Aquaporin 11-Dependent Inhibition of Proliferation by Deuterium Oxide in Activated Hepatic Stellate Cells
Abstract
:1. Introduction
2. Results
2.1. Activation of the p53-Cyclin-Dependent Pathway by D2O Leads to Cell Cycle Arrest
2.2. HSC Proliferation Is AQP11 Dependent
2.3. Inhibition of HO-1 Activity Increases the Antifibrotic Effect of D2O
2.4. Excess Accumulation of ATP Diminishes Cell Proliferation
3. Discussion
4. Materials and Methods
4.1. Cell and Tissue Cultures
4.2. Reagents and Chemicals
4.3. Cell Proliferation
4.4. An Apoptosis Assay
4.5. Western Blotting and Antibodies
4.6. The Intracellular ATP/ADP Ratio
4.7. Analysis of the Cell Cycle
4.8. Intracellular ROS Levels
4.9. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Lee, P.J.; Park, H.-J.; Cho, N.; Kim, H.P. Aquaporin 11-Dependent Inhibition of Proliferation by Deuterium Oxide in Activated Hepatic Stellate Cells. Molecules 2018, 23, 3209. https://doi.org/10.3390/molecules23123209
Lee PJ, Park H-J, Cho N, Kim HP. Aquaporin 11-Dependent Inhibition of Proliferation by Deuterium Oxide in Activated Hepatic Stellate Cells. Molecules. 2018; 23(12):3209. https://doi.org/10.3390/molecules23123209
Chicago/Turabian StyleLee, Phil Jun, Hye-Jin Park, Namki Cho, and Hong Pyo Kim. 2018. "Aquaporin 11-Dependent Inhibition of Proliferation by Deuterium Oxide in Activated Hepatic Stellate Cells" Molecules 23, no. 12: 3209. https://doi.org/10.3390/molecules23123209