Int. J. Mol. Sci. 2009, 10(3), 1215-1225; doi:10.3390/ijms10031215
Article

Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model

1 Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan 2 Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal 3 Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan 4 Laboratory of Growth Regulation Chemistry, Akita Prefectural University, Akita 010-0195, Japan 5 Graduate School of Engineering, Hiroshima University (HU), Hiroshima, Japan 6 Research Center for Radiation Protection, National Institute of Radiological Sciences (NIRS), Chiba, Takoma Park, Maryland, USA 7 Department of Research Planning and Coordination, Japan NIOSH, Kawasaki, Japan
* Author to whom correspondence should be addressed.
Received: 5 February 2009; in revised form: 11 March 2009 / Accepted: 13 March 2009 / Published: 16 March 2009
(This article belongs to the Special Issue Biotic and Abiotic Stress)
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Abstract: We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (g) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.
Keywords: Contaminated soil; γ-ray; Marker genes; Oryza sativa; Phytoalexins

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MDPI and ACS Style

Rakwal, R.; Agrawal, G.K.; Shibato, J.; Imanaka, T.; Fukutani, S.; Tamogami, S.; Endo, S.; Sahoo, S.K.S.; Masuo, Y.; Kimura, S. Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model. Int. J. Mol. Sci. 2009, 10, 1215-1225.

AMA Style

Rakwal R, Agrawal GK, Shibato J, Imanaka T, Fukutani S, Tamogami S, Endo S, Sahoo SKS, Masuo Y, Kimura S. Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model. International Journal of Molecular Sciences. 2009; 10(3):1215-1225.

Chicago/Turabian Style

Rakwal, Randeep; Agrawal, Ganesh Kumar; Shibato, Junko; Imanaka, Tetsuji; Fukutani, Satoshi; Tamogami, Shigeru; Endo, Satoru; Sahoo, Sarata Kumar Sahoo; Masuo, Yoshinori; Kimura, Shinzo. 2009. "Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model." Int. J. Mol. Sci. 10, no. 3: 1215-1225.

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