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Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan
Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal
Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan
Laboratory of Growth Regulation Chemistry, Akita Prefectural University, Akita 010-0195, Japan
Graduate School of Engineering, Hiroshima University (HU), Hiroshima, Japan
Research Center for Radiation Protection, National Institute of Radiological Sciences (NIRS), Chiba, Takoma Park, Maryland, USA
Department of Research Planning and Coordination, Japan NIOSH, Kawasaki, Japan
* Author to whom correspondence should be addressed.
Received: 5 February 2009; in revised form: 11 March 2009 / Accepted: 13 March 2009 / Published: 16 March 2009
Abstract: We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (g) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.
Keywords: Contaminated soil; γ-ray; Marker genes; Oryza sativa; Phytoalexins
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Rakwal, R.; Agrawal, G.K.; Shibato, J.; Imanaka, T.; Fukutani, S.; Tamogami, S.; Endo, S.; Sahoo, S.K.S.; Masuo, Y.; Kimura, S. Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model. Int. J. Mol. Sci. 2009, 10, 1215-1225.
Rakwal R, Agrawal GK, Shibato J, Imanaka T, Fukutani S, Tamogami S, Endo S, Sahoo SKS, Masuo Y, Kimura S. Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model. International Journal of Molecular Sciences. 2009; 10(3):1215-1225.
Rakwal, Randeep; Agrawal, Ganesh Kumar; Shibato, Junko; Imanaka, Tetsuji; Fukutani, Satoshi; Tamogami, Shigeru; Endo, Satoru; Sahoo, Sarata Kumar Sahoo; Masuo, Yoshinori; Kimura, Shinzo. 2009. "Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model." Int. J. Mol. Sci. 10, no. 3: 1215-1225.