).
Figure 2 displays a Gibbs energy diagram of the folding pathway for protein, assuming a two-state model. Hence, the increased stability of proteins from hyperthermophiles is caused by the slower unfolding rate (the larger ΔG#unfolding value), the faster refolding rate (the smaller ΔG#folding value), or both.
3. Ribonuclease HII from Thermococcus kodakaraensis
Tk-RNase HII is a monomeric protein consisting of 228 amino acid residues whose stability and folding are well characterized by our research [20,33–36]. RNase H hydrolyzes only the RNA strand of an RNA/DNA hybrid . The enzyme is ubiquitously present in various organisms and is involved in DNA replication and repair . The crystal structures of Tk-RNase HII and its several variants have been determined [39–41]. The protein contains an α/β fold, known as the RNase H-fold due to its characterization in Escherichia coli RNase HI. Tk-RNase HII exhibits high reversibility against both heat- and GdnHCl-induced unfolding. Equilibrium unfolding by GdnHCl is a two-state process, with a reaction at 50 °C attaining equilibrium in two weeks (Figure 3a). ΔG in water (ΔG(H2O)) at 50 °C, obtained from the equilibrium unfolding curve, is 43.6kJ mol−1, indicating that this protein is very stable at 50 °C compared to mesophilic proteins.
The dependence of ΔG on the GdnHCl concentration (m value) is 23.6kJ mol−1 M−1. However, the unfolding curve is inconsistent with the refolding curve at 20 °C even after 30 days (Figure 3b). The inconsistency between the refolding and unfolding curves is due to the remarkably slow unfolding of Tk-RNase HII. It takes about two months to reach equilibrium at 20 °C.
The dependence of ΔG(H2O) on the temperature (stability profile) for Tk-RNase HII was investigated and compared with that for the mesophilic bacterium Escherichia coli (Ec-RNase HI) and the thermophilic bacterium Thermus thermophilus (Tt-RNase HI) . Figure 4 plots the stability profiles for the three proteins. Tt-RNase HI increases the stability by shifting the stability curve up and flattening it compared with Ec-RNase HI. The stability profile for Tk-RNase HII displays a maximum around 40 °C, which is higher than that of Ec-RNase HI and Tt-RNase HI. The ΔCp of Tk-RNase HII, 14.5kJ mol−1 K−1, exceeds that of Tt-RNase HI. Therefore, Tk-RNase HII adapts to higher temperature by shifting the stability curve up and to the right. The results suggest that the stabilization mechanisms of Tk-RNase HII and Tt-RNase HI are different.
Heat-induced unfolding experiments with Tk-RNase HII were carried out by DSC. The results demonstrate the high stability and slow unfolding of Tk-RNase HII. Figure 5(a) presents DSC curves for Tk-RNase HII at scan rates of 30 and 60 °C hour−1. The denaturation temperature in the DSC curve for Tk-RNase HII shifts as a function of the scan rate. The denaturation temperature at a scan rate of 90 °C hour−1 is 89.2 °C, whereas the denaturation temperature at 5 °C hour−1 is 87.2 °C. This indicates that the heat-induced unfolding of Tk-RNase HII does not attain equilibrium at these scan rates because of the remarkably slow unfolding. Heat-induced unfolding of most mesophilic proteins can attain equilibrium at 60 °C hour−1. Figure 5(b) plots the dependence of the denaturation temperature as a function of the scan rate. The extrapolated value is 87.1 °C. Although the linear fit is not theoretical, this value indicates that Tk-RNase HII is very stable against heat-induced denaturation.
The unfolding and refolding kinetics by GdnHCl concentration jump was examined at 50 °C. Figure 6(a) shows the typical unfolding curves of Tk-RNase HII. The observed kinetics of unfolding and refolding reaction is fit to a single exponential. Figure 6(b) shows the dependence of the logarithm of the apparent rate constant of the unfolding and refolding on the final GdnHCl concentration. The kunf(H2O) and kref(H2O) at 50 °C are 5.0 × 10−8 s−1 and 7.8 × 10−1 s−1, and munf and mref at 50 °C are 2.8 M−1 s−1 (7.5 kJ mol−1 M−1) and 5.5 M−1 s−1 (14.8 kJ mol−1 M−1), respectively. The ΔG(H2O) value obtained from those rate constants using a two-state model at 50 °C, 44.5 kJ mol−1, is coincident with that from the equilibrium study, 43.6 kJ mol−1. This suggests two-state folding/unfolding of Tk-RNase HII. The fractional change of the m value during refolding α, where β = mref / (mref - munf), yields 0.66, indicating that the transition state of folding/unfolding is similar with the native state rather than the unfolded state in its solvent accessible area .
The kinetics of the folding and refolding reactions of Ec-RNase HII and Tt-RNase HI have been studied in detail at 25 °C [44,45]. To compare the unfolding and refolding rate constants for Tk-RNase HII with those for Ec-RNase HII and Tt-RNase HI, the kinetic experiments were also carried out at 25 °C. The unfolding rate constant for Tk-RNase HII at 25 °C (6.0 × 10−10s−1) is much smaller than those for Ec-RNase HI (1.1 × 10−5s−1) and Tt-RNase HI (4.0 × 10−6s−1). In contrast, little difference is observed among these proteins in the refolding rate constant for the formation of the native state. These results indicate that the stabilization of Tk-RNase HII originates from the remarkably slow unfolding rate.
4. Other Proteins
4.1. Cold shock protein (Csp)
The stability and folding of cold shock proteins from Thermotoga maritima (hyperthermophilic bacterium, Tm-Csp), Bacillus caldolyticus (thermophilic bacterium, Bc-Csp), and Bacillus subtilis (mesophilic bacterium, Bs-Csp) have been investigated in detail . These proteins are small (66 to 68 amino acid residues) and adopt all-0 sheet folds in their native structures. GdnHCl-induced equilibrium unfolding is a two-state process, and the kinetic intermediates are not accumulated during the unfolding and refolding reactions (20 °C). Tm-Csp exhibits higher stability (26.2kJ mol−1) than Bc-Csp (20.1kJ mol−1) and Bs-Csp (11.3kJ mol−1). Coulombic interactions at the surface unique to Tm-Csp are involved in an increase in the stability . The fractional change of the m value during folding/unfolding (α value) was 0.86 for Tm-Csp and 0.93 for Bc-Csp, indicating that the transition states of refolding of all cold shock proteins are almost native-like in their interactions with the solvent. The unfolding rate constant of Tm-Csp is one order of magnitude smaller than that of Bc-Csp and two orders of magnitude smaller than that of Bs-Csp, while the refolding rate constants are similar for the three proteins. An increase in the equilibrium stability of Tm-Csp is brought about by the slower unfolding. The slower unfolding for Tm-Csp relative to Bs-Csp is observed over a wide temperature range. The difference is found to be due to a difference in the activation entropy of unfolding .
4.2. Ribosomal protein S16
The folding thermodynamics and kinetics of ribosomal protein S16 from the hyperthermophilic bacterium Aquifex aerolicus (S16thermo) have been investigated and compared with those of ribosomal protein S16 from the mesophilic bacterium Chlamydia pneumoniae (S16meso) . S16thermo consists of 112 amino acid residues, and its crystal structure has been determined. Thermal unfolding experiments demonstrate that the Tm values for S16thermo and S16meso are 111 °C and 59 ° C. Equilibrium unfolding by urea of S16thermo and S16meso is a two-state process. The stability profile of S16thermo is shifted up and flattened, resulting in a reduced ACp, and shifted toward higher temperatures than that of S16meso. The unfolded state of S16thermo is more compact relative to S16meso, suggesting that residual structures in the unfolded state of S16thermo are involved in the increased stability. The unfolding rate constant for S16meso is two orders of magnitude smaller than that of S16meso, indicating that the difference in unfolding rate accounts for the difference in stability between the two proteins. For refolding, the rate constants for the formation of the native state are similar for both proteins.
5. Molecular Mechanisms of the Slow Unfolding of Hyperthermophilic Proteins
From the data presented above, slower unfolding seems to be a common characteristic of proteins from hyperthermophiles. However, the molecular basis for the slow unfolding of proteins from hyperthermophiles remains elusive. Here, we describe our recent studies in which the contributions of various stabilization factors to the slower unfolding of Tk-RNase HII are investigated. Moreover, we propose a novel viewpoint for future work concerning the evolutionary background of the slow unfolding of proteins from hyperthermophiles.
5.1. Hydrophobic interactions
The hydrophobic effect is one of the important stabilizing forces of folded proteins [50–54]. Systematic mutational analysis reveals that buried hydrophobic residues contribute to the extremely slow unfolding of Tk-RNase HII . Mutant proteins in which a larger hydrophobic side chain is replaced by a smaller one (Lue/Ile to Ala) are destabilized by 8.9 to 22.0 kJ mol−1 at 50 °C. The unfolding rate constants of these mutant proteins are one to three orders of magnitude greater than that of the wild-type protein (Figure 7). These mutant proteins refold more slowly, but the difference is less than one order of magnitude. The mutation sites are completely buried, with their locations distributed widely throughout the molecule (Figure 8). These results indicate that buried hydrophobic residues strongly contribute to the kinetic robustness of Tk-RNase HII. This is the first report that presents a practical cause for the slow unfolding of proteins from hyperthermophiles. In contrast, it has been reported from simulation studies that protein-water interactions at protein surface rather than internal packing are important for the stabilization of proteins from hyperthermophiles [56,57]. Today, it remains unclear whether protein-water interactions at the surface contribute to the slow unfolding of proteins from hyperthermophiles, and it is an interesting subject which should be solved.
5.2. The Proline Effect
Proline residues decrease the conformational entropy of the denatured state and consequently lead to protein stabilization . In particular, it has been reported that proline residues introduced at N-terminal α-helices increase the thermostability of proteins . From sequence comparisons among homologous proteins with different thermostability, proline residues are more ubiquitous at α-helix N-terminals in more stable proteins . Hence, the effects of proline residues at the N-terminal α-helices on the stability and slow unfolding of Tk-RNase HII have been tested by mutational analysis . The results indicate that Tk-RNase HII is stabilized by proline residues at the N-terminal α-helices. In contrast, the unfolding rate for the mutant proteins changes less than one order of magnitude compared with that of the wild-type protein, indicating that proline residues contribute little to the slow unfolding of Tk-RNase HII.
5.3. The osmolyte effect
The effects of osmolyte on the stability and folding of Tk-RNase HII have been investigated . Osmolytes are small molecules that protect plants, animals and microorganisms, including hyperthermophiles, from harsh environmental stress [60,61]. The presence of the osmolyte trimethyl-amine-N-oxide (TMAO) in solution increases the stability of Tk-RNase HII. Kinetic experiments indicate that the ku(H2O) value in the presence of 0.5M TMAO is about one-seventh of that in the absence of TMAO, and that the kr(H2O) value in the presence of 0.5M TMAO is about seven times higher than that in the absence of TMAO, indicating that TMAO affects the unfolding and refolding kinetics of Tk-RNase HII to a similar extent. Since similar effects of osmolytes on unfolding and refolding rates have been observed in several proteins from humans and mesophiles [62,63], a decrease in unfolding rate by osmolytes is not intrinsic for proteins from hyperthermophiles.
5.4. Evolutionary background of the slow unfolding of hyperthermophilic proteins
The degree of the decrease in unfolding rate of proteins from hyperthermophiles relative to their mesophilic counterparts varies, depending on the protein. For example, the unfolding rate constant for Tk-RNase HII is much less (by five orders of magnitude) than that for its mesophilic counterpart. By contrast, the unfolding rate constant for Tm-Csp and S16thermo is slightly smaller (by two orders of magnitude) than that of its mesophilic homolog. The origin of such a difference remains obscure at this point. Here, we propose one possibility for this discrimination.
Thermococcus kodakaraensis belongs to archaea , but Thermotoga maritima and Aquifex aeolicus are bacteria [65,66]. Archaea and bacteria divided at an early stage of the evolutionary tree [67,68]. It has been proposed that their proteins have different stabilization mechanisms . Proteins from archaea that originated in a hot environment are more compact and hydrophobic than their mesophilic homologs. In contrast, proteins from some bacteria that later recolonized under extreme conditions are stabilized by specific interactions such as salt bridges. The difference in the unfolding characteristics of proteins from hyperthermophiles may be ascribed to this difference in stabilization mechanism between archaea and bacteria. The strong effect of hydrophobic interaction on the slow unfolding of Tk-RNase HII supports this hypothesis. We will confirm this in the future.
Previous studies showed that the unfolding is slower for proteins from hyperthermophiles than for the mesostable ones, as summarized in the review by Luke et al. . The results, however, includes data from oligomer or monomer proteins with irreversible or multi-state folding. In this review, we focus on monomeric proteins from hyperthermophiles with reversible unfolding examined in terms of the equilibrium and kinetic aspects. Unfortunately, the number of studies is quite low. Table 1 provides thermodynamic and kinetic data for proteins from hyperthermophiles described in this review. More studies are required to clarify the universal mechanism of the stabilization of proteins from hyperthermophiles. However, we found a clear trend whereby the stabilization mechanism of proteins from hyperthermophiles originates from slow unfolding, based on data from monomeric proteins with reversible unfolding. This means that the slow unfolding is a basic characteristic of the architectural principles of proteins from hyperthermophiles, because other factors such as oligomerization and uncertainties due to irreversible reactions can be eliminated.
- Charlier, D; Droogmans, L. Microbial life at high temperature, the challenge, the strategies. Cell. Mol. Life Sci 2005, 62, 2974–2984. [Google Scholar]
- Vieille, C; Zeikus, GJ. Hyperthermophilic enzymes: sources, uses and molecular mechanisms for thermostability. Microbiol. Mol. Biol. Rev 2001, 65, 1–43. [Google Scholar]
- Elcock, AH. The stability of salt bridges at high temperatures: Implications for hyper-thermophilic proteins. J. Mol. Biol 1998, 284, 489–502. [Google Scholar]
- Szilagyi, A; Zavodszky, P. Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: results of a comprehensive survey. Structure 2000, 8, 493–504. [Google Scholar]
- Cambillau, C; Claverie, JM. Structural and genomic correlates of hyperthermostability. J. Biol. Chem 2000, 275, 32383–32386. [Google Scholar]
- Ree, DC. Crystallographic analyses of hyperthermophilic proteins. Methods Enzymol 2001, 334, 423–437. [Google Scholar]
- Matsui, I; Harata, K. Implication forburied polar contacts and ion pairs in hyperthermostable enzymes. FEBS J 2007, 274, 4012–4022. [Google Scholar]
- Jaenicke, R; Böhm, G. The stability of proteins in extreme environments. Curr. Opin. Struct. Biol 1998, 8, 738–748. [Google Scholar]
- Kumar, S; Tsai, CJ; Nussinov, R. Factors enhancing protein thermostability. Protein Eng 2000, 13, 179–191. [Google Scholar]
- Kumar, S; Nussinov, R. How do thermophilic proteins deal with heat? Cell. Mol. Life Sci 2001, 58, 1216–1233. [Google Scholar]
- Razvi, A; Scholtz, JM. Lessons in stability from thermophilic proteins. Protein Sci 2006, 15, 1569–1578. [Google Scholar]
- Ruiz-Sanz, J; Filimonov, VV; Christodoulou, E; Vorgias, CE; Mateo, PL. Thermodynamic analysis of the unfolding and stability of the dimericDNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant. Eur. J. Biochem 2004, 271, 1497–1507. [Google Scholar]
- Clark, AT; McCrary, BS; Edmondson, SP; Shriver, JW. Thermodynamics of core hydrophobicity and packing in the hyperthermophile proteins Sac7d and Sso7d. Biochemisty 2004, 43, 2840–2853. [Google Scholar]
- Ge, M; Xia, XY; Pan, XM. Salt bridges in the hyperthermophilic protein Ssh10b are resilient to temperature incrases. J. Bio. Chem 2008, 283, 31690–31696. [Google Scholar]
- Dams, T; Jaenicke, R. Stability and folding of dihydrofolate reductase from the hyperthermophilic bacterium Thermotoga maritima. Biochemistry 1999, 38, 9169–9178. [Google Scholar]
- Ogasahara, K; Nakamura, M; Nakura, S; Tsunasawa, S; Kato, I; Yoshimoto, T; Yutani, K. The unusually slow unfolding rate causes the high stability of pyroolidone carboxyl peptidase from a hyperthermophilile Pyrococcus furiosus: equilibrium and kinetic studies of guanidine hydrochloride-induced unfolding and refolding. Biochemistry 1998, 37, 17537–17544. [Google Scholar]
- Jaswal, SS; Sohl, JL; Dans, JH; Agard, DA. Energetic landscape of alpha-lytic protease optimizes longevity through kinetic stability. Nature 2002, 415, 343–346. [Google Scholar]
- Kaushik, JK; Ogasahara, K; Yutani, K. The unusually slow relaxation kinetics of the folding-unfolding of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus. J. Mol. Biol 2002, 316, 991–1003. [Google Scholar]
- Iimura, S; Yagi, H; Ogasahara, K; Akutsu, H; Noda, Y; Segawa, S; Yutani, K. Unusually slow denaturation and refolding process of pyrrolidone carcoxyl peptidase from a hyper-thermophile are highly cooperative: Real-time NMR studies. Biochemistry 2004, 43, 11906–11915. [Google Scholar]
- Mukaiyama, A; Takano, K; Haruki, M; Morikawa, M; Kanaya, S. Kinetically robust monomeric protein from a hyperthermophile. Biochemistry 2004, 43, 13859–13866. [Google Scholar]
- Forrer, P; Chang, C; Ott, D; Wlodawer, A; Plückthun, A. Kinetic stability and crystal structure of the viral capside protein SHP. J. Mol. Biol 2004, 344, 179–193. [Google Scholar]
- Kaushik, JK; Iimura, S; Ogasahara, K; Yamagata, Y; Segawa, S; Yutani, K. Completely buried, non-ion-paired glutamic acid contributes favorably to the conformational stability of pyrrolidone carboxyl peptidases from hyperthermophiles. Biochemistry 2006, 45, 7100–7112. [Google Scholar]
- Luke, KA; Higgins, CL; Wittung-Stafshede, P. Thermodynamic stability and folding of proteins from hyperthermophilic organisms. FEBS J 2007, 274, 4023–4033. [Google Scholar]
- Pace, CN. Measuring and increasing protein stability. Trends Biotechnol 1990, 8, 93–98. [Google Scholar]
- McCrary, BS; Edmondson, SP; Shriver, JW. Hyperthermophile protein folding thermodynamics: Differential scanning calorimetry and chemical denaturation of Sac7d. J. Mol. Biol 1996, 264, 784–805. [Google Scholar]
- Li, WT; Grayling, RA; Sandman, K; Edmondson, S; Shriver, JW; Reeve, JN. Thermodynamic stability of archaeal histones. Biochemistry 1998, 37, 10563–10572. [Google Scholar]
- Grättinger, M; Dankesreiter, A; Schurig, H; Jaenicke, R. TRecombinant phosphoglycerate kinase from the hyperthermophilic bacterium Thermotoga maritima: Catalytic, spectral and thermodynamic properties. J. Mol. Biol 1998, 280, 525–533. [Google Scholar]
- Shiraki, K; Nishikori, S; Fujiwara, S; Hashimoto, H; Kai, Y; Takagi, M; Imanaka, T. Comparative analyses of the conformational stability of a hyperthermophilic protein and its mesophilic counterpart. Eur. J. Biochem 2001, 268, 4144–4150. [Google Scholar]
- Deutschman, WA; Dahlquist, FW. Thermodynamic basis for the increased thermostability of CheY from the hyperthermophile Thermotoga maritima. Biochemistry 2001, 40, 13107–13113. [Google Scholar]
- Lee, CF; Allen, MD; Bycroft, M; Wong, KD. Electrostatic interactions contribute reduced heat capacity change of unfolding in a thermophilic ribosomal protein L30e. J. Mol. Biol 2005, 348, 419–431. [Google Scholar]
- Razvi, A; Scholtz, JM. A thermodynamic comparison of HPr proteins from extremophilic organisms. Biochemistry 2006, 45, 4084–4092. [Google Scholar]
- Gianni, S; Ivarsson, Y; Jemth, P; Brunori, M; Travaglini-Allocatelli, C. Identification and characterization of protein folding intermediates. Biophys. Chem 2007, 128, 105–113. [Google Scholar]
- Mukaiyama, A; Haruki, M; Ota, M; Koga, Y; Takano, K; Kanaya, S. A hyperthermophilic protein acquires function at the cost of stability. Biochemistry 2006, 45, 12673–12679. [Google Scholar]
- Dong, H; Mukaiyama, A; Tadokoro, T; Koga, Y; Takano, K; Kanaya, S. Hydrophobic effect on the stability and folding of a hyperthermophilic protein. J. Mol. Biol 2008, 378, 264–272. [Google Scholar]
- Takano, K; Higashi, R; Okada, J; Mukaiyama, A; Tadokoro, T; Koga, Y; Kanaya, S. Proline effect on the thermostability and slow unfolding of a hyperthermophilic protein. J. Biochem 2009, 145, 79–85. [Google Scholar]
- Mukaiyama, A; Koga, Y; Takano, K; Kanaya, S. Osmolyte effect on the stability and folding of a hyperthermophilic protein. Proteins: Struct., Funct., Bioinf 2008, 71, 110–118. [Google Scholar]
- Crouch, RJ, Dirksen, ML, Ribonuclease, H, Linn, SM, Robert, RJ, Eds.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, USA, 1982; pp. 211–241.
- Ohtani, N; Haruki, M; Morikawa, M; Kanaya, S. Molecular diversities of RNase H. J. Biosci. Bioeng 1999, 88, 12–19. [Google Scholar]
- Muroya, A; Tsuchiya, D; Ishikawa, M; Haruki, M; Morikawa, M; Kanaya, S; Morikawa, K. catalytic center of an archaeal type2 ribonuclease H as revealed by X-ray crystallographic and mutational analyses. Protein Sci 2001, 10, 707–714. [Google Scholar]
- Takano, K; Endo, S; Mukaiyama, A; Chon, H; Matsumura, H; Koga, Y; Kanaya, S. Structure of amyloid beta fragments in aqueous environments. FEBS J 2006, 273, 150–158. [Google Scholar]
- Takano, K; Katagiri, Y; Mukaiyama, A; Chon, H; Matsumura, H; Koga, Y; Kanaya, S. Conformational contagion in a protein: structural properties of a chameleon sequence. Proteins 2007, 68, 617–625. [Google Scholar]
- Hollien, J; Marqusee, S. A thermodynamic comparison of mesophilic and thermophilic ribonucleases H. Biochemistry 1999, 38, 3831–3836. [Google Scholar]
- Tanford, C. Protein folding. Part C. Adv. Protein Chem 1970, 24, 1–95. [Google Scholar]
- Raschke, TM; Kho, J; Marqusee, S. Confirmation of the hierarchical folding of RNase H: A protein engineering study. Nature Struct. Biol 1999, 6, 825–831. [Google Scholar]
- Hollien, J; Marqusee, S. Comparison of the folding processes of T. thermophilus and E. coli ribonucleases H. J. Mol. Biol 2002, 316, 327–340. [Google Scholar]
- Perl, D; Welker, C; Schindler, T; Schröder, K; Marahiel, MA; Janicke, R; Schmid, FX. Conservation of rapid two-state folding in mesophilic, thermophilic and hyperthermophilic cold shock proteins. Nature Struct. Biol 1998, 5, 229–235. [Google Scholar]
- Dominy, BN; Perl, D; Schmid, FX; Brooks, CL, III. The effect of ionic strength on protein stability: The cold shock protein family. J. Mol. Biol 2002, 319, 541–554. [Google Scholar]
- Schuler, B; Kremer, W; Kalbitzer, HR; Jaenicke, R. Role of entropy in protein thermostability: folding kinetics of a hyperthermophilic cold shock protein at high temperatures using 19F NMR. Biochemistry 2002, 41, 11670–11680. [Google Scholar]
- Wallgren, M; Åden, J; Pylypenko, O; Mikaelsson, T; Johansson, LB-Å; Rak, A; Wolf-Watz, M. Extreme temperature tolerance of a hyperthermophilic protein coupled to residual structure in the unfolded state. J. Mol. Biol 2008, 379, 845–858. [Google Scholar]
- Pace, CN. Contribution of the hydrophobic effect to globular protein stability. J. Mol. Biol 1992, 226, 29–35. [Google Scholar]
- Takano, K; Ogasahara, K; Kaneda, H; Yamagata, Y; Fujii, S; Kanaya, E; Kikuchi, M; Oobatake, M; Yutani, K. Contribution of hydrophobic residues to the stability of human lysozyme: calorimetric studies and X-ray structural analyses of the five isoleucine to valine mutans. J. Mol. Biol 1995, 254, 62–76. [Google Scholar]
- Takano, K; Yamagata, Y; Fujii, S; Yutani, K. Contribution of hydrophobic effect to the stability of human lysozyme: calorimetric studies and X-ray structural analysis of the nine valine to alanine mutans. Biochemistry 1997, 36, 688–698. [Google Scholar]
- Takano, K; Funahashi, J; Yamagata, Y; Fujii, S; Yutani, K. Contribution of water molecules in the interior of a protein to the conformational stability. J. Mol. Biol 1997, 274, 132–142. [Google Scholar]
- Takano, K; Yamagata, Y; Yutani, K. A general rule for the relationship between hydrophobic effect and conformational stability of a protein: Stability and structure of a series of hydrophobic mutants of human lysozyme. J. Mol. Biol 1998, 280, 749–761. [Google Scholar]
- Matthews, BW; Nicholson, H; Beckel, WJ. Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding. Proc. Natl. Acad. Sci. USA 1987, 84, 6663–6667. [Google Scholar]
- Melchionna, S; Sinibaldi, R; Briganti, G. Explanation of the stability of thermophilic proteins based on unique micromorphology. Biophys. J 2006, 90, 4204–4212. [Google Scholar]
- Sterpone, F; Bertonati, C; Briganti, G; Melchionna, S. Key role of proximal water in regulating thermostable proteins. J. Phys. Chem. B 2009, 113, 131–137. [Google Scholar]
- Watanabe, K; Suzuki, Y. Protein thermostabilization by proline substitutions. J. Mol. Catal. B Enzyme 1998, 4, 167–180. [Google Scholar]
- Richardson, JS; Richardson, DC. Amino acid preferences for specific locations at the ends of alpha helices. Science 1988, 240, 1648–1652. [Google Scholar]
- Yancey, PH; Somero, GN. Counteraction of urea destabilization of protein structure by methylamine osmoregulatory compounds of elasmobranch fishes. Biochem. J 1979, 18, 317–323. [Google Scholar]
- Yancey, PH; Clark, ME; Hand, SC; Bowlus, RD; Somero, GN. Living with water stress: Evolution of osmolyte systems. Science 1982, 217, 1214–1222. [Google Scholar]
- Frye, KJ; Royer, CA. The kinetic basis for the stabilization of staphylococcal nuclease by xylose. Protein Sci 1997, 6, 789–793. [Google Scholar]
- Russo, AT; Rösgen, J; Bolen, DW. Osmolyte effects on kinetics of FKBP12 C22A folding coupled with prolyl isomerization. J. Mol. Biol 2003, 330, 851–866. [Google Scholar]
- Atomi, H; Fukui, T; Kanai, T; Morikawa, M; Imanaka, T. Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reported as Pyrococcus sp. KOD1. Archaea 2004, 1, 263–267. [Google Scholar]
- Deckert, G; Warren, PV; Gaasterland, T; Young, WG; Lenox, AL; Graham, DE; Overbeek, R; Snead, MA; Keller, M; Aujay, M. The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature 1998, 392, 353–358. [Google Scholar]
- Nelson, KE; Clayton, RA; Gill, SR; Gwinn, ML; Dodson, RJ; Haft, DH; Hickey, EK; Peterson, JD; Nelson, WC; Ketchum, KA. Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima. Nature 1999, 399, 323–329. [Google Scholar]
- Delong, EF. A phylogenetic perspective on hyperthermophilic microorganisms. Methods Enzymol 2001, 330, 3–11. [Google Scholar]
- Huber, R; Stetter, KO. Discovery of hyperthermophilic miceoorganisms. Methods Enzymol 2001, 330, 11–24. [Google Scholar]
- Berezovsky, IN; Shakhnovich, EI. Physics and evolution of thermophilic adaptation. Proc. Natl. Acad. Sci. USA 2005, 102, 12742–12747. [Google Scholar]
|Table 1. Thermodynamic and kinetic parameters characterizing the stability and refolding/unfolding of proteins from hyperthermophiles.|
|Protein||Organism||G(H2O) (kJ mol−1)||kref(H2O) (s−1)||kref(H2O) (s−1)|
|RNase HII||Thermococcus||43.6 (50 °C)||5.0 × 10−8 (50°C)||7.8 × 10−1 (50 °C)|
|kodakaraensis||48.3 (25 °C)||6.0 × 10−10 (25 °C)||0.4 × 10−1 (25 °C)|
|Cold shock protein||Thermotoga maritima||26.2 (25 °C)||1.8 × 10−2 (25 °C)||5.7 × 102 (25 °C)|
|Ribosomal protein S16||Aquifex aeolicus||24.8 (25 °C)||1.6 × 10−2 (25 °C)||3.8 × 10 (25 °C)|
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/). This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).