Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora
AbstractIxeris dentata var. albiflora is considered as a potential therapeutic agent against mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors as well as good seasoned vegetable in Far East countries. Phytoene synthase (PSY), phytoene desaturase (PDS) ξ-carotene desaturase (ZDS), lycopene β-cyclase (LCYB), lycopene ε-cyclase (LCYE), ε-ring carotene hydroxylase (CHXB), and zeaxanthin epoxidase (ZDS) are vital enzymes in the carotenoid biosynthesis pathway. We have examined these seven genes from I. dentata that are participated in carotenoid biosynthesis utilizing an Illumina/Solexa HiSeq 2000 platform. In silico analysis of the seven deduced amino acid sequences were revealed its closest homology with other Asteracea plants. Further, we explored transcript levels and carotenoid accumulation in various organs of I. dentata using quantitative real time PCR and high-performance liquid chromatography, respectively. The highest transcript levels were noticed in the leaf for all the genes while minimal levels were noticed in the root. The maximal carotenoid accumulation was also detected in the leaf. We proposed that these genes expressions are associated with the accumulation of carotenoids. Our findings may suggest the fundamental clues to unravel the molecular insights of carotenoid biosynthesis in various organs of I. dentata. View Full-Text
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Reddy, C.S.; Lee, S.-H.; Yoon, J.S.; Kim, J.K.; Lee, S.W.; Hur, M.; Koo, S.C.; Meilan, J.; Lee, W.M.; Jang, J.K.; Hur, Y.; Park, S.U.; Kim, Y.B. Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora. Molecules 2017, 22, 1449.
Reddy CS, Lee S-H, Yoon JS, Kim JK, Lee SW, Hur M, Koo SC, Meilan J, Lee WM, Jang JK, Hur Y, Park SU, Kim YB. Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora. Molecules. 2017; 22(9):1449.Chicago/Turabian Style
Reddy, Chinreddy Subramanyam; Lee, Sang-Hoon; Yoon, Jeong Su; Kim, Jae Kwang; Lee, Sang Won; Hur, Mok; Koo, Sung Cheol; Meilan, Jin; Lee, Woo Moon; Jang, Jae Ki; Hur, Yoonkang; Park, Sang Un; Kim, and Yeon Bok. 2017. "Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora." Molecules 22, no. 9: 1449.
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