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Molecules 2018, 23(2), 188; doi:10.3390/molecules23020188

Jasmonate-Elicited Stress Induces Metabolic Change in the Leaves of Leucaena leucocephala

1
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
4
Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
*
Author to whom correspondence should be addressed.
Received: 30 December 2017 / Revised: 13 January 2018 / Accepted: 16 January 2018 / Published: 24 January 2018
(This article belongs to the Section Metabolites)
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Abstract

The plant Leucaena leucocephala was exposed to four jasmonate elicitors, i.e., jasmonic acid (JA), methyl jasmonic acid (MeJA), jasmonoyl-l-isoleucine (JA-Ile) and 6-ethyl indanoyl glycine conjugate (2-[(6-ethyl-1-oxo-indane-4-carbonyl)-amino]-acetic acid methyl ester) (CGM). The treatment was to mimic the herbivores and wounding stresses. By using NMR spectroscopy along with chemometric analysis, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), the changes of metabolites in the leaves of L. leucocephala were determined under the stress as induced by the four elicitors. The challenge of JA-Ile caused an accumulation of lactic acid (6), β-glucose (10), alanine (12), threonine (13), steroids (18), 3,4-dihydroxypyridine (19) and an unidentified compound 20. The chemometric analysis of the PCA and PLS-DA models indicated that the alternation of metabolites triggered by JA, MeJA, and CGM treatments were very minimum. In contrast, the treatment by JA-Ile could induce the most significant metabolic changes in the leaves. Moreover, there was very minimal new metabolite being detected in responding to the jasmonate-induced stresses. The results showed some metabolite concentrations changed after application of the elicitors, which may be related to a high level of tolerance to stress conditions as well as the strong ecological suitability of L. leucocephala. View Full-Text
Keywords: Leucaena leucocephala; NMR; principal component analysis; partial least squares discriminant analysis; elicitors; environmental stress Leucaena leucocephala; NMR; principal component analysis; partial least squares discriminant analysis; elicitors; environmental stress
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MDPI and ACS Style

Xu, Y.; Tao, Z.; Jin, Y.; Chen, S.; Zhou, Z.; Gong, A.G.W.; Yuan, Y.; Dong, T.T.X.; Tsim, K.W.K. Jasmonate-Elicited Stress Induces Metabolic Change in the Leaves of Leucaena leucocephala. Molecules 2018, 23, 188.

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