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Molecules 2018, 23(9), 2232; https://doi.org/10.3390/molecules23092232

Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B

1
Department of Chemistry and Bioscience, Aalborg University, 9100 Aalborg, Denmark
2
Department of Chemistry and Bioscience, Aalborg University, 6700 Esbjerg, Denmark
*
Author to whom correspondence should be addressed.
Received: 12 July 2018 / Revised: 28 August 2018 / Accepted: 1 September 2018 / Published: 2 September 2018
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B. View Full-Text
Keywords: gibepyrones; prolipyrone; secondary metabolites; polyketide synthases; Fusarium gibepyrones; prolipyrone; secondary metabolites; polyketide synthases; Fusarium
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Westphal, K.R.; Muurmann, A.T.; Paulsen, I.E.; Nørgaard, K.T.H.; Overgaard, M.L.; Dall, S.M.; Aalborg, T.; Wimmer, R.; Sørensen, J.L.; Sondergaard, T.E. Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B. Molecules 2018, 23, 2232.

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