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Biomass Production Potential of a Wastewater Alga Chlorella vulgaris ARC 1 under Elevated Levels of CO2 and Temperature
Department of Biological and Agricultural Engineering, The University of Georgia, Athens, GA 30602, USA
Laboratory of Soil Microbiology, Division of Soil Science and Microbiology, Central Rice Research Institute, Cuttack 753006, Orissa, India
* Author to whom correspondence should be addressed.
Received: 1 January 2009; in revised form: 29 January 2009 / Accepted: 4 February 2009 / Published: 5 February 2009
Abstract: The growth response of Chlorella vulgaris was studied under varying concentrations of carbon dioxide (ranging from 0.036 to 20%) and temperature (30, 40 and 50oC). The highest chlorophyll concentration (11 µg mL-1) and biomass (210 µg mL-1), which were 60 and 20 times more than that of C. vulgaris at ambient CO2 (0.036%), were recorded at 6% CO2 level. At 16% CO2 level, the concentrations of chlorophyll and biomass values were comparable to those at ambient CO2 but further increases in the CO2 level decreased both of them. Results showed that the optimum temperature for biomass production was 30oC under elevated CO2 (6%). Although increases in temperature above 30oC resulted in concomitant decrease in growth response, their adverse effects were significantly subdued at elevated CO2. There were also differential responses of the alga, assessed in terms of NaH14CO3 uptake and carbonic anhydrase activity, to increases in temperature at elevated CO2. The results indicated that Chlorella vulgaris grew better at elevated CO2 level at 30oC, albeit with lesser efficiencies at higher temperatures.
Keywords: Biomass; carbonic anhydrase; Chlorella; CO2; 14C uptake; microalgae; temperature
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Chinnasamy, S.; Ramakrishnan, B.; Bhatnagar, A.; Das, K.C. Biomass Production Potential of a Wastewater Alga Chlorella vulgaris ARC 1 under Elevated Levels of CO2 and Temperature. Int. J. Mol. Sci. 2009, 10, 518-532.
Chinnasamy S, Ramakrishnan B, Bhatnagar A, Das KC. Biomass Production Potential of a Wastewater Alga Chlorella vulgaris ARC 1 under Elevated Levels of CO2 and Temperature. International Journal of Molecular Sciences. 2009; 10(2):518-532.
Chinnasamy, Senthil; Ramakrishnan, Balasubramanian; Bhatnagar, Ashish; Das, Keshav C. 2009. "Biomass Production Potential of a Wastewater Alga Chlorella vulgaris ARC 1 under Elevated Levels of CO2 and Temperature." Int. J. Mol. Sci. 10, no. 2: 518-532.