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Molecules 2017, 22(4), 538; doi:10.3390/molecules22040538

Determination of Vancomycin in Human Serum by Cyclodextrin-Micellar Electrokinetic Capillary Chromatography (CD-MEKC) and Application for PDAP Patients

1
Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, No. 100 Haining Road, Shanghai 200080, China
2
Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3
Laboratory of Drug Metabolism & Pharmacokinetics, School of Medicine, Tongji University, No. 1239 Siping Road, Shanghai 200092, China
4
Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, No. 24 Tong Jia Xiang, Nanjing 210009, China
5
Department of Pharmacy, Shanghai Fourth People's Hospital, Shanghai 200081, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 21 February 2017 / Revised: 18 March 2017 / Accepted: 22 March 2017 / Published: 28 March 2017
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Abstract

A simple and sensitive cyclodextrin-micellar electrokinetic capillary chromatography (CD-MEKC) method with UV detection was developed and validated for the determination of vancomycin (VCM) in serum. The separation was achieved in 14 min at 25 °C with a fused-silica capillary column of 40.2 cm × 75 μm i.d. (effective length 30.2 cm) and a run buffer containing 25 mM borate buffer with 50 mM sodium dodecylsulfonate (SDS) (pH 9.5) and 2% sulfobutyl-β-cyclodextrin (sulfobutyl-β-CD). Under optimal conditions for biological samples, good separations with high efficiency and short analysis time were achieved. Several parameters affecting the drug separation from biological matrices were studied, including buffer types, concentrations, and pHs. The methods were validated over the range of 0.9998–99.98 µg/mL. Calibration curves of VCM also showed good linearity (r2 > 0.999). Intra- and interday precisions (relative standard deviation, RSD) were less than 5.80% and 7.38%, and lower limit of quantification (LLOQ) were lower than 1.0 μg/mL. The mean recoveries ranged between 84.03% and 91.69%. The method was successfully applied for monitoring VCM concentrations in serum of patients with peritoneal dialysis-associated peritonitis (PDAP). The assay should be applicable to pharmacokinetic studies and routine therapeutic drug monitoring of this drug in serum. View Full-Text
Keywords: vancomycin; cyclodextrin-micellar electrokinetic capillary chromatography; peritoneal dialysis-associated peritonitis vancomycin; cyclodextrin-micellar electrokinetic capillary chromatography; peritoneal dialysis-associated peritonitis
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Wang, J.; Cao, Y.; Wu, S.; Wang, S.; Zhao, X.; Zhou, T.; Lou, Y.; Fan, G. Determination of Vancomycin in Human Serum by Cyclodextrin-Micellar Electrokinetic Capillary Chromatography (CD-MEKC) and Application for PDAP Patients. Molecules 2017, 22, 538.

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