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Molecules 2018, 23(5), 1018; https://doi.org/10.3390/molecules23051018

A Chemical Investigation of the Leaves of Morus alba L.

1
Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, USA
2
Institute of Medical Information & Library, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100020, China
3
Beijing Key Laboratory of Bioactive Substances and Function Foods, Beijing Union University, Beijing 100191, China
4
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
*
Author to whom correspondence should be addressed.
Received: 31 March 2018 / Revised: 21 April 2018 / Accepted: 24 April 2018 / Published: 26 April 2018
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Abstract

The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves of Morus alba L. resulted in four new structures 14 and a known molecule 5. Compounds 2 and 5 inhibited aldose reductase with IC50 values of 4.33 μM and 6.0 μM compared with the potent AR inhibitor epalrestat (IC50 1.88 × 10−3 μM). Pretreatment with compound 3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001). View Full-Text
Keywords: Morus alba L.; aldose reductase inhibitor; neuroprotective agent; natural products Morus alba L.; aldose reductase inhibitor; neuroprotective agent; natural products
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chen, X.-Y.; Zhang, T.; Wang, X.; Hamann, M.T.; Kang, J.; Yu, D.-Q.; Chen, R.-Y. A Chemical Investigation of the Leaves of Morus alba L.. Molecules 2018, 23, 1018.

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