Next Article in Journal
Synthesis and Promotion of the Osteoblast Proliferation Effect of Morroniside Derivatives
Previous Article in Journal
Gold-Based Medicine: A Paradigm Shift in Anti-Cancer Therapy?
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(6), 1411; https://doi.org/10.3390/molecules23061411

Polyphenols from Tamarix nilotica: LC–ESI-MSn Profiling and In Vivo Antifibrotic Activity

1
Department of Pharmacognosy, Faculty of Pharmacy, British University in Egypt (BUE), Cairo 11837, Egypt
2
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
3
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
4
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
5
Laboratory of Applied Analytical and Environmental Chemistry, Department of Chemistry, Humboldt-Universität zu Berlin, 10099 Berlin, Germany
*
Author to whom correspondence should be addressed.
Received: 9 May 2018 / Revised: 30 May 2018 / Accepted: 3 June 2018 / Published: 11 June 2018
Full-Text   |   PDF [6197 KB, uploaded 11 June 2018]   |  

Abstract

Tamarix nilotica (Ehrenb.) Bunge (Tamaricaceae), an indigenous plant to the Middle East region, is well-known as a medicinal plant for treating many human ailments. The current study aimed at exploring the polyphenol profile of the alcohol soluble fraction of aqueous T. nilotica extract, assessing its in vivo antifibrotic activity and the possible underlying mechanism, to unravel the impact of quantitative difference of sulphated polyphenols content on the antifibrotic activity of T. nilotca grown in two different habitats. Polyphenol profiling of T. nilotica extracts was performed using HPLC-HRESI-QTOF-MS-MS. The major polyphenol components included sulphated flavonoids, phenolic acids and free aglycones. The antifibrotic activity was evaluated through carbon tetrachloride-induced liver fibrosis in rats. Biochemical evaluations revealed that both fractions ameliorated the increased levels of hepatic aminotransferases, lipid peroxidation, hydroxyproline, α-smooth muscle actin (α-SMA), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-κB). Moreover, both fractions reduced catalase activity (CAT) and enhanced hepatic glutathione (GSH) content. Histopathological imaging undoubtedly confirmed such results. In conclusion, the T. nilotica polyphenol-rich fraction exhibited potential antifibrotic activity in rats. Significant alterations in GSH levels were recorded based on the sulphated polyphenol metabolite content. View Full-Text
Keywords: Tamarix nilotica; Tamaricaceae; polyphenols; HPLC/HRESI/MS/MS; antifibrotic activity Tamarix nilotica; Tamaricaceae; polyphenols; HPLC/HRESI/MS/MS; antifibrotic activity
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Sekkien, A.; Swilam, N.; Ebada, S.S.; Esmat, A.; El-Khatib, A.H.; Linscheid, M.W.; Singab, A.N. Polyphenols from Tamarix nilotica: LC–ESI-MSn Profiling and In Vivo Antifibrotic Activity. Molecules 2018, 23, 1411.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top