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Molecules 2017, 22(9), 1449; doi:10.3390/molecules22091449

Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora

1
Department of Herbal Crop Resources, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun 27709, Korea
2
Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Korea
3
Department of Biology, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
4
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
5
Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea
This authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 14 August 2017 / Revised: 26 August 2017 / Accepted: 30 August 2017 / Published: 31 August 2017
(This article belongs to the Section Natural Products)
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Abstract

Ixeris dentata var. albiflora is considered as a potential therapeutic agent against mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors as well as good seasoned vegetable in Far East countries. Phytoene synthase (PSY), phytoene desaturase (PDS) ξ-carotene desaturase (ZDS), lycopene β-cyclase (LCYB), lycopene ε-cyclase (LCYE), ε-ring carotene hydroxylase (CHXB), and zeaxanthin epoxidase (ZDS) are vital enzymes in the carotenoid biosynthesis pathway. We have examined these seven genes from I. dentata that are participated in carotenoid biosynthesis utilizing an Illumina/Solexa HiSeq 2000 platform. In silico analysis of the seven deduced amino acid sequences were revealed its closest homology with other Asteracea plants. Further, we explored transcript levels and carotenoid accumulation in various organs of I. dentata using quantitative real time PCR and high-performance liquid chromatography, respectively. The highest transcript levels were noticed in the leaf for all the genes while minimal levels were noticed in the root. The maximal carotenoid accumulation was also detected in the leaf. We proposed that these genes expressions are associated with the accumulation of carotenoids. Our findings may suggest the fundamental clues to unravel the molecular insights of carotenoid biosynthesis in various organs of I. dentata. View Full-Text
Keywords: Ixeris dentata var. albiflora; carotenoid; transcript level Ixeris dentata var. albiflora; carotenoid; transcript level
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Reddy, C.S.; Lee, S.-H.; Yoon, J.S.; Kim, J.K.; Lee, S.W.; Hur, M.; Koo, S.C.; Meilan, J.; Lee, W.M.; Jang, J.K.; Hur, Y.; Park, S.U.; Kim, Y.B. Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora. Molecules 2017, 22, 1449.

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