Multienzyme Biosynthesis of Dihydroartemisinic Acid
AbstractOne-pot multienzyme biosynthesis is an attractive method for producing complex, chiral bioactive compounds. It is advantageous over step-by-step synthesis, as it simplifies the process, reduces costs and often leads to higher yield due to the synergistic effects of enzymatic reactions. In this study, dihydroartemisinic acid (DHAA) pathway enzymes were overexpressed in Saccharomyces cerevisiae, and whole-cell biotransformation of amorpha-4,11-diene (AD) to DHAA was demonstrated. The first oxidation step by cytochrome P450 (CYP71AV1) is the main rate-limiting step, and a series of N-terminal truncation and transcriptional tuning improved the enzymatic activity. With the co-expression of artemisinic aldehyde dehydrogenase (ALDH1), which recycles NADPH, a significant 8-fold enhancement of DHAA production was observed. Subsequently, abiotic conditions were optimized to further enhance the productivity of the whole-cell biocatalysts. Collectively, approximately 230 mg/L DHAA was produced by the multi-step whole-cell reaction, a ~50% conversion from AD. This study illustrates the feasibility of producing bioactive compounds by in vitro one-pot multienzyme reactions. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 838 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Chen, X.; Zhang, C.; Too, H.-P. Multienzyme Biosynthesis of Dihydroartemisinic Acid. Molecules 2017, 22, 1422.
Chen X, Zhang C, Too H-P. Multienzyme Biosynthesis of Dihydroartemisinic Acid. Molecules. 2017; 22(9):1422.Chicago/Turabian Style
Chen, Xixian; Zhang, Congqiang; Too, Heng-Phon. 2017. "Multienzyme Biosynthesis of Dihydroartemisinic Acid." Molecules 22, no. 9: 1422.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.