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Molecules 2018, 23(3), 656; https://doi.org/10.3390/molecules23030656

Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix

1
Department of Pharmacognosy, College of Pharmaceutical Sciences, Matsuyama University, 4-2 Bunkyo-cho, Matsuyama, Ehime 790-8578, Japan
2
Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan
*
Author to whom correspondence should be addressed.
Received: 5 February 2018 / Revised: 5 March 2018 / Accepted: 12 March 2018 / Published: 14 March 2018
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Abstract

A new phenolic compound, 2-O-β-laminaribiosyl-4-hydroxyacetophenone (1), was isolated from Cynanchi Wilfordii Radix (CWR, the root of Cynanchum wilfordii Hemsley), along with 10 known aromatic compounds, including cynandione A (2), bungeisides-C (7) and –D (8), p-hydroxyacetophenone (9), 2′,5′-dihydroxyacetophenone (10), and 2′,4′-dihydroxyacetophenone (11). The structure of the new compound (1) was elucidated using spectroscopic methods and chemical methods. The structure of cynandione A (2), including a linkage mode of the biphenyl parts that remained uncertain, was unambiguously confirmed using the 2D 13C–13C incredible natural abundance double quantum transfer experiment (INADEQUATE) spectrum. Additionally, health issues related to the use of Cynanchi Auriculati Radix (CAR, the root of Cynanchum auriculatum Royle ex Wight) instead of CWR have emerged. Therefore, constituents present in methanolic extracts of commercially available CWRs and CARs were examined using UV-sensitive high-performance liquid chromatography (HPLC), resulting in common detection of three major peaks ascribed to cynandione A (2), p-hydroxyacetophenone (9), and 2′,4′-dihydroxyacetophenone (11). Thus, to distinguish between these ingredients, a thin-layer chromatography (TLC) method, combined with only UV irradiation detection, focusing on wilfosides C1N (12) and K1N (13) as marker compounds characteristic of CAR, was performed. Furthermore, we propose this method as a simple and convenient strategy for the preliminary distinction of CWR and CAR to ensure the quality and safety of their crude drugs. View Full-Text
Keywords: Cynanchum wilfordii; phenolic glycoside; 2-O-β-laminaribiosyl-4-hydroxyacetophenone; cynandione A; thin layer chromatography; Cynanchum auriculatum Cynanchum wilfordii; phenolic glycoside; 2-O-β-laminaribiosyl-4-hydroxyacetophenone; cynandione A; thin layer chromatography; Cynanchum auriculatum
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Uchikura, T.; Tanaka, H.; Sugiwaki, H.; Yoshimura, M.; Sato-Masumoto, N.; Tsujimoto, T.; Uchiyama, N.; Hakamatsuka, T.; Amakura, Y. Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix. Molecules 2018, 23, 656.

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