Bioactive Constituents Obtained from the Seeds of Lepidium apetalum Willd
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental
3.1. General
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Evaluation of Effects on Sodium Oleate-Induced TG Overloading in HepG2 Cells
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of all compounds are available from the authors. |
No. | δC | δH (J in Hz) | No. | δC | δH (J in Hz) |
---|---|---|---|---|---|
2 | 156.7 | — | 2′′′ | 73.0 | 3.28 (dd, 7.0, 9.5) |
3 | 133.4 | — | 3′′′ | 76.2 | 3.32 (dd, 9.5, 9.5) |
4 | 177.6 | — | 4′′′ | 69.2 | 3.26 (m, overlapped) |
5 | 160.8 | — | 5′′′ | 75.3 | 3.75 (m) |
6 | 99.4 | 6.51 (br. s) | 6′′′ | 68.9 | 3.71 (dd, 5.5, 11.5) |
7 | 162.7 | — | 3.99 (br. d, ca. 12) | ||
8 | 94.4 | 6.85 (br. s) | 1′′′′ | 103.5 | 4.35 (d, 8.0) |
9 | 155.9 | — | 2′′′′ | 71.5 | 3.22 (dd, 7.5, 8.0) |
10 | 105.6 | — | 3′′′′ | 77.5 | 4.90 (dd, 7.5, 9.0) |
1′ | 120.7 | — | 4′′′′ | 68.1 | 3.34 (dd, 9.0, 9.0) |
2′,6′ | 130.9 | 8.09 (d, 9.0) | 5′′′′ | 77.4 | 3.08 (m) |
3′,5′ | 115.2 | 6.92 (d, 9.0) | 6′′′′ | 60.7 | 3.56 (br. d, ca. 12) |
4′ | 160.1 | — | 3.70 (dd, 5.5, 11.5) | ||
5-OH | — | 12.65 (br. s) | 1′′′′′ | 124.5 | — |
1′′ | 100.6 | 5.50 (d, 8.0) | 2′′′′′,6′′′′′ | 105.9 | 7.00 (s) |
2′′ | 74.2 | 3.21 (dd, 7.5, 8.0) | 3′′′′′,5′′′′′ | 147.9 | — |
3′′ | 76.8 | 3.26 (m, overlapped) | 4′′′′′ | 138.0 | — |
4′′ | 69.8 | 3.08 (m, overlapped) | 7′′′′′ | 144.9 | 7.51 (d, 16.0) |
5′′ | 76.3 | 3.21 (m) | 8′′′′′ | 115.5 | 6.53 (d, 16.0) |
6′′ | 60.8 | 3.30 (br. d, ca. 11) | 9′′′′′ | 166.2 | — |
3.50 (dd, 5.5, 10.5) | 3′′′′′,5′′′′′-OCH3 | 56.0 | 3.81 (s) | ||
1′′′ | 99.7 | 5.12 (d, 7.0) |
No. | δC | δH (J in Hz) | No. | δC | δH (J in Hz) |
---|---|---|---|---|---|
1 | 130.2 | — | 4′ | 70.0 a | 3.07 (m, overlapped) |
2,6 | 105.6 | 6.58 (s) | 5′ | 78.1 | 3.12 (m, overlapped) |
3,5 | 147.5 | — | 6′ | 61.2 b | 3.46 (dd, 5.0, 12.5) |
4 | 134.5 | — | 3.70 (br. d, ca. 13) | ||
7 | 46.5 | 4.28 (br. d, ca. 5) | 1′′ | 84.7 | 4.27 (d, 10.0) |
8 | 34.8 | 3.18 (br. d, ca. 11) | 2′′ | 80.8 | 3.13 (m, overlapped) |
3.39 (dd, 5.0, 11.0) | 3′′ | 78.0 | 3.12 (m, overlapped) | ||
3,5-OCH3 | 55.9 | 3.75 (s) | 4′′ | 69.9 a | 3.07 (m, overlapped) |
1′ | 83.7 | 4.31 (d, 9.5) | 5′′ | 72.9 | 3.01 (dd, 9.5, 10.0) |
2′ | 72.9 | 3.01 (dd, 8.0, 9.5) | 6′′ | 61.1 b | 3.46 (dd, 5.0, 12.5) |
3′ | 80.8 | 3.13 (m, overlapped) | 3.70 (br. d, ca. 13) |
No. | δC | δH (J in Hz) | No. | δC | δH (J in Hz) |
---|---|---|---|---|---|
1 | 96.1 | 5.57 (d, 5.5) | 1′ | 92.4 | 4.68 (d, 9.0) |
2 | 74.3 | 3.87 (dd, 5.5, 9.5) | 2′ | 74.4 | 3.49 (dd, 9.0, 9.5) |
3 | 76.2 | 3.58 (dd, 9.5, 9.5) | 3′ | 80.0 | 3.52 (dd, 9.5, 9.5) |
4 | 72.3 | 3.44 (dd, 9.5, 9.5) | 4′ | 72.1 | 3.42 (dd, 9.5, 9.5) |
5 | 76.4 | 3.94 (m) | 5′ | 83.1 | 3.50 (m) |
6 | 63.4 | 3.80 (dd, 5.5, 12.5) | 6′ | 63.7 | 3.72 (dd, 5.5, 12.5) |
3.88 (dd, 1.5, 12.5) | 3.91 (dd, 1.5, 12.5) |
Sample (µmol/L) | 7 | 8 | 17 | ||||||
---|---|---|---|---|---|---|---|---|---|
30 | 3 | 0.3 | 30 | 3 | 0.3 | 30 | 3 | 0.3 | |
TG clearance (%) | 9.46 ± 1.89 | 7.16 ± 0.87 | 3.24 ± 0.35 | 12.00 ± 1.17 | 8.27 ± 2.14 | 4.15 ± 1.97 | 12.70 ± 2.39 | 5.68 ± 1.15 | 4.32 ± 1.57 |
Sample (µmol/L) | 20 | 25 | ||||||
---|---|---|---|---|---|---|---|---|
100 | 30 | 3 | 0.3 | 100 | 30 | 3 | 0.3 | |
TG clearance (%) | 4.14 ± 1.40 | 3.91 ± 1.79 | 2.82 ± 1.06 | 1.31 ± 1.02 | 12.66 ± 0.77 | 3.91 ± 0.90 | 2.96 ± 0.45 | 2.00 ± 0.34 |
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Wang, S.; Shi, P.; Qu, L.; Ruan, J.; Yang, S.; Yu, H.; Zhang, Y.; Wang, T. Bioactive Constituents Obtained from the Seeds of Lepidium apetalum Willd. Molecules 2017, 22, 540. https://doi.org/10.3390/molecules22040540
Wang S, Shi P, Qu L, Ruan J, Yang S, Yu H, Zhang Y, Wang T. Bioactive Constituents Obtained from the Seeds of Lepidium apetalum Willd. Molecules. 2017; 22(4):540. https://doi.org/10.3390/molecules22040540
Chicago/Turabian StyleWang, Sijian, Pingping Shi, Lu Qu, Jingya Ruan, Shengcai Yang, Haiyang Yu, Yi Zhang, and Tao Wang. 2017. "Bioactive Constituents Obtained from the Seeds of Lepidium apetalum Willd" Molecules 22, no. 4: 540. https://doi.org/10.3390/molecules22040540