Metabolome and Transcriptome Sequencing Analysis Reveals Anthocyanin Metabolism in Pink Flowers of Anthocyanin-Rich Tea (Camellia sinensis)
Abstract
:1. Introduction
2. Results
2.1. Metabolomic Differences among Tea Flower Development Stages
2.2. Overview of Transcriptome Sequencing
2.3. Annotation and Identification of Unigenes
2.4. KEGG Pathway Analysis
2.5. Screening of Differentially Expressed Genes during Floral Development
2.6. Differential Expression Involved in Anthocyanins’ Biosynthetic Genes in Five Floral Development Stages
2.7. Gene Co-Expression and Crucial Gene Screening Using WGCNA
2.8. Validation of Structural Gene Expression qRT-PCR
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Samples Extraction and Targeted Metabolomics Analysis
4.2.1. Extraction of Secondary Metabolites
4.2.2. Liquid Chromatographic Mass Spectrometry Analysis
4.2.3. Metabolite Identification and Quantification
4.3. RNA Extraction, and Illumina Sequencing
4.4. Gene Functional Annotation and Expression Analysis
4.5. Gene screening by Co-Expression Analysis
4.6. Gene Validation and Expression Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Rothenberg, D.O.; Yang, H.; Chen, M.; Zhang, W.; Zhang, L. Metabolome and Transcriptome Sequencing Analysis Reveals Anthocyanin Metabolism in Pink Flowers of Anthocyanin-Rich Tea (Camellia sinensis). Molecules 2019, 24, 1064. https://doi.org/10.3390/molecules24061064
Rothenberg DO, Yang H, Chen M, Zhang W, Zhang L. Metabolome and Transcriptome Sequencing Analysis Reveals Anthocyanin Metabolism in Pink Flowers of Anthocyanin-Rich Tea (Camellia sinensis). Molecules. 2019; 24(6):1064. https://doi.org/10.3390/molecules24061064
Chicago/Turabian StyleRothenberg, Dylan O’Neill, Haijun Yang, Meiban Chen, Wenting Zhang, and Lingyun Zhang. 2019. "Metabolome and Transcriptome Sequencing Analysis Reveals Anthocyanin Metabolism in Pink Flowers of Anthocyanin-Rich Tea (Camellia sinensis)" Molecules 24, no. 6: 1064. https://doi.org/10.3390/molecules24061064