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Molecules 2018, 23(9), 2214; https://doi.org/10.3390/molecules23092214

Chemical Fingerprinting and Quantification of Chinese Cinnamomi Cortex by Ultra High Performance Liquid Chromatography Coupled with Chemometrics Methods

1,†
,
2,†
,
1,2,* , 2,* , 1
and
2
1
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2
Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 19 July 2018 / Revised: 29 August 2018 / Accepted: 30 August 2018 / Published: 31 August 2018
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Abstract

To rapidly clarify and quantify the chemical profiling of Cinnamomi cortex a reliable and feasible strategy of chromatographic fingerprinting with a suite of chemometrics methods was developed and validated by ultra-high performance liquid chromatography coupled with diode array detection. Furthermore, to identify more meaningful chemical markers, the chemometrics methods including hierarchical cluster analysis (HCA), principal component analysis (PCA) and similarity, which all generate quality evaluations and correlation classifications of Cinnamomi cortex, were used to improve the Cinnamomi cortex quality control standards. A total of 12 characteristic peaks were confirmed, seven of which were identified by comparing their retention times, UV and MS spectra with authentic compounds. Moreover, 11 analytes were accurately determined, as a complementary quantification method of chromatographic fingerprinting. For quantitative analyses, selective detection was performed at 254, 280 and 340 nm. The tested samples were separated and determined using UPLC and a series of methodologies including linearity, precision, accuracy, limit of detection and quantification and extraction recoveries were validated. Meanwhile the method bias for all the analytes did not exceed 5%. A total of 42 samples were acquired in China and analyzed. The results demonstrated that chromatographic fingerprinting in combination with chemometrics methods provides a promising and practical method to more effectively and comprehensively control the quality of Cinnamomi cortex from various sources, which would be a useful reference for the development and further study of Cinnamomi cortex and related formulations. View Full-Text
Keywords: ultra-high performance liquid chromatography; Cinnamomi cortex; quantification; Principal component analysis; hierarchical clustering analysis ultra-high performance liquid chromatography; Cinnamomi cortex; quantification; Principal component analysis; hierarchical clustering analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ma, N.; Ding, Y.; Zhang, Y.; Zhang, T.; Yi, Y.; Wang, B. Chemical Fingerprinting and Quantification of Chinese Cinnamomi Cortex by Ultra High Performance Liquid Chromatography Coupled with Chemometrics Methods. Molecules 2018, 23, 2214.

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