Tyrosinase Inhibition Antioxidant Effect and Cytotoxicity Studies of the Extracts of Cudrania tricuspidata Fruit Standardized in Chlorogenic Acid

Round 1

Reviewer 1 Report

Chlorogenic acid is isolated from the leaves and fruits of dicotyledonous plants ¿because choosing a very common compound in plants as a biomarker? There may be another compound, specific to Cudrania tricuspidata fruit

Expand the introduction with the following publications on the subject

Lan-Ting Xin, Shi-Jun Yue, Ya-Chu Fan, Jing-Shuai Wu, Dan Yan, Hua-Shi Guan, Chang-Yun Wang (2017). Cudrania tricuspidata: an updated review on ethnomedicine, phytochemistry and pharmacology. RSC Adv., 7, 31807

Byung Woong Lee, Jin Hwan Lee, Sung-Tae Lee, Suh Awanchiri, Woo Song Lee, Tae-Sook Jeong, Ki Hun Park (2005). Antioxidant and cytotoxic activities of Xanthones from Cudrania tricuspidata. Bioorganic & Medicinal Chemistry Letters 15: 5548-5552

Jeong, CH., Choi, G.N., Kim, J.H. Kwak JH, Jeong HR, Kim DO, Heo HJ (2010). Protective effects of aqueous extract from Cudrania tricuspidata on oxidative stress-induced neurotoxicity. Food Sci Biotechnol 19: 1113.

Comments for author File: Comments.pdf

Author Response

Response to the Reviewer #1’s Comments

(molecules-588614)

Chlorogenic acid is isolated from the leaves and fruits of dicotyledonous plants because choosing a very common compound in plants as a biomarker There may be another compound, specific to Cudrania tricuspidata fruitExpand the introduction with the following publications on the subject

Authors’ Response: We deeply appreciate the reviewer’s positive evaluation and constructive comments on our manuscript. We thank the reviewer for introducing a reference pertinent our work, the sentence has been added in the instruction with additive references (line 38-39). the revised texts in the manuscript are highlighted with red color.

Minor correction

line 2 anti-oxidnat-> antioxidant

line 36 Cudramia tricuspidata -> C. tricuspidata

line 60 anti-oxidnat-> antioxidant

line87 anti-cancer-> anticancer

line138 ofC. tricuspidata-> of C. tricuspidata

line 157 asthe -> as the

line 175-176 100°C -> 100 °C

           ‒50°C -> ‒50 °C

line 219 2 hours -> 2h

line 214 1mL -> 1 mL

line230 C. tricuspidataas -> C. tricuspidata as

line232 ofchlorogenic acid in C. tricuspidatafruit -> of chlorogenic acid in C. tricuspidata fruit

line234 possessedthe -> possessed the

Author Response File: Author Response.docx

Reviewer 2 Report

Comments:

Please add different chromatograms from different real samples.  There many compounds contain similar structures with Chlorogenic Acid. How to confirm the selectivity and specificity of the proposed method? Authors only used the proposed method to detect one analyte, the run time 30 min is too long.  The LC conditions could be modified.

Author Response

Response to the Reviewer #2’s Comments

(molecules-588614)

Please add different chromatograms from different real samples.  There many compounds contain similar structures with Chlorogenic Acid. How to confirm the selectivity and specificity of the proposed method? Authors only used the proposed method to detect one analyte, the run time 30 min is too long.  The LC conditions could be modified.

Authors’ Response: We thank the reviewer for pointing this out. According to reviewers suggestion, we tried to add all the chromatograms of the assay samples to Figure 1, but the chromatogram was too large and complex.The reviewer pointed the analysis time (30min). In fact, we applied sufficient analysis time to identify rutin and 6,8-Diprenylgenistein reported in the references (including chlorogenic acid). However, we could not identified rutin and 6,8-Diprenylgenistein in the extract and described in the discussion section (line94-111). We ask the reviewer’s generous understanding on this matter.

Author Response File: Author Response.docx