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Molecules 2018, 23(5), 1157; https://doi.org/10.3390/molecules23051157

A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae

1,2,†
,
1,3,†
,
1,3
,
2,4
,
2,4
and
1,2,4,*
1
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
2
State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen 518083, China
3
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China
4
Key Lab of Genomics, Chinese Ministry of Agriculture, BGI-Shenzhen, Shenzhen 518083, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 25 April 2018 / Revised: 9 May 2018 / Accepted: 10 May 2018 / Published: 11 May 2018
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Abstract

Bile salt hydrolase (BSH) is a well-known enzyme that has been commonly characterized in probiotic bacteria, as it has cholesterol-lowering effects. However, its molecular investigations are scarce. Here, we build a local database of BSH sequences from Lactobacillaceae (BSH–SDL), and phylogenetic analysis and homology searches were employed to elucidate their comparability and distinctiveness among species. Evolutionary study demonstrates that BSH sequences in BSH–SDL are divided into five groups, named BSH A, B, C, D and E here, which can be the genetic basis for BSH classification and nomenclature. Sequence analysis suggests the differences between BSH-active and BSH-inactive proteins clearly, especially on site 82. In addition, a total of 551 BSHs from 107 species are identified from 451 genomes of 158 Lactobacillaceae species. Interestingly, those bacteria carrying various copies of BSH A or B can be predicted to be potential cholesterol-lowering probiotics, based on the results of phylogenetic analysis and the subtypes that those previously reported BSH-active probiotics possess. In summary, this study elaborates the molecular basis of BSH in Lactobacillaceae systematically, and provides a novel methodology as well as a consistent standard for the identification of the BSH subtype. We believe that high-throughput screening can be efficiently applied to the selection of promising candidate BSH-active probiotics, which will advance the development of healthcare products in cholesterol metabolism. View Full-Text
Keywords: bile salt hydrolase (BSH); Lactobacillaceae; genome survey; BSH classification; cholesterol-lowering probiotics bile salt hydrolase (BSH); Lactobacillaceae; genome survey; BSH classification; cholesterol-lowering probiotics
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Liang, L.; Yi, Y.; Lv, Y.; Qian, J.; Lei, X.; Zhang, G. A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae. Molecules 2018, 23, 1157.

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