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Article

Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract

by
Katy Akoumany
1,2,
Agata Zykwinska
1,*,
Corinne Sinquin
1,
Laëtitia Marchand
1,
Mathieu Fanuel
3,
David Ropartz
3,
Hélène Rogniaux
3,
Muriel Pipelier
2,
Christine Delbarre-Ladrat
1 and
Sylvia Colliec-Jouault
1
1
Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, F-44311 Nantes, France
2
Université de Nantes, CNRS, Chimie et Interdisciplinarité: Synthèse, Analyse, Modélisation (CEISAM), UMR CNRS 6230, Faculté des Sciences et des Techniques, F-44322 Nantes, France
3
INRA, UR1268 Biopolymères Interactions Assemblages, F-44300 Nantes, France
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(19), 3441; https://doi.org/10.3390/molecules24193441
Submission received: 4 September 2019 / Revised: 18 September 2019 / Accepted: 19 September 2019 / Published: 22 September 2019
(This article belongs to the Special Issue Isolation of Marine Polysaccharides for Industrial and Biomedical Applications)
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Abstract

Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own EPS. In the present study, an enzymatic reaction was optimized to generate LMW derivatives of the GY785 EPS, which could advantageously replace the present bioactive derivatives obtained by a chemical process. Analysis by mass spectrometry of the oligosaccharide fractions released after enzymatic treatment revealed that mainly a lyase activity was responsible for the polysaccharide depolymerization. The repeating unit of the GY785 EPS produced by enzyme cleavage was then fully characterized.
Keywords: deep-sea bacterium; Alteromonas infernus; wild-type strain; exopolysaccharides; glycosaminoglycan-mimetic; enzymatic depolymerization; structural analysis; mass spectrometry deep-sea bacterium; Alteromonas infernus; wild-type strain; exopolysaccharides; glycosaminoglycan-mimetic; enzymatic depolymerization; structural analysis; mass spectrometry

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MDPI and ACS Style

Akoumany, K.; Zykwinska, A.; Sinquin, C.; Marchand, L.; Fanuel, M.; Ropartz, D.; Rogniaux, H.; Pipelier, M.; Delbarre-Ladrat, C.; Colliec-Jouault, S. Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract. Molecules 2019, 24, 3441. https://doi.org/10.3390/molecules24193441

AMA Style

Akoumany K, Zykwinska A, Sinquin C, Marchand L, Fanuel M, Ropartz D, Rogniaux H, Pipelier M, Delbarre-Ladrat C, Colliec-Jouault S. Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract. Molecules. 2019; 24(19):3441. https://doi.org/10.3390/molecules24193441

Chicago/Turabian Style

Akoumany, Katy, Agata Zykwinska, Corinne Sinquin, Laëtitia Marchand, Mathieu Fanuel, David Ropartz, Hélène Rogniaux, Muriel Pipelier, Christine Delbarre-Ladrat, and Sylvia Colliec-Jouault. 2019. "Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract" Molecules 24, no. 19: 3441. https://doi.org/10.3390/molecules24193441

APA Style

Akoumany, K., Zykwinska, A., Sinquin, C., Marchand, L., Fanuel, M., Ropartz, D., Rogniaux, H., Pipelier, M., Delbarre-Ladrat, C., & Colliec-Jouault, S. (2019). Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract. Molecules, 24(19), 3441. https://doi.org/10.3390/molecules24193441

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