- freely available
Int. J. Mol. Sci. 2009, 10(3), 1314-1345; doi:10.3390/ijms10031314
Published: 19 March 2009
Abstract: This review summarizes experimental data illuminating the mechanism of suppression of heat-induced protein aggregation by α-crystallin, one of the small heat shock proteins. The dynamic light scattering data show that the initial stage of thermal aggregation of proteins is the formation of the initial aggregates involving hundreds of molecules of the denatured protein. Further sticking of the starting aggregates proceeds in a regime of diffusion-limited cluster-cluster aggregation. The protective effect of α-crystallin is due to transition of the aggregation process to the regime of reaction-limited cluster-cluster aggregation, wherein the sticking probability for the colliding particles becomes lower than unity.
α-Crystallin, the major protein of the mammalian eye lens, is present therein as a polydisperse heterooligomer of αA- and αB-crystallins, each of a molecular weight of about 20 kDa . In addition to its structural role in the lens, α-crystallin, as a member of the small heat shock protein (sHSP) family, performs the role of a chaperone and prevents aggregation and precipitation of β- and γ- crystallins (including their self-aggregation) [2–6]. Besides the lens, the αB subunit is expressed extensively in many other tissues, including the brain, lungs, spleen, cardiac and skeletal muscles [7–10], where it functions as a chaperone by interacting with several partially folded target proteins. In vitro, α-crystallin (αB- and αA-crystallins) suppresses formation and precipitation of disordered (amorphous) [2,6,11–14], as well as ordered protein aggregates (amyloid fibrils) [15–17].
The quaternary structure of α-crystallin depends on external conditions such as pH, temperature, ionic strength, concentration; the number of its subunits in each assemblage may vary from 15 to 50 [18–22]. Aquilina et al.  have revealed a distribution, primarily of oligomers containing 24–33 subunits, with the dominant species composed of 28 subunits. Additionally, low levels of oligomers, as small as 10-mers and as large as 40-mers, were observed in αB-crystallin by mass spectrometry. Because of the polydispersity of α-crystallin, the true molecular mass and size of the isolated α-crystallin have not yet been determined and molecular mass values from 300 to 12,000 kDa are variously reported in the literature [24–26]. According to several studies, the quaternary assembly of α-crystallin is characterized by a relatively fast subunit-exchange under physiological conditions, indicating a certain degree of conformational flexibility of the α-crystallin protomers [21,26,27]. This proceeds by monomer exchange among individual oligomers, which is temperature-dependent [21,28]. Heating of α-crystallin at 60 °C results in partial unfolding of the protein, which then doubles in molecular weight and increases in size [28–30]. Evans et al.  showed that ordered secondary structure of α-crystallin increases with elevated temperatures up to 60 °C, and is rapidly lost at temperatures of 70 °C and above. According to Aquilina et al. , the rate of subunit exchange is not the critical parameter for chaperone activity of mammalian α-crystallin, in contrast to mechanisms established for analogous proteins from plants, yeast and bacteria.
It is interesting that α-crystallin, like the β- and γ-crystallins, is also able to form fibrils [16,32,33]. Thus, even αA- and αB-crystallins, which are highly stable proteins (like other crystallins), are prone to aggregate and form fibrils, despite their role in protein stabilization in the lens.
α-Crystallin can recognize and bind unfolded proteins over a range of temperatures, and a direct correlation between temperature, monomer exchange and the ability of α-crystallin to suppress amorphous aggregates formation has been demonstrated in a number of studies [11,28,34,35]. Devlin et al.  showed that α-crystallin specifically inhibits nucleation-dependent aggregation of proteins. This ability has been demonstrated by suppression of aggregation of serine proteinase inhibitors by α-crystallin at 50 °C.
The hydrophobic interaction of α-crystallin with unfolded proteins plays a major role in the recognition of the target protein by the chaperone [34,37,38]. The structural changes of α-crystallin in response to increased temperature support a possible “activation” step (an irreversible increase in ordered secondary structure, associated with assembly into the high-molecular-mass form) in the chaperone mechanism [6,28]. This form of the chaperone associates with partially folded βB2-crystallin  and α-lactalbumin  intermediates prior to protein aggregation. Abgar et al.  concluded that interaction of α-crystallin with the destabilized protein is a two-step process. First the destabilized proteins are bound by α-crystallin so that non-specific aggregation of the destabilized protein is prevented. This complex is unstable, and a reorganization and inter-particle exchange of the peptides result in stable and soluble large particles.
α-Crystallin was found to prevent formation of amyloid fibrils by various proteins (e.g. amyloid β-peptide, apolipoprotein C-II, α-synuclein, κ-casein) [15,41–43]. The mechanism by which αB-crystallin prevents growth of amyloid fibrils differs from the known mechanism by which it prevents the amorphous aggregation of proteins [42,43]. It has been shown that α-crystallin inhibits formation of amyloid fibrils by Aβ-(1–40) and apolipoprotein C-II due to interaction of the chaperone with the fibril nucleus, and does not inhibit the relatively rapid fibril elongation upon nucleation [42,43]. αB-Crystallin also prevents the fibril growth of β2-microglobulin under acidic conditions .
The fibril-suppressing ability of αB-crystallin is temperature dependent, and the nature of the aggregating species with which it interacts is also important . The efficiency of α-crystallin in suppressing fibril formation by κ-casein and α-synuclein increases with temperature. This is consistent with an increased chaperone ability of α-crystallin at higher temperatures to protect target proteins from amorphous aggregation . It has been shown that αB-crystallin interrupted α-synuclein aggregation at its earliest stages, most likely by binding to partially folded monomers and thereby preventing their aggregation into fibrillar structures .
The mechanism of interaction of α-crystallin with target proteins has been studied intensively, but details of the mechanism by which α-crystallin accomplishes its chaperone action have not been elucidated.
In the present review a new mechanism of thermal aggregation of globular oligomeric proteins is discussed. According to this mechanism the initial stage of protein aggregation is the stage of formation of the initial aggregates. The starting aggregates contain hundreds of denatured protein molecules. Further growth of protein aggregates is accomplished as a result of the sticking of the starting aggregates or the aggregates of higher order. This stage of protein aggregation proceeds in the diffusion-limited cluster-cluster aggregation (DLCA) regime. This regime implies that each collision results in the sticking of the interacting particles. In the frame of this mechanism of protein aggregation the protective action of α-crystallin is due to diminution of the size of the start aggregates and transition of the aggregation process from the DLCA regime to the reaction-limited cluster-cluster aggregation (RLCA) regime wherein the sticking probability for the colliding particles becomes lower than unity.
2. Mechanisms of Protein Aggregation
One of the earliest studies of protein aggregation was that by London et al. , who revealed an intermediate of the enzyme tryptophanase that aggregated after exposure to 8 M urea. More recently, the idea that partially folded intermediates might be responsible for protein aggregation has evolved [45–54]. Native proteins can unfold under the action of denaturing agents such as guanidine hydrochloride or urea, as well as due to thermal denaturation. Inappropriate exposure of hydrophobic sequences of unfolded proteins leads to formation of non-native conformations, which can interact with existing aggregates . The aggregates can be either amorphous structures, such as inclusion bodies [49,56–58], or ordered fibrils such as amyloid plaques and prion particles [55,59,60].
A distinctive characteristic of the kinetic curves of protein aggregation is the existence of an initial lag period. To explain such a peculiarity of protein aggregation, some models including a nucleation stage were proposed. These models are based on the nucleation-dependent polymerization model developed by Oosawa and Kasai . The reversible association of proteins involves the stage of formation of a nucleus, further growth of the associate proceeding by attachment of monomer to the growing associate. By analogy with the nucleation-dependent polymerization model, a nucleation-dependent aggregation model explaining the peculiarities of the kinetics of irreversible aggregation of proteins has been proposed [62–69]. The model of nucleation-dependent aggregation can be formulated as follows. The first step is unfolding of the protein molecule:where N and U are the native and unfolded states of the protein molecule. Association of several molecules U results in formation of a nucleus: where Un is a nucleus consisting of n monomers U. The stage of aggregate growth is expressed in the attachment of monomer U to the nucleus or aggregates of higher order: When all monomer U is exhausted, the aggregate of a limiting size Um, consisting of m monomers U, is formed. Theoretical analysis of the kinetics of nucleation-dependent aggregation has been performed by Kodaka .
Ben-Zvi and Goloubinoff  showed that sub-stoichiometric amounts of one fast co-aggregating protein can significantly increase thermal aggregation kinetics of otherwise soluble, slow-aggregating protein. The ability of aggregates to seed and propagate aggregation of other proteins is consistent with the idea that thermal aggregation of proteins includes the stage of nucleation.
A number of works are devoted to models of nucleation-dependent aggregation describing amyloid fibril formation [71–77]. Generation of oligomeric aggregation nuclei is considered as a key step at the onset of protein aggregation, accounting for the delay times of polymer appearance that are recorded by in vitro protein aggregation experiments. It has been shown that in the course of immunoglobulin light chain aggregation a relatively unfolded, but compact intermediate (characterized by decreased tertiary and secondary structure at pH below 3) leads to rapid formation of amyloid fibrils. The native-like intermediates, observed between pH 4 and 6, with little loss of secondary structure, but with significant tertiary structure changes, predominantly result in formation of amorphous aggregates . Amyloid fibril formation involves the ordered self-assembly of partially folded species, which are critical soluble precursors of fibrils . It is also indicated that fibril elongation involves addition of a partially unfolded intermediate, rather than a protein in the native state . Kumar et al.  have studied amyloid fibril formation by the small protein barstar. Barstar forms a stable soluble oligomer at low pH, which can transform into protofibrils. The absence of a lag phase suggests that aggregation of stable soluble oligomer forms into protofibrils is not nucleation-dependent. The rate of aggregation increases with increasing protein concentration, but reaches the limit at high concentrations. An analysis of the dependence of the apparent rates of protofibril formation indicates that the slowest stage in protofibil formation is lateral association of linear aggregates. Overall, the study indicates that growth in the course of protofibril formation occurs step-wise through progressively larger and larger aggregates, via multiple pathways, and finally through lateral association of critical aggregates.
Since protein aggregates possess a higher light scattering ability than the original molecules, a convenient way to study protein aggregation kinetics is registration of the increment of the light scattering intensity or apparent optical absorbance. Some authors have tried to analyze the kinetic curves of protein aggregation assuming that proportionality between the apparent optical absorbance and the amount of the aggregated protein exists (for example, [65,66,81]). However it is clear that more reliable information on the mechanism of protein aggregation may be obtained using methods that allow sizing the protein aggregates. On-line analysis of the kinetics of protein aggregation can be carried out using dynamic light scattering (DLS). There are numerous investigations of the aggregation kinetics for different proteins, where the size of protein aggregates was estimated by DLS [14,52,80,82–89].
DLS is commonly used to determine the size of nanoparticles in a suspension by measuring the dynamics of the light-scattering intensity fluctuations. Colloidal particles or macromolecules suspended in fluid undergo Brownian motion. This motion causes fluctuations in the local concentration of the particles resulting in local non-homogeneities of the refractive index. This in turn leads to the light scattering phenomenon. The measured time-dependent autocorrelation function G2(τ) of the scattered light is a function of the delay time τ and for a single decay rate can be represented by the following equation:
This function describes light scattering in system of monodisperse spherical non-interacting particles. The time-independent baseline correlation level A is proportional to the square of the time-averaged intensity I. The factor B is called usually as the intercept of the autocorrelation function. This instrument factor is a measure of quality of light scattering conversion, which is possible to define as the relative coherence of the received scattered light. In accordance with definition B ≤ 1, practical value of B within 0.2–0.9 is acceptable for precise measurement of particle size.
The decay rate Γ of the time-dependent correlation function is directly related to the translation diffusion coefficient D of the particles:where q is the modulus of the scattering vector ( ), n is the refractive index of the solvent, λ0 is the wavelength of the incident light in vacuum and θ is the scattering angle.
The mean hydrodynamic radius of the particles, Rh, then can be calculated using the Stokes-Einstein equation:where kB is Boltzmann’s constant, T is the absolute temperature and η is the viscosity of the solvent. The size calculated from Equation (6) is called the hydrodynamic radius. It may be larger than the radius of the bare particles because of possible layers of solvent, surfactant molecules or (for charged particles) adsorbed ions. In most cases these layers add a negligible correction to the size, except for the smallest sizes measurable. Eqs. (4) and (5) are valid for noninteracting spherical particles. If the particles are involved in an aggregation process, they certainly interact. However, these equations are still applicable to monitor the change of the apparent (“effective”) particle size if the characteristic time of aggregation kinetics is much larger than the time of the measurements.
Direct fitting of experimental data by Equation (4) allows the effective average decay rate to be found. However in experimental practice it is important to know the polydispersity of the particles. Usually, light scattering in polydisperse multicomponent samples gives a multiexponential correlation function. The simple approximation with an appropriate multiexponent model is not effective due to serious mathematical complexity for solution of such, so-called, ill-posed problem. A few methods have suggested solving this problem. The most popular and simple is method of cumulants, which is based on cumulant expansion of the correlation function within average decay rate [90,91]. A final cumulant expression, suitable for fitting practice, is:where Γ̅ is the average decay rate, μ2 and μ3 are the second and third cumulants. Cumulants μ2 and μ3 allow the parameters of a polydispersity distribution to be calculated: polydispersity index PI = μ2/Γ̅2 characterizing the dimensionless width of the polydispersity distribution (see ISO standard ) and asymmetry index (skewness) AI = μ2/μ3; Γ̅ characterizing the dimensionless asymmetry of the polydispersity distribution. Unfortunately, the most mathematically-rigorous method of cumulants can be used only for the unimodal relatively narrow particle size distributions. More universal method of the DLS data processing is based on the regularized Laplace inversion. This method allows finding polymodal particle size distributions with satisfactory reliability of fitting. There are a few such programs. The most popular CONTIN software was developed by S.W. Provencher many years ago. A new version of the regulation software DYNALS has been developed by A. Goldin (Alango, Israel; http://www.photocor.com/dynals.htm). The DYNALS package is well-proven and used by many researchers.
Features of the Photocor Complex DLS setup (Photocor Instruments Inc., USA) are typical in multipurpose installations for studying both static (intensity) and dynamic (time-dependent correlation function) light scattering . The optical bench and precision goniometer are based on a massive base. An He-Ne laser (Coherent, USA, Model 31-2082, 632.8 nm, 10 mW) and the focusing system are fixed on the bench. The thermostat and cell holder are coaxial with the goniometer axis. The temperature is controlled by the PID temperature controller Photocor-TC to within ±0.1 °C. The receiving system (photon counting system Photocor-PC2) is located on the rotating goniometer arm. The photon counting system consists of a set of pinholes for selection of aperture, a low-noise photomultiplier tube (PMT) and an amplifier-discriminator. To avoid the afterpulsing distortions the quasi-cross correlation scheme with two PMTs is used. The output signal produced by the photon counting system occurs as a sequence of standard pulses corresponding to escaped photoelectrons. The raw signal from the photon counting system is analyzed by a single-board correlator Photocor-FC (http://www.photocor.com), which is plugged into a personal computer. The correlator is a multichannel device that performs real-time data processing and accumulation of the correlation function of the light scattering fluctuations. The correlator Photocor-FC has two time-scale modes of operation, i.e., a linear mode with equidistant points of the measured correlation function and a so called multiple-tau mode with the logarithmic order of the correlation points in time. Operation of the correlator in multiple-tau mode needs no tuning of time-scale and is suitable for measurements of multi-peak particle distributions. The linear scale of the correlator enables one to achieve maximum accuracy for measurements of a mono-modal narrow distribution. These cross-correlation system and fast correlator allow measuring particle size in very wide range of 1 nm to 5 μm. The personal computer performs data analysis and instrument control.
Consider, as an example, the kinetics of thermal aggregation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle. Figure 1 shows the dependences of the light scattering intensity on time for GAPDH aggregation at 55 °C. These dependences were obtained at various concentrations of the protein in the interval from 0.05 to 0.4 mg/mL. Diminution of the light scattering intensity at rather high values of time is due to precipitation of large-sized protein aggregates.
According to the DLS data, the distribution of protein aggregates by size is unimodal, with the position of a maximum shifted towards the higher values of the hydrodynamic radius with increasing incubation time (Figure 2). Of special interest are the dependences of the hydrodynamic radius (Rh) of the protein aggregates on time (Figure 3).
It was important to elucidate whether the dependences of Rh on time approached the limiting value at high values of time. The analysis carried out for thermal aggregation of different proteins [14,94–97] showed that above a definite point of time (t > t *) the dependences of Rh on time followed the power law:where is the value of Rh at t = t*, K2 is a constant and df is the fractal dimension of the protein aggregates. The fractal dimension is a structural characteristic of aggregates which are formed as a result of unordered interactions (random aggregation). The mass of an aggregate (M) formed in such a way is connected with its effective radius (R) by the following relationship: M ~ Rdf. The value of df for GAPDH aggregation (Figure 3A) was found to be 1.79 ± 0.05. Such dependences of Rh on time coincide with the DLCA regime of aggregation [98–102]. Under this kinetic regime of aggregation each collision of protein clusters results in their sticking. The DLCA regime is characterized by the universal value of df (df ≈ 1.8) [98,103].
It is evidenced by Figure 3B that the initial parts of the dependences of the hydrodynamic radius of protein aggregates on time are linear. It should be noted that such dependences of hydrodynamic radius on time (initial linear parts followed by the parts described by power law) are typical of aggregation of colloids proceeding in the DLCA regime (see, for example, . The linear part of the dependence of Rh on time can be described by the following equation :where Rh,0 is the hydrodynamic radius of the start aggregates, t0 is the point of time when the start aggregates appear, and t2R is the time interval over which the hydrodynamic radius of the protein aggregates increases from Rh,0 to 2Rh,0. Parameter t2R characterizes the rate of aggregation. The higher the 1/t2R value, the higher is the aggregation rate. Knowing the Rh,0 value, we can calculate parameters t0 and t2R. In the case of thermal aggregation of GAPDH (Figure 3B) the increase in protein concentration resulted in diminution of the lag period characterized by parameter t0 and the increase in the aggregation rate characterized by the 1/t2R value.
Analysis of the light scattering intensity versus the hydrodynamic radius of the protein aggregates plots lead to an important conclusion about the mechanism of protein thermal aggregation.
As is seen from Figure 4, the protein aggregates of rather large size are present in the system at the initial moment of the increment of the light scattering intensity (I). These initial aggregates were called “starting aggregates” . The hydrodynamic radius of the starting aggregates (Rh,0) corresponds to the length cut off on the abscissa axis by the linear dependence of I on Rh. Rh,0 remained practically constant at variation of the GAPDH concentration in the interval from 0.05 to 0.4 mg/mL and was found to be 22 ± 1nm (Figure 4).
In the framework of the proposed mechanism of protein aggregation it becomes clear why the dependences of the light scattering intensity on time tend to level off at rather high values of time. The light scattering ability of the protein aggregate increases with the increase in the aggregate size. However diminution of particle concentration takes place because of their sticking. As a result we observe an apparent leveling off for the light scattering intensity versus time plots. It is significant that this “leveling off does not indicate the completion of the aggregation process. If we measure the size of protein aggregates on the “plateau”, we shall find that the growth of aggregates continues.
Thermal aggregation of aspartate aminotransferase from pig heart mitochondria (mAAT) may serve as another example demonstrating the importance of the light scattering intensity versus the hydrodynamic radius plots and supporting the idea of the start aggregate formation as an initial stage of protein aggregation . Figure 5 shows the dependences of the light scattering intensity on time for mAAT aggregation at 60 °C. The dependences were obtained at various concentrations of mAAT in the interval from 0.05 to 0.4 mg/mL.
The ascending part of the dependence of the light scattering intensity (I) on time (before the precipitation of large-sized aggregates) obeys the exponential law :where Ilim is the limiting value of I at t → ∞ and kI is the rate constant of the first order. The upper abscissa axis (Figure 5) shows the portion of the denatured protein (γden) calculated from the data of differential scanning calorimetry (DSC). As has been shown by Golub et al. , the portion of the denatured protein is identical to the portion of the aggregated protein calculated from measurements of the optical absorbance of the heated protein solution after 30 min centrifugation at 20,000 g. The analysis of the data presented in Figure 5 using Equation (10) shows that the increment of the light scattering intensity is terminated even before the portion of the denatured protein reaches 22% .
mAAT aggregation is accompanied by the growth of protein aggregates (Figure 6). As in the case of GAPDH thermal aggregation, the dependences of Rh on time involve the initial linear parts followed by the parts which are described by power law (Equation 8) with df = 1.82 ± 0.02. The value of df (the fractal dimension of the aggregates) indicates that mAAT aggregation proceeds in the DLCA regime.
The width of the size distribution of protein aggregates formed in the course of mAAT aggregation may be characterized by the polydispersity index (PI) . The temporal character of the changes in the PI value (Figure 7) shows that two parts of the dependences of Rh on time correspond to different physical processes proceeding in the system. The increase in the Rh value over the initial linear part of the dependence of Rh on time is accompanied by the increase in the PI value. The broadening of the distribution of the protein aggregates by size in this time interval is due to the fact that two processes, namely, formation of new start aggregates and their sticking, proceed simultaneously. At t > t* the prevailing process is the sticking of the protein aggregates accompanied by the decrease in the PI value. The limiting value of PI at t → ∞ was found to be 0.14 ± 0.01. Such a low PI value is typical of aggregation of colloids proceeding in the DLCA regime .
Analysis of the dependence of the hydrodynamic radius of the protein aggregates on time is of considerable importance for understanding the mechanism of protein aggregation. As discussed above, in the case of thermal aggregation of GAPDH and mAAT, dependences of Rh on time follow the power law with df parameter close to 1.8. A similar picture was observed for thermal aggregation of βL-crystallin from bovine eye lens , tobacco mosaic virus coat protein  and glycogen phosphorylase b (Phb) from rabbit skeletal muscle [97,111]. Thus one can assume that thermal aggregation of proteins generally proceeds in the DLCA regime.
Construction of the light scattering intensity versus the hydrodynamic radius plots allowed sizing the start aggregates formed in the course of thermal aggregation of mAAT (Figure 8). The hydrodynamic radius of the start aggregates (Rh,0) may be calculated from the length cut off on the abscissa axis by the linear dependence of I on Rh. The Rh,0 value was found to be 79 ± 3 nm. It should be noted that the hydrodynamic radius of the native dimeric molecule of mAAT with molecular mass of 91.2 kDa under non-denaturing conditions is 4.13 ± 0.18 nm. A rough estimate of the number of unfolded mAAT monomers in the start aggregate gives the value of 1030 ± 100.
The light scattering intensity versus the hydrodynamic radius plots were also used for estimation of the start aggregate size for thermal aggregation of βL-crystallin from bovine eye lens , tobacco mosaic virus coat protein  and Phb .
To obtain more reliable information on the stage of the starting aggregate formation, conditions had to be chosen, whereby the simultaneous registration of the native enzyme and starting aggregates was possible. For this purpose aggregation of GAPDH (1 mg/mL) at 45 °C was studied . The time of accumulation of the autocorrelation function was 30 s. Over first four minutes only the native GAPDH tetramer with Rh = 5.4 ± 0.3 nm was detected (Figure 8A). At t = 5 min the starting aggregates with Rh = 97.0 ± 0.2 nm appeared. Over the interval from 5 to 8 minutes the size of the protein aggregates remained constant. At t > 8 min the size of the protein aggregates began to increase because of the sticking of the starting aggregates (Figure 9B and C and Figure 10). These data demonstrate that formation of the starting aggregates proceeds on the all-or-none principle without accumulation of intermediates.
Sluzky et al.  were the first to observe formation of primary protein aggregates with the hydrodynamic radius of 85 ± 10 nm, when studying insulin aggregation upon shaking at 37 °C. When studying the thermal aggregation of β-lactoglobulin at pH 7.0 by means of size exclusion chromatography, it was shown that there was a clear separation between a narrow peak that corresponded to residual native protein and a broad peak that corresponded to the aggregates [86,115–117]. This observation implied that the aggregates of the minimum size contained many monomers, and that no or very few stable oligomers were formed. In accordance with these data, the authors supposed that the first stage of thermal aggregation of β-lactoglobulin is the step of primary aggregate formation. Further association of primary aggregates gives large self-similar aggregates with fractal dimension df of 1.7 in the absence of salt . Although the primary aggregates postulated in the noted works [86,115–118] are cross-linked by intermolecular disulfide bonds (in pH range 6.4-8.0), such aggregates are evidently similar to the start aggregates we observed for βL-crystallin and other proteins [14,95].
On the basis of the data of Durand et al.  on the sensitivity of sticking of the primary aggregates to variation of ionic strength, one can assume that the starting aggregates may be sufficiently stable at low ionic strength values. From this point of view it is of interest to discuss the properties of the so-called inactivated actin. Turoverov et al.  observed that 60 min incubation of rabbit skeletal muscle actin (5 mM Tris-HCl, pH 8.2) at 60 °C resulted in the formation of relatively stable particles with average sedimentation coefficient of 20 S (inactivated actin). The size of particles of inactivated actin was independent of the protein concentration (within the limits 0.05–1.0 mg/mL). A dramatic increase in 1-anilinonaphthalene-8-sulfonate binding to inactivated actin, in comparison with native and unfolded protein, indicated that the inactivated actin had solvent-exposed hydrophobic clusters on the surface. In our opinion inactivated actin may be considered as a “frozen” starting aggregate.
Thus, investigation of the kinetics of thermal aggregation of proteins by DLS allowed proposing the mechanism of protein aggregation demonstrated in Figure 11 [14,95]. The initial stage of the aggregation process is formation of the starting aggregates. Further sticking of the starting aggregates and aggregates of higher order proceeds in the DLCA regime.
3. Mechanism of Protein Aggregation in the Presence of α-crystallin
Suppression of thermal aggregation of proteins by α-crystallin is a well-studied phenomenon [2,3,11,28,34,35]. Figure 12 shows the effect of α-crystallin on aggregation of mAAT at 60 °C . The addition of α-crystallin results in a lower increment of the light scattering intensity in time.
DLS made it possible to characterize the change in the distribution of the protein aggregates by size in the course of mAAT aggregation in the presence of α-crystallin . At low times of incubation the distribution of protein aggregates by size was unimodal (Figure 13A and B; incubation times were 100 and 200 min). However, the splitting of protein aggregate population into two components took place in the course of the aggregation process (Figure 13C and D; incubation times were 300 and 350 min).
It should be noted that bimodal character of distribution of the protein aggregates at rather high values of the incubation time was also observed for thermal aggregation of βL-crystallin from bovine eye lens  and GAPDH from rabbit skeletal muscle .
The distinctive property of the dependences of the protein aggregate hydrodynamic radius on time for mAAT aggregation in the presence of α-crystallin is that the initial parts of these dependences follow the exponential law:where Rh,0 is the hydrodynamic radius of the initial particles participating in the aggregation process, t0 is the duration of the lag period for the aggregation process and t2R is the time interval over which the Rh value of aggregates is doubled. Parameter t2R characterizes the rate of aggregation. The higher 1/t2R value, the higher is the rate of aggregation. If parameter Rh,0 is known, parameter t0 may be calculated using Equation (11). The solid curves in the insets in Figure 14A-C are calculated from Equation (11). The 1/t2R value decreased from 1.0 to 0.07 min−1, when the concentration of α-crystallin increased from 0.15 to 0.4 mg/mL.
The exponential character of the dependence of the hydrodynamic radius of the protein aggregates on time indicates that the aggregation process proceeds in the regime of reaction-limited cluster-cluster aggregation (RLCA) [98,101,108,120]. Fulfillment of the RLCA regime means that the sticking probability for the colliding particles is lower than unity. It should be noted that the exponential character of the dependences of the hydrodynamic radius of the protein aggregates on time was also observed for thermal aggregation of βL-crystallin , GAPDH  and Phb  studied in the presence of α-crystallin.
Figure 14 shows the dependences of the hydrodynamic radius of the protein aggregates on time obtained for aggregation of mAAT (0.2 mg/mL) in the presence of various concentrations of α-crystallin in the interval from 0.1 to 0.4 mg/mL . As is evident from this Figure, a clearly expressed splitting of the protein aggregate population into two components at rather high values of incubation time was observed at the α-crystallin concentrations of 0.1, 0.15 and 0.2 mg/mL (panels A, B and C). The point of time when such a splitting occurred was designated as tcrit . At α-crystallin concentration of 0.4 mg/mL, the growth of protein aggregates was strongly suppressed.
It should be noted that bimodal character of distribution of the protein aggregates at rather high values of the incubation time was also observed for thermal aggregation of βL-crystallin from bovine eye lens  and GAPDH from rabbit skeletal muscle .
The light scattering intensity versus the hydrodynamic radius plots may be used for estimation of the hydrodynamic radius of the initial particles (Rh,0) participating in the aggregation process. As it is demonstrated in Figure 15, the Rh,0 values measured for mAAT aggregation in the presence of α-crystallin at concentrations of 0.1, 0.15 and 0.2 mg/mL were practically identical. The average value of the Rh,0 was found to be 17.7 ± 1.3 nm. It should be noted that this Rh,0 value exceeds the Rh,0 value for α-crystallin under non-aggregating conditions (Rh = 11.0 ± 0.1 nm) . The hydrodynamic radius of the start aggregates was found to be 79 ± 3 nm in the case of thermal aggregation of mAAT in the absence of α-crystallin. Thus drastic decrease in the size of the start aggregates takes place in the presence of α-crystallin. An analogous picture was observed when studying the effect of α-crystallin on thermal aggregation of βL-crystallin , GAPDH  and Phb .
The reason of the splitting of the protein aggregate population into two components in the presence of α-crystallin may be the following. When the process of protein aggregation is studied in the absence of the chaperone, aggregation proceeds in the DLCA regime. Fulfillment of this regime means that each collision of the interacting particles results in their sticking. The high sticking probability is ensured by the high density of sticking sites (the sites of aggregation) on the surface of a starting aggregate. When aggregation of the protein substrate proceeds in the presence of α-crystallin, the starting aggregates containing incorporated α-crystallin are formed. Such starting aggregates are characterized by a lower density of the aggregation sites on the aggregate surface. As a result, the sticking probability for such colliding particles becomes lower than unity. Consider the sticking of particles with a limited number of the aggregation sites on the particle surface. The sticking of two particles results in disappearance of the aggregation sites in the contact region because of their mutual screening. Thus the growth of the protein aggregate may bring about formation of aggregates in which the number of the aggregation sites on the aggregate surface is greatly reduced. Such aggregates correspond to the component with lower values of Rh in the aggregate population (solid circles in Figure 14A–C at t > tcrit). From this point of view the component with higher values of Rh (hollow circles in Figure 14A–C at t > tcrit) corresponds to the component that retains relatively high density of the aggregation sites on the aggregate surface .
The study of the effect of α-crystallin on thermal aggregation of proteins by DLS shows that the protective action of α-crystallin is due to diminution of the size of the start aggregates, transition of the aggregation process from the DLCA regime to the RLCA regime and, consequently, the decrease in sticking probability for the colliding particles. At rather high concentrations of α-crystallin, for example, at concentration of 0.4 mg/mL for the conditions used in the experiments shown in Figure 14, α-crystallin -unfolded protein complexes are formed, which are incapable of aggregating. The overall mechanism of the protective action of α-crystallin is represented in Figure 16 [14,95].
Since the addition of α-crystallin results in the transition of the aggregation process for the protein substrate from the DLCA regime to the RLCA regime, one can expect that aggregation of α-crystallin itself will proceed in the RLCA regime. Actually Andreasi Bassi et al.  showed that the dependence of the hydrodynamic radius on time for the protein aggregates formed in the course of aggregation of α-crystallin at 55 °C in the presence of 16 mM CaCl2 (10 mM Tris-HCl buffer, pH 7.4) followed the exponential law.
4. Effect of α-Crystallin on Thermal Stability of Proteins
In this review we have discussed the effect of α-crystallin on heat-induced aggregation of proteins. Since the initial stage of thermal aggregation of proteins is unfolding of the protein molecule, it is of interest to discuss the effect of α-crystallin on protein stability. The analysis of literature data shows that α-crystallin usually does not affect thermal stability of the proteins or, in some instances, stabilizes the proteins. For example, it was shown that α-crystallin interfered with heat-induced inactivation of pig heart citrate synthase, cytosolic malate dehydrogenase , catalase [122,123], sorbitol dehydrogenase from sheep liver , the restriction enzyme Nde I cleaving plasmid DNA  and Chlamydia pneumoniae uracil DNA glycosylase . Rajaraman et al.  have concluded that interaction of α-crystallin with early unfolded protein intermediates reduces the partitioning into aggregation-prone intermediates.
It is surprising that in some cases the decrease of the protein stability in the presence of α-crystallin is observed. Khanova et al.  have studied the effect of α-crystallin on thermal stability of GAPDH using DSC (Figure 17). Thermal unfolding of GAPDH (0.4 mg/mL) is characterized by sharp thermal transition with a maximum at 61.6 °C (curve 1). In the presence of α-crystallin the shift of the maximum position (Tmax) towards lower temperatures is observed. The shift is dependent on concentration of α-crystallin. When concentration of α-crystallin is 0.4 mg/mL the value of Tmax decreases to 58.0 °C. These data indicate that stability of GAPDH is markedly reduced in the presence of α-crystallin. The displacement of the maximum position on the DSC profiles of GAPDH towards lower temperatures in the presence of α-crystallin suggests that α-crystallin interacts with the heating-induced intermediates of GAPDH unfolding. The products of heat-induced dissociation of the tetrameric form of GAPDH, namely the dimeric and monomeric forms, may play a role of such unfolding intermediates . It should also be noted that α-crystallin accelerates thermal inactivation of GAPDH at 45 °C (K.A. Markossian, unpublished data). The analogous picture has been observed, when studying the effect of α-crystallin on thermal inactivation and denaturation of mAAT .
The kinetics of thermal inactivation of mAAT (0.1 mg/mL) at 65 °C are shown in Figure 18. This Figure demonstrates the time-course of the decline of the enzymatic activity of mAAT. The time of half-conversion (t0.5) was found to be 32.7 ± 0.4 min (curve 1). The addition of α-crystallin results in acceleration of the inactivation process. When the concentration of α-crystallin is equal to 0.05 mg/mL, the t0.5 value decreases to 18.0 ± 0.6 min (curve 2). At the α-crystallin concentration of 0.1 mg/mL the t0.5 value was found to be 6.3 ± 0.3 min (curve 3).
The effect of α-crystallin on the thermostability of mAAT characterized by DSC is shown in Figure 19 . The DSC profile for mAAT (1 mg/mL) has a maximum (Tmax) of 72.4 °C (curve 1). The position of a maximum of the DSC profile for α-crystallin (1 mg/mL) corresponds to 63.7 °C (curve 2). The complex shape of the DSC profile for the mixture of mAAT and α-crystallin (curve 3) is due to non-homogeneity of the sample. It is evident that the shoulder at 63.7 °C corresponds to α-crystallin. The Tmax value for the DSC profile under discussion was found to be 70.9 °C. One can assume that such a decrease in the Tmax value is indicative of the reduced stability of mAAT in the presence of α-crystallin. It should be noted that destabilizing effect of α-crystallin has also been demonstrated for Phb .
Scheme 1 below may serve to explain the effect of α-crystallin on thermal denaturation of a protein substrate. This scheme is a modification of the schematic diagram put forward by Rajaraman et al. . In scheme 1 N is the native form of a protein, I1 is the early unfolding intermediate and In is the late aggregation-prone intermediate. I1 ·C and In ·C are, respectively, intermediates I1 and In complexed with α-crystallin. It is assumed that the initial stages of protein unfolding and complexion of α-crystallin with an early unfolding intermediate I1 are reversible. Complexation of α-crystallin with the early unfolding intermediate may explain the influence of α-crystallin on denaturation of a protein substrate. One can expect that intermediate I1, which is formed as a result of partial unfolding of the native protein molecule, possesses a lower thermostability than the native protein. Protein-protein interactions usually lead to stabilization of the resulting complex. Therefore, one can expect that I1 ·C complex is more stable than the I1 form. The resulting stability of I1 ·C complex may exceed the stability of the native protein form N. In this case α-crystallin decreases the rate of denaturation of the protein substrate (or the rate of inactivation if a protein substrate is an enzyme). However if the decrease in the stability during transition from state N to state I1 is sufficiently significant, the resulting stability of the I1 ·C complex may be found to be lower than that for the native molecule. In this case α-crystallin will cause destabilization of a protein substrate or, respectively, acceleration of inactivation of an enzyme used as a protein substrate. Thus, Scheme 1 may explain either stabilizing or destabilizing action of α-crystallin, when studying denaturation of a protein substrate (or inactivation of an enzyme).
One of the functions of molecular chaperones is binding of proteins in unfolded state, which are formed in the course of folding of newly synthesized polypeptide chains or as a result of unfolding of the native proteins under stress conditions. Owing to complexation of unfolded proteins with chaperones, aggregation of proteins in unfolded state is suppressed. The comparative analysis of the anti-aggregation effect of chaperones of different classes is one of the important tasks of the modern biochemistry of chaperones.
An understanding of the mechanism of the anti-aggregation effect of chaperones needs a clarification of the mechanism of protein aggregation. As the DLS studies have shown, the first stage of heat-induced aggregation of proteins is the stage of formation of the starting aggregates. Further growth of the aggregates proceeds as a result of sticking of the starting aggregates and aggregates of higher order. It is evident that the starting aggregates formed in the course of aggregation, resulting in formation of amorphous aggregates, are similar to protofibrils formed in the course of assembly of fibrils.
The anti-aggregation effect of α-crystallin, one of representatives of the family of small heat-shock proteins, is due to diminution of the size of the starting aggregates and decrease in the sticking probability for the colliding particles. A different model may be applicable to chaperones of other classes. For example, chaperonin GroEL, a representative of Hsp60, does not affect the size of the start aggregates, but similarly to α-crystallin, transfers the aggregation process from the diffusion-limited regime to the RLCA regime (K.A. Markossian, unpublished data).
It is significant that the analysis of the dependence of the protein aggregate hydrodynamic radius on time allows revealing proteins that possess their own chaperone-like activity. For example, Markossian et al.  observed that the dependences of hydrodynamic radius on time for thermal aggregation of yeast alcohol dehydrogenase I (ADH) followed the exponential law. Based on this fact, the authors assumed that ADH might reveal chaperone-like activity. Actually the capability of suppressing aggregation of the protein substrate by ADH was demonstrated in the test-system based on aggregation of UV-irradiated βL-crystallin from bovine eye lens . Thus some proteins with well-known biological functions (for example, enzymes) may act as chaperones, enhancing the total chaperone potential of the cell and providing for suppression of unfolded protein aggregation.
This study was funded by the Russian Foundation for Basic Research (grant 08-04-00666-a) and the Program “Molecular and Cell Biology” of the Presidium of the Russian Academy of Sciences.
- Horwitz, J. Alpha-crystallin. Exp. Eye Res 2003, 76, 145–153, doi:10.1016/S0014-4835(02)00278-6. 12565801
- Horwitz, J. Alpha-crystallin can function as a molecular chaperone. Proc. Natl. Acad. Sci. USA 1992, 89, 10449–10453, doi:10.1073/pnas.89.21.10449. 1438232
- Carver, JA; Aquilina, JA; Cooper, PG; Williams, GA; Truscott, RJ. Alpha-crystallin: molecular chaperone and protein surfactant. Biochim. Biophys. Acta 1994, 1204, 195–206, doi:10.1016/0167-4838(94)90009-4. 8142460
- Boyle, D; Takemoto, L. Characterization of the alpha-gamma and alpha-beta complex: evidence for an in vivo functional role of alpha-crystallin as a molecular chaperone. Exp. Eye Res 1994, 58, 9–15, doi:10.1006/exer.1994.1190. 8157104
- Bloemendal, H; de Jong, W; Jaenicke, R; Lubsen, NH; Slingsby, C; Tardieu, A. Ageing and vision: structure, stability and function of lens crystallins. Prog. Biophys. Mol. Biol 2004, 86, 407–485, doi:10.1016/j.pbiomolbio.2003.11.012. 15302206
- Evans, P; Slingsby, C; Wallace, BA. Association of partially folded lens betaB2-crystallins with the alpha-crystallin molecular chaperone. Biochem. J 2008, 409, 691–699, doi:10.1042/BJ20070993. 17937660
- Iwaki, T; Kume-Iwaki, A; Goldman, JE. Cellular distribution of alpha B-crystallin in non-lenticular tissues. J. Histochem. Cytochem 1990, 38, 31–39, doi:10.1177/38.1.2294148. 2294148
- Kato, K; Shinohara, H; Kurobe, N; Inaguma, Y; Shimizu, K; Ohshima, K. Tissue distribution and developmental profiles of immunoreactive alpha B crystallin in the rat determined with a sensitive immunoassay system. Biochim. Biophys. Acta 1991, 1074, 201–208, doi:10.1016/0304-4165(91)90062-L. 2043672
- Srinivasan, AN; Nagineni, CN; Bhat, SP. alpha A-crystallin is expressed in non-ocular tissues. J. Biol Chem 1992, 267, 23337–23341. 1429679
- Horwitz, J. The function of alpha-crystallin in vision. Semin. Cell Dev. Biol 2000, 11, 53–60, doi:10.1006/scdb.1999.0351. 10736264
- Wang, K; Spector, A. The chaperone activity of bovine alpha crystallin. Interaction with other lens crystallins in native and denatured states. J. Biol Chem 1994, 269, 13601–13608. 7909809
- Van Montfort, R; Slingsby, C; Vierling, E. Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones. Adv. Protein Chem 2001, 59, 105–156. 11868270
- Carver, JA; Lindner, RA; Lyon, C; Canet, D; Hernandez, H; Dobson, CM; Redfield, C. The interaction of the molecular chaperone alpha-crystallin with unfolding alpha-lactalbumin: a structural and kinetic spectroscopic study. J. Mol Biol 2002, 318, 815–827, doi:10.1016/S0022-2836(02)00144-4. 12054825
- Markossian, KA; Kurganov, BI; Levitsky, DI; Khanova, HA; Chebotareva, NA; Samoilov, AM; Eronina, TB; Fedurkina, NV; Mitskevich, LG; Merem’yanin, AV; Kleymenov, SY; Makeeva, VF; Muronets, VI; Naletova, IN; Shalova, IN; Asryants, RA; Schmalhausen, EV; Saso, L; Panyukov, YV; Dobrov, EN; Yudin, IK; Timofeeva, AC; Muranov, KO; Ostrovsky, MA. Mechanisms of chaperone-like activity. In Protein Folding: New Research; Obalinsky, TR, Ed.; Nova Science Publishers Inc: New York, 2006; pp. 89–171.
- Rekas, A; Jankova, L; Thorn, DC; Cappai, R; Carver, JA. Monitoring the prevention of amyloid fibril formation by alpha-crystallin. Temperature dependence and the nature of the aggregating species. FEBS J 2007, 274, 6290–6304, doi:10.1111/j.1742-4658.2007.06144.x. 18005258
- Ecroyd, H; Carver, JA. Crystallin proteins and amyloid fibrils. Cell. Mol. Life Sci 2009, 66, 62–81, doi:10.1007/s00018-008-8327-4. 18810322
- Pullara, F; Emanuele, A. Early stages of beta2-microglobulin aggregation and the inhibiting action of alphaB-crystallin. Proteins 2008, 73, 1037–1046, doi:10.1002/prot.22122. 18543331
- Siezen, RJ; Bindels, JG; Hoenders, HJ. The quaternary structure of bovine alpha-crystallin. Effects of variation in alkaline pH, ionic strength, temperature and calcium ion concentration. Eur. J. Biochem 1980, 111, 435–444, doi:10.1111/j.1432-1033.1980.tb04958.x. 7460906
- Koretz, JF; Augusteyn, RC. Electron microscopy of native and reconstituted alpha crystallin aggregates. Curr. Eye Res 1988, 7, 25–30, doi:10.3109/02713688809047016. 3359802
- van den Oetelaar, PJ; van Someren, PF; Thomson, JA; Siezen, RJ; Hoenders, HJ. A dynamic quaternary structure of bovine alpha-crystallin as indicated from intermolecular exchange of subunits. Biochemistry 1990, 29, 3488–3493, doi:10.1021/bi00466a010. 2354148
- Bova, MP; Ding, LL; Horwitz, J; Fung, BK. Subunit exchange of alphaA-crystallin. J. Biol Chem 1997, 272, 29511–29517, doi:10.1074/jbc.272.47.29511. 9368012
- Vanhoudt, J; Aerts, T; Abgar, S; Clauwaert, J. Quaternary structure of bovine alpha-crystallin: influence of temperature. Int. J. Biol Macromol 1998, 22, 229–237, doi:10.1016/S0141-8130(98)00020-8. 9650077
- Aquilina, JA; Benesch, JL; Bateman, OA; Slingsby, C; Robinson, CV. Polydispersity of a mammalian chaperone: mass spectrometry reveals the population of oligomers in alpha B-crystallin. Proc. Natl. Acad. Sci. USA 2003, 100, 10611–10612, doi:10.1073/pnas.1932958100. 12947045
- Thomson, JA; Augusteyn, RC. αm-Crystallin: the native form of the protein? Exp. Eye Res 1983, 37, 367–377, doi:10.1016/0014-4835(83)90173-2. 6641820
- Wang, XW; Bettelheim, FA. Second virial coefficient of alpha-crystallin. Proteins 1989, 5, 166–169, doi:10.1002/prot.340050211. 2748579
- Vanhoudt, J; Abgar, S; Aerts, T; Clauwaert, J. Native quaternary structure of bovine alpha-crystallin. Biochemistry 2000, 39, 4483–4492, doi:10.1021/bi990386u. 10757997
- Abgar, S; Backmann, J; Aerts, T; Vanhoudt, J; Clauwaert, J. The structural differences between bovine lens alphaA- and alphaB-crystallin. Eur. J. Biochem 2000, 267, 5916–5925. 10998051
- Putilina, T; Skouri-Panet, F; Prat, K; Lubsen, NH; Tardieu, A. Subunit exchange demonstrates a differential chaperone activity of calf alpha-crystallin toward beta LOW- and individual gamma-crystallins. J. Biol. Chem 2003, 278, 13747–56, doi:10.1074/jbc.M208157200. 12562766
- Raman, B; Rao, CM. Chaperone-like activity and quaternary structure of alpha-crystallin. J. Biol. Chem 1994, 269, 27264–27268. 7961635
- Das, BK; Liang, JJ; Chakrabarti, B. Heat-induced conformational change and increased chaperone activity of lens alpha-crystallin. Curr. Eye Res 1997, 16, 303–309, doi:10.1076/ceyr.16.4.303.10691. 9134318
- Aquilina, JA; Benesch, JL; Ding, LL; Yaron, O; Horwitz, J; Robinson, CV. Subunit exchange of polydisperse proteins: mass spectrometry reveals consequences of alphaA-crystallin truncation. J. Biol. Chem 2005, 280, 14485–14491, doi:10.1074/jbc.M500135200. 15701626
- Meehan, S; Berry, Y; Luisi, B; Dobson, CM; Carver, JA; MacPhee, CE. Amyloid fibril formation by lens crystallin proteins and its implications for cataract formation. J. Biol. Chem 2004, 279, 3413–3419. 14615485
- Meehan, S; Knowles, TP; Baldwin, AJ; Smith, JF; Squires, AM; Clements, P; Treweek, TM; Ecroyd, H; Tartaglia, GG; Vendruscolo, M; Macphee, CE; Dobson, CM; Carver, JA. Characterisation of amyloid fibril formation by small heat-shock chaperone proteins human alphaA-, alphaB- and R120G alphaB-crystallins. J. Mol. Biol 2007, 372, 470–484, doi:10.1016/j.jmb.2007.06.060. 17662998
- Raman, B; Rao, CM. Chaperone-like activity and temperature-induced structural changes of alpha-crystallin. J. Biol. Chem 1997, 272, 23559–23564, doi:10.1074/jbc.272.38.23559. 9295293
- Reddy, GB; Das, KP; Petrash, JM; Surewicz, WK. Temperature-dependent chaperone activity and structural properties of human alphaA- and alphaB-crystallins. J. Biol. Chem 2000, 275, 4565–4570, doi:10.1074/jbc.275.7.4565. 10671481
- Devlin, GL; Carver, JA; Bottomley, SP. The selective inhibition of serpin aggregation by the molecular chaperone, alpha-crystallin, indicates a nucleation-dependent specificity. J. Biol. Chem 2003, 278, 48644–48650, doi:10.1074/jbc.M308376200. 14500715
- Sharma, KK; Kaur, H; Kumar, GS; Kester, K. Interaction of 1,1′-bi(4-anilino)naphthalene-5,5′-disulfonic acid with alpha-crystallin. J. Biol. Chem 1998, 273, 8965–8970, doi:10.1074/jbc.273.15.8965. 9535881
- Kumar, MS; Kapoor, M; Sinha, S; Reddy, GB. Insights into hydrophobicity and the chaperone-like function of alphaA- and alphaB-crystallins: an isothermal titration calorimetric study. J. Biol. Chem 2005, 280, 21726–21730, doi:10.1074/jbc.M500405200. 15817465
- Lindner, RA; Treweek, TM; Carver, JA. The molecular chaperone alpha-crystallin is in kinetic competition with aggregation to stabilize a monomeric molten-globule form of alpha-lactalbumin. Biochem. J 2001, 354, 79–87, doi:10.1042/0264-6021:3540079. 11171082
- Abgar, S; Vanhoudt, J; Aerts, T; Clauwaert, J. Study of the chaperoning mechanism of bovine lens alpha-crystallin, a member of the alpha-small heat shock superfamily. Biophys. J 2001, 80, 1986–1995, doi:10.1016/S0006-3495(01)76168-1. 11259311
- Stege, GJ; Renkawek, K; Overkamp, PS; Verschuure, P; van Rijk, AF; Reijnen-Aalbers, A; Boelens, WC; Bosman, GJ; de Jong, WW. The molecular chaperone alphaB-crystallin enhances amyloid beta neurotoxicity. Biochem. Biophys. Res. Commun 1999, 262, 152–156, doi:10.1006/bbrc.1999.1167. 10448084
- Hatters, DM; Lindner, RA; Carver, JA; Howlett, GJ. The molecular chaperone, alpha-crystallin, inhibits amyloid formation by apolipoprotein C-II. J. Biol. Chem 2001, 276, 33755–33761, doi:10.1074/jbc.M105285200. 11447233
- Raman, B; Ban, T; Sakai, M; Pasta, SY; Ramakrishna, T; Naiki, H; Goto, Y; Rao, CM. AlphaB-crystallin, a small heat-shock protein, prevents the amyloid fibril growth of an amyloid beta-peptide and beta2-microglobulin. Biochem. J 2005, 392, 573–581, doi:10.1042/BJ20050339. 16053447
- London, J; Skrzynia, C; Goldberg, ME. Renaturation of Escherichia coli tryptophanase after exposure to 8 M urea. Evidence for the existence of nucleation centers. Eur. J. Biochem 1974, 47, 409–415, doi:10.1111/j.1432-1033.1974.tb03707.x. 4607014
- Fink, AL. Protein aggregation: folding aggregates, inclusion bodies and amyloid. Fold. Des 1998, 3, R9–23, doi:10.1016/S1359-0278(98)00002-9. 9502314
- Dobson, CM. Protein misfolding, evolution and disease. Trends Biochem. Sci 1999, 24, 329–332, doi:10.1016/S0968-0004(99)01445-0. 10470028
- Wetzel, R. Mutations and off-pathway aggregation of proteins. Trends Biotechnol 1994, 12, 193–198, doi:10.1016/0167-7799(94)90082-5. 7764903
- Jaenicke, R. Folding and association versus misfolding and aggregation of proteins. Philos. Trans. R. Soc. Lond. B Biol. Sci 1995, 348, 97–105, doi:10.1098/rstb.1995.0050. 7770492
- Kopito, RR. Aggresomes, inclusion bodies and protein aggregation. Trends Cell Biol 2000, 10, 524–530, doi:10.1016/S0962-8924(00)01852-3. 11121744
- Chiti, F; Stefani, M; Taddei, N; Ramponi, G; Dobson, CM. Rationalization of the effects of mutations on peptide and protein aggregation rates. Nature 2003, 424, 805–808, doi:10.1038/nature01891. 12917692
- Alexandrescu, AT. Amyloid accomplices and enforcers. Protein Sci 2005, 14, 1–12. 15576561
- Murphy, RM; Lee, CC. Laser light scattering as an indispensable tool for probing protein aggregation. In Misbehaving Proteins: Protein (mis)Folding, Aggregation, and Stability; Murphy, RM, Tsai, AM, Eds.; Springer: New York, USA, 2006; Volume VIII, pp. 147–165.
- Wetzel, R. Amyloid fibrils-common threads in the natural history of proteins. Acc. Chem. Res 2006, 39, 567, doi:10.1021/ar0681934. 16981671
- Stefani, M. Protein folding and misfolding on surfaces. Int. J. Mol. Sci 2008, 9, 2515–2542, doi:10.3390/ijms9122515. 19330090
- Perutz, MF. Amyloid fibrils. Mutations make enzyme polymerize. Nature 1997, 385, 773–775. 9039907
- Georgiou, G; Valax, P. Expression of correctly folded proteins in Escherichia coli. Curr. Opin. Biotechnol 1996, 7, 190–197, doi:10.1016/S0958-1669(96)80012-7. 8791338
- Villaverde, A; Carrio, MM. Protein aggregation in recombinant bacteria: biological role of inclusion bodies. Biotechnol. Lett 2003, 25, 1385–1395, doi:10.1023/A:1025024104862. 14514038
- Markossian, KA; Kurganov, BI. Protein folding, misfolding, and aggregation. Formation of inclusion bodies and aggresomes. Biochemistry (Mosc) 2004, 69, 971–84, doi:10.1023/B:BIRY.0000043539.07961.4c.
- Serio, TR; Cashikar, AG; Kowal, AS; Sawicki, GJ; Moslehi, JJ; Serpell, L; Arnsdorf, MF; Lindquist, SL. Nucleated conformational conversion and the replication of conformational information by a prion determinant. Science 2000, 289, 1317–1321, doi:10.1126/science.289.5483.1317. 10958771
- Guo, J; Hall, CK; Xu, Y; Wetzel, R. Modeling protein aggregate assembly and structure. In Biological and Medical Physics, Biomedical Engineering; Xu, Y, Xu, D, Liang, J, Eds.; Springer Science+Business Media, LLC: New York, USA, 2007; Volume 1, pp. 279–317.
- Oosawa, F; Kasai, M. A theory of linear and helical aggregations of macromolecules. J. Mol. Biol 1962, 4, 10–21, doi:10.1016/S0022-2836(62)80112-0. 14482095
- Speed, MA; King, J; Wang, DI. Polymerization mechanism of polypeptide chain aggregation. Biotechnol. Bioeng 1997, 54, 333–343, doi:10.1002/(SICI)1097-0290(19970520)54:4<333::AID-BIT6>3.0.CO;2-L. 18634100
- Kurganov, BI; Rafikova, ER; Dobrov, EN. Kinetics of thermal aggregation of tobacco mosaic virus coat protein. Biochemistry (Mosc) 2002, 67, 525–533, doi:10.1023/A:1015589926728.
- Kurganov, BI. Estimation of the activity of molecular chaperones in test-systems based on suppression of protein aggregation. Usp Biol Khim (Mosc) 2002, 42, 89–138.
- Kurganov, BI. Kinetics of protein aggregation. Quantitative estimation of the chaperone-like activity in test-systems based on suppression of protein aggregation. Biochemistry (Mosc) 2002, 67, 409–422, doi:10.1023/A:1015277805345.
- Wang, K; Kurganov, BI. Kinetics of heat- and acidification-induced aggregation of firefly luciferase. Biophys. Chem 2003, 106, 97–109, doi:10.1016/S0301-4622(03)00134-0. 14556900
- Kodaka, M. Requirements for generating sigmoidal time-course aggregation in nucleation-dependent polymerization model. Biophys. Chem 2004, 107, 243–253, doi:10.1016/j.bpc.2003.09.013. 14967239
- Kodaka, M. Interpretation of concentration-dependence in aggregation kinetics. Biophys. Chem 2004, 109, 325–332, doi:10.1016/j.bpc.2003.12.003. 15110949
- Kurganov, BI. Protein aggregation kinetics. In Chemical and Biological Kinetics; Burlakova, EB, Varfolomeev, SD, Eds.; Koninklijke Brill NV: The Netherlands, Leiden, Holand, 2005; Volume 2, pp. 251–279.
- Ben-Zvi, AP; Goloubinoff, P. Proteinaceous infectious behavior in non-pathogenic proteins is controlled by molecular chaperones. J. Biol. Chem 2002, 277, 49422–49427, doi:10.1074/jbc.M209163200. 12377766
- Jarrett, JT; Lansbury, PT, Jr. Amyloid fibril formation requires a chemically discriminating nucleation event: studies of an amyloidogenic sequence from the bacterial protein OsmB. Biochemistry 1992, 31, 12345–12352, doi:10.1021/bi00164a008. 1463722
- Shen, CL; Scott, GL; Merchant, F; Murphy, RM. Light scattering analysis of fibril growth from the amino-terminal fragment beta(1–28) of beta-amyloid peptide. Biophys. J 1993, 65, 2383–2395, doi:10.1016/S0006-3495(93)81312-2. 8312477
- Nielsen, L; Khurana, R; Coats, A; Frokjaer, S; Brange, J; Vyas, S; Uversky, VN; Fink, AL. Effect of environmental factors on the kinetics of insulin fibril formation: elucidation of the molecular mechanism. Biochemistry 2001, 40, 6036–6046, doi:10.1021/bi002555c. 11352739
- Fezoui, Y; Teplow, DB. Kinetic studies of amyloid beta-protein fibril assembly. Differential effects of alpha-helix stabilization. J. Biol. Chem 2002, 277, 36948–36954, doi:10.1074/jbc.M204168200. 12149256
- Kentsis, A; Borden, KL. Physical mechanisms and biological significance of supramolecular protein self-assembly. Curr. Protein Pept. Sci 2004, 5, 125–134, doi:10.2174/1389203043486856. 15078223
- Roberts, CJ. Non-native protein aggregation kinetics. Biotechnol. Bioeng 2007, 98, 927–938, doi:10.1002/bit.21627. 17705294
- Morris, AM; Watzky, MA; Finke, RG. Protein aggregation kinetics, mechanism, and curve-fitting: A review of the literature. Biochim. Biophys. Acta 2009, 1794, 375–397, doi:10.1016/j.bbapap.2008.10.016. 19071235
- Khurana, R; Gillespie, JR; Talapatra, A; Minert, LJ; Ionescu-Zanetti, C; Millett, I; Fink, AL. Partially folded intermediates as critical precursors of light chain amyloid fibrils and amorphous aggregates. Biochemistry 2001, 40, 3525–3535, doi:10.1021/bi001782b. 11297418
- Qin, Z; Hu, D; Zhu, M; Fink, AL. Structural characterization of the partially folded intermediates of an immunoglobulin light chain leading to amyloid fibrillation and amorphous aggregation. Biochemistry 2007, 46, 3521–3531, doi:10.1021/bi061716v. 17315948
- Kumar, S; Mohanty, SK; Udgaonkar, JB. Mechanism of formation of amyloid protofibrils of barstar from soluble oligomers: evidence for multiple steps and lateral association coupled to conformational conversion. J. Mol. Biol 2007, 367, 1186–11204, doi:10.1016/j.jmb.2007.01.039. 17292913
- Khodarahmi, R; Beyrami, M; Soori, H. Appraisal of casein's inhibitory effects on aggregation accompanying carbonic anhydrase refolding and heat-induced ovalbumin fibrillogenesis. Arch. Biochem. Biophys 2008, 477, 67–76, doi:10.1016/j.abb.2008.04.028. 18485276
- Andreasi Bassi, F; Arcovito, G; De Spirito, M; Mordente, A; Martorana, GE. Self-similarity properties of alpha-crystallin supramolecular aggregates. Biophys. J 1995, 69, 2720–2727, doi:10.1016/S0006-3495(95)80143-8. 8599678
- Bettelheim, FA; Ansari, R; Cheng, QF; Zigler, JS, Jr. The mode of chaperoning of dithiothreitol-denatured alpha-lactalbumin by alpha-crystallin. Biochem. Biophys. Res. Commun 1999, 261, 292–297, doi:10.1006/bbrc.1999.1031. 10425180
- Schuler, J; Frank, J; Saenger, W; Georgalis, Y. Thermally induced aggregation of human transferrin receptor studied by light-scattering techniques. Biophys. J 1999, 77, 1117–1125, doi:10.1016/S0006-3495(99)76962-6. 10423456
- Modler, AJ; Gast, K; Lutsch, G; Damaschun, G. Assembly of amyloid protofibrils via critical oligomers-a novel pathway of amyloid formation. J. Mol. Biol 2003, 325, 135–148, doi:10.1016/S0022-2836(02)01175-0. 12473457
- Baussay, K; Bon, CL; Nicolai, T; Durand, D; Busnel, JP. Influence of the ionic strength on the heat-induced aggregation of the globular protein beta-lactoglobulin at pH 7. Int. J. Biol. Macromol 2004, 34, 21–28, doi:10.1016/j.ijbiomac.2003.11.003. 15178005
- Follmer, C; Pereira, FV; Da Silveira, NP; Carlini, CR. Jack bean urease (EC 220.127.116.11) aggregation monitored by dynamic and static light scattering. Biophys. Chem 2004, 111, 79–87, doi:10.1016/j.bpc.2004.03.009. 15450378
- Militello, V; Casarino, C; Emanuele, A; Giostra, A; Pullara, F; Leone, M. Aggregation kinetics of bovine serum albumin studied by FTIR spectroscopy and light scattering. Biophys. Chem 2004, 107, 175–187, doi:10.1016/j.bpc.2003.09.004. 14962598
- Marchetti, S; Sbrana, F; Raccis, R; Lanzi, L; Gambi, CM; Vassalli, M; Tiribilli, B; Pacini, A; Toscano, A. Dynamic light scattering and atomic force microscopy imaging on fragments of beta-connectin from human cardiac muscle. Phys Rev E Stat Nonlin Soft Matter Phys 2008, 77, 021910:1–021910:11.
- Chu, B. Laser Light Scattering Basic Principles and Practice; Dover Publications, Inc: Mineola, New York, USA, 2007.
- Schärtl, W. Light Scattering from Polymer Solutions and Nanoparticle Dispersions; Springer-Verlag: Berlin, Germany, 2007.
- International standard ISO 22412:2008(E); Particle size analysis-Dynamic light scattering (DLS); International Organization for Standartization, 2008.
- Yudin, IK; Nikolaenko, GL; Kosov, VI; Agayan, VA; Anisimov, MA; Sengers, JV. Simple photon-correlation spectrometer for research and education. Int. J. Thermophys 1997, 18, 1237–1248, doi:10.1007/BF02575258.
- Markossian, KA; Khanova, HA; Kleimenov, SY; Levitsky, DI; Chebotareva, NA; Asryants, RA; Muronetz, VI; Saso, L; Yudin, IK; Kurganov, BI. Mechanism of thermal aggregation of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase. Biochemistry 2006, 45, 13375–13384, doi:10.1021/bi0610707. 17073459
- Khanova, HA; Markossian, KA; Kurganov, BI; Samoilov, AM; Kleimenov, SY; Levitsky, DI; Yudin, IK; Timofeeva, AC; Muranov, KO; Ostrovsky, MA. Mechanism of chaperone-like activity. Suppression of thermal aggregation of betaL-crystallin by alpha-crystallin. Biochemistry 2005, 44, 15480–15487, doi:10.1021/bi051175u. 16300396
- Panyukov, Y; Yudin, I; Drachev, V; Dobrov, E; Kurganov, B. The study of amorphous aggregation of tobacco mosaic virus coat protein by dynamic light scattering. Biophys. Chem 2007, 127, 9–18, doi:10.1016/j.bpc.2006.11.006. 17182167
- Meremyanin, AV; Eronina, TB; Chebotareva, NA; Kurganov, BI. Kinetics of thermal aggregation of glycogen phosphorylase b from rabbit skeletal muscle. Mechanism of protective action of alpha-crystallin. Biopolymers 2008, 89, 124–134, doi:10.1002/bip.20872. 17941008
- Weitz, DA; Huang, JS; Lin, MY; Sung, J. Limits of the fractal dimension for irreversible kinetic aggregation of gold colloids. Phys. Rev. Lett 1985, 54, 1416–1419, doi:10.1103/PhysRevLett.54.1416. 10031026
- Weitz, DA; Lin, MY. Dynamic scaling of cluster-mass distributions in kinetic colloid aggregation. Phys. Rev. Lett 1986, 57, 2037–2040, doi:10.1103/PhysRevLett.57.2037. 10033616
- Lin, MY; Lindsay, HM; Weitz, DA; Ball, RC; Klein, R; Meakin, P. Universality in colloid aggregation. Nature 1989, 939, 360–362.
- Lin, MY; Lindsay, HM; Weitz, DA; Ball, RC; Klein, R; Meakin, P. Universal reaction-limited colloid aggregation. Phys. Rev. A 1990, 41, 2005–2020, doi:10.1103/PhysRevA.41.2005. 9903311
- Lu, PJ; Zaccarelli, E; Ciulla, F; Schofield, AB; Sciortino, F; Weitz, DA. Gelation of particles with short-range attraction. Nature 2008, 453, 499–503, doi:10.1038/nature06931. 18497820
- Lin, MY; Lindsay, HM; Weitz, DA; Ball, RC; Klein, R; Meakin, P. Universality of fractal aggregates as probed by light scattering. Proc. R. Soc. Lond. A 1989, 423, 71–87, doi:10.1098/rspa.1989.0042.
- Creighton, TE. Proteins Structures and Molecular Properties; New York, USA, 1993.
- Keresztes-Nagy, S; Klapper, MH; Lazer, L; Klotz, IM. Hybridization experiments: evidence of dissociation equilibrium in hemerythrin. Science 1965, 150, 357–359, doi:10.1126/science.150.3694.357. 5833791
- Winklmair, D. A simple approach to the theory of cooperative aggregation of biological macromolecules. Arch. Biochem. Biophys 1971, 147, 509–514, doi:10.1016/0003-9861(71)90408-5. 5136099
- Chebotareva, NA; Meremyanin, AV; Makeeva, VF; Livanova, NB; Kurganov, BI. Cooperative self-association of phosphorylase kinase from rabbit skeletal muscle. Biophys. Chem 2008, 133, 45–53, doi:10.1016/j.bpc.2007.12.001. 18164537
- Berka, M; Rice, JA. Relation between aggregation kinetics and the structure of kaolinite aggregates. Langmuir 2005, 21, 1223–1229, doi:10.1021/la0478853. 15697264
- Golub, NV; Markossian, KA; Sholukh, MV; Muranov, KO; Kurganov, BI. Study of kinetics of thermal aggregation of mitochondrial aspartate aminotransferase by dynamic light scattering: protective effect of alpha-crystallin. Eur Biophys J 2009. doi:10.1007/s00249-009-0403-7.
- Golub, NV; Markossian, KA; Kasilovich, NV; Sholukh, MV; Orlov, VN; Kurganov, BI. Thermal inactivation, denaturation and aggregation of mitochondrial aspartate aminotransferase. Biophys. Chem 2008, 135, 125–131, doi:10.1016/j.bpc.2008.04.001. 18440694
- Eronina, TB; Chebotareva, NA; Bazhina, SG; Makeeva, VF; Kleymenov, SY; Kurganov, BI. Effect of proline on thermal inactivation, denaturation and aggregation of glycogen phosphorylase b from rabbit skeletal muscle. Biophys. Chem 2009, 141, 66–74, doi:10.1016/j.bpc.2008.12.007. 19155119
- Meremyanin, AV; Eronina, TB; Chebotareva, NA; Kleimenov, SY; Yudin, IK; Muranov, KO; Ostrovsky, MA; Kurganov, BI. Effect of alpha-crystallin on thermal aggregation of glycogen phosphorylase b from rabbit skeletal muscle. Biochemistry (Mosc) 2007, 72, 518–528, doi:10.1134/S0006297907050082.
- Golub, N; Meremyanin, A; Markossian, K; Eronina, T; Chebotareva, N; Asryants, R; Muronets, V; Kurganov, B. Evidence for the formation of start aggregates as an initial stage of protein aggregation. FEBS Lett 2007, 581, 4223–4227, doi:10.1016/j.febslet.2007.07.066. 17706198
- Sluzky, V; Tamada, JA; Klibanov, AM; Langer, R. Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces. Proc. Natl. Acad. Sci. USA 1991, 88, 9377–9381, doi:10.1073/pnas.88.21.9377. 1946348
- Pouzot, M; Nicolai, T; Durand, D; Benyahia, L. Structure factor and elasticity of a heat-set globular protein gel. Macromolecules 2004, 37, 614–620, doi:10.1021/ma035117x.
- Mehalebi, S; Nicolai, T; Durand, D. Light scattering study of heat-denatured globular protein aggregates. Int. J. Biol. Macromol 2008, 43, 129–135, doi:10.1016/j.ijbiomac.2008.04.002. 18485470
- de la Fuente, MA; Singh, H; Hemar, Y. Recent advances in the characterization of heat-induced aggregates and intermediates of whey proteins. Trends Food Sci. Technol 2002, 13, 262–274, doi:10.1016/S0924-2244(02)00133-4.
- Durand, D; Gimel, JC; Nicolai, T. Aggregation, gelation and phase separation of heat denatured globular proteins. Physica A 2002, 304, 253–265, doi:10.1016/S0378-4371(01)00514-3.
- Turoverov, KK; Biktashev, AG; Khaitlina, SY; Kuznetsova, IM. The structure and dynamics of partially folded actin. Biochemistry 1999, 38, 6261–6269, doi:10.1021/bi9900976. 10320355
- Ball, RC; Weitz, DA; Witten, TA; Leyvraz, F. Universal kinetics in reaction-limited aggregation. Phys. Rev. Lett 1987, 58, 274–277, doi:10.1103/PhysRevLett.58.274. 10034887
- Khanova, HA; Markossian, KA; Kleimenov, SY; Levitsky, DI; Chebotareva, NA; Golub, NV; Asryants, RA; Muronetz, VI; Saso, L; Yudin, IK; Muranov, KO; Ostrovsky, MA; Kurganov, BI. Effect of alpha-crystallin on thermal denaturation and aggregation of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase. Biophys. Chem 2007, 125, 521–531, doi:10.1016/j.bpc.2006.11.002. 17229514
- Rajaraman, K; Raman, B; Ramakrishna, T; Rao, CM. Interaction of human recombinant alphaA- and alphaB-crystallins with early and late unfolding intermediates of citrate synthase on its thermal denaturation. FEBS Lett 2001, 497, 118–123, doi:10.1016/S0014-5793(01)02451-6. 11377425
- Hook, DW; Harding, JJ. Molecular chaperones protect catalase against thermal stress. Eur. J. Biochem 1997, 247, 380–385, doi:10.1111/j.1432-1033.1997.00380.x. 9249050
- Marini, I; Moschini, R; Del Corso, A; Mura, U. Complete protection by alpha-crystallin of lens sorbitol dehydrogenase undergoing thermal stress. J. Biol. Chem 2000, 275, 32559–32565, doi:10.1074/jbc.M006133200. 10930418
- Hess, JF; FitzGerald, PG. Protection of a restriction enzyme from heat inactivation by [alpha]-crystallin. Mol. Vis 1998, 4, 29. 9873067
- Zhang, Y; Liu, X; Liu, J. Recombinant human alphaA-crystallin can protect the enzymatic activity of CpUDG against thermal inactivation. FEBS Lett 2005, 579, 2897–2900, doi:10.1016/j.febslet.2005.04.043. 15878551
- Markossian, KA; Golub, NV; Kleymenov, SY; Muranov, KO; Sholukh, MV; Kurganov, BI. Effect of α-crystallin on thermostability of mitochondrial aspartate aminotransferase. Int J Biol Macromol 2009. in press.
- Markossian, KA; Golub, NV; Khanova, HA; Levitsky, DI; Poliansky, NB; Muranov, KO; Kurganov, BI. Mechanism of thermal aggregation of yeast alcohol dehydrogenase I. Role of intramolecular chaperone. Biochim. Biophys. Acta 2008, 1784, 1286–1293, doi:10.1016/j.bbapap.2008.04.030. 18515108
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).