Effects of UV-B Radiation on the Content of Bioactive Components and the Antioxidant Activity of Prunella vulgaris L. Spica during Development
Abstract
:1. Introduction
2. Results
2.1. Effects of UV-B Radiation on the Contents of Total Flavonoids, Rosmarinic Acid and Caffeic Acid
2.2. Effects of UV-B Radiation on the Contents of Salviaflaside and Hyperoside
2.3. Effects of UV-B Radiation on the Antioxidant Activities
2.4. Correlation Analysis
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. UV-B Treatments
4.3. Determination of Total Flavonoids Content
4.4. Determination of the Caffeic Acid, Salviaflaside, Rosmarinic Acid and Hyperoside Contents
4.5. DPPH Free Radical Scavenging Assay
4.6. Trolox Equivalent Antioxidant Capacity Assay (TEAC Assay)
4.7. Statistical Analyses
5. Conclusions
Supplementary Materials
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the total flavonoids, rosmarinic acid, caffeic acid, salviaflaside and hyperoside are available from the authors. |
Antioxidant Index | UV-B Dosage (μW cm−2 nm−1) | Bud Stage | Full-Flowering | Mature-Fruiting |
---|---|---|---|---|
DPPH● (%) | 0 | 90.41 ± 0.23 aA | 91.24 ± 0.10 aA | 74.36 ± 2.90 aB |
120 | 90.35 ± 0.83 aA | 88.61 ± 0.34 bA | 70.49 ± 2.62 aB | |
TEAC (mmol L−1 Trolox) | 0 | 1.31 ± 0.01 aA | 1.35 ± 0.02 aA | 0.80 ± 0.05 bB |
120 | 1.13 ± 0.05 bB | 1.33 ± 0.05 aA | 1.00 ± 0.02 aC |
Antioxidant Index | UV-B Dose (μW cm−2 nm−1) | Rosmarinic Acid (%) | Caffeic Acid (%) | Hyperoside (%) | Salviaflaside (%) | Total Flavonoids (%) |
---|---|---|---|---|---|---|
DPPH● (%) | 0 | 0.993 * | 0.727 | 0.967 | −0.999 * | 0.999 * |
120 | 0.187 | 0.189 | 0.229 | 0.312 | 0.999 * | |
TEAC (mmol L−1 Trolox) | 0 | 0.191 | 0.202 | 0.244 | 0.334 | 0.999 * |
120 | 0.132 | 0.136 | 0.163 | 0.224 | 0.720 |
Standard Chemicals | Regression Equation | R2 | Linear Range |
---|---|---|---|
Total flavonoids | y = 0.5899x + 0.0199 | 0.9999 | 0.00–2.00 |
Rosmarinic acid | y = 39999x + 0.0031 | 0.9999 | 0.000209–0.001045 |
Caffeic acid | y = 49130x − 0.0422 | 0.9999 | 0.000108–0.00054 |
Salviaflaside | y = 23282x − 0.044 | 0.9999 | 0.00022–0.0011 |
Hyperoside | y = 33699x − 0.0094 | 0.9999 | 0.00011–0.00055 |
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Chen, Y.; Zhang, X.; Guo, Q.; Liu, L.; Li, C.; Cao, L.; Qin, Q.; Zhao, M.; Wang, W. Effects of UV-B Radiation on the Content of Bioactive Components and the Antioxidant Activity of Prunella vulgaris L. Spica during Development. Molecules 2018, 23, 989. https://doi.org/10.3390/molecules23050989
Chen Y, Zhang X, Guo Q, Liu L, Li C, Cao L, Qin Q, Zhao M, Wang W. Effects of UV-B Radiation on the Content of Bioactive Components and the Antioxidant Activity of Prunella vulgaris L. Spica during Development. Molecules. 2018; 23(5):989. https://doi.org/10.3390/molecules23050989
Chicago/Turabian StyleChen, Yuhang, Xuerong Zhang, Qiaosheng Guo, Li Liu, Chen Li, Liping Cao, Qin Qin, Miao Zhao, and Wenming Wang. 2018. "Effects of UV-B Radiation on the Content of Bioactive Components and the Antioxidant Activity of Prunella vulgaris L. Spica during Development" Molecules 23, no. 5: 989. https://doi.org/10.3390/molecules23050989