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Int. J. Mol. Sci. 2009, 10(3), 1215-1225; doi:10.3390/ijms10031215
Article

Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model

1, 2,* , 2
, 1
, 3
, 3
, 4
, 5
, 6
, 1
 and 7
1 Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), West, Tsukuba, Ibaraki 305-8569, Japan 2 Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal 3 Research Reactor Institute, Kyoto University (KURRI), Osaka, Japan 4 Laboratory of Growth Regulation Chemistry, Akita Prefectural University, Akita 010-0195, Japan 5 Graduate School of Engineering, Hiroshima University (HU), Hiroshima, Japan 6 Research Center for Radiation Protection, National Institute of Radiological Sciences (NIRS), Chiba, Takoma Park, Maryland, USA 7 Department of Research Planning and Coordination, Japan NIOSH, Kawasaki, Japan
* Author to whom correspondence should be addressed.
Received: 5 February 2009 / Revised: 11 March 2009 / Accepted: 13 March 2009 / Published: 16 March 2009
(This article belongs to the Special Issue Biotic and Abiotic Stress)
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Abstract

We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (g) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.
Keywords: Contaminated soil; γ-ray; Marker genes; Oryza sativa; Phytoalexins Contaminated soil; γ-ray; Marker genes; Oryza sativa; Phytoalexins
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Rakwal, R.; Agrawal, G.K.; Shibato, J.; Imanaka, T.; Fukutani, S.; Tamogami, S.; Endo, S.; Sahoo, S.K.S.; Masuo, Y.; Kimura, S. Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model. Int. J. Mol. Sci. 2009, 10, 1215-1225.

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