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Molecules 2018, 23(6), 1287; https://doi.org/10.3390/molecules23061287

The Growth Proliferation, Apoptotic Prevention, and Differentiation Induction of the Gelatin Hydrolysates from Three Sources to Human Fetal Osteoblasts (hFOB 1.19 Cells)

1
and
1,2,*
1
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China
2
Department of Food Science, Northeast Agricultural University, Harbin 150030, China
*
Author to whom correspondence should be addressed.
Academic Editor: Silvia Panzavolta
Received: 1 April 2018 / Revised: 26 April 2018 / Accepted: 8 May 2018 / Published: 28 May 2018
(This article belongs to the Special Issue Gelatin: Chemistry, Characterization, Application)
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Abstract

Gelatins from the skin of bovine, porcine, and tilapia were hydrolyzed to three degrees of hydrolysis (DH) by alcalase, neutrase, and papain, respectively. These hydrolysates at 0.02–0.1 g/L promoted the growth of human fetal osteoblasts by 101.4–135.7%, while higher DH or using papain and tilapia gelatins resulted in higher proliferation. The hydrolysates from porcine and tilapia gelatins at 0.05 g/L prevented induced apoptosis (decreasing total apoptotic proportions from 28.4% or 35.2% to 10.3–17.5% or 16.0–23.6%), and had differentiation induction (increasing alkaline phosphatase activity by 126.9–246.7% in early differentiation stage, or enhancing osteocalcin production by 4.1–22.5% in later differentiation stage). These hydrolysates had a similar amino acid profile; however, tilapia gelatin hydrolysates by papain with DH 15.4% mostly displayed higher activity than others. Tilapia gelatin hydrolysate could up-regulate β-catenin, Wnt 3a, Wnt 10b, cyclin D1, and c-Myc expression at mRNA levels by 1.11–3.60 folds, but down-regulate GSK 3β expression by 0.98 fold. Of note, β-catenin in total cellular and nuclear protein was up-regulated by 1.14–1.16 folds but unchanged in cytoplasmic protein, Wnt 10b, cyclin D1, and c-Myc expression were up-regulated by 1.27–1.95 folds, whilst GSK 3β expression was down-regulated by 0.87 fold. Activation of Wnt/β-catenin pathway is suggested to mediate cell proliferation and differentiation. View Full-Text
Keywords: gelatin hydrolysate; human fetal osteoblasts; proliferation; apoptosis; differentiation; Wnt/β-catenin pathway gelatin hydrolysate; human fetal osteoblasts; proliferation; apoptosis; differentiation; Wnt/β-catenin pathway
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Lu, M.; Zhao, X.-H. The Growth Proliferation, Apoptotic Prevention, and Differentiation Induction of the Gelatin Hydrolysates from Three Sources to Human Fetal Osteoblasts (hFOB 1.19 Cells). Molecules 2018, 23, 1287.

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