DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity
AbstractThe development of a switching system for guanine nanowire (G-wire) formation by external signals is important for nanobiotechnological applications. Here, we demonstrate a DNA nanostructural switch (G-wire <--> particles) using a designed peptide and a protease. The peptide consists of a PNA sequence for inducing DNA to form DNA–PNA hybrid G-quadruplex structures, and a protease substrate sequence acting as a switching module that is dependent on the activity of a particular protease. Micro-scale analyses via TEM and AFM showed that G-rich DNA alone forms G-wires in the presence of Ca2+, and that the peptide disrupted this formation, resulting in the formation of particles. The addition of the protease and digestion of the peptide regenerated the G-wires. Macro-scale analyses by DLS, zeta potential, CD, and gel filtration were in agreement with the microscopic observations. These results imply that the secondary structure change (DNA G-quadruplex <--> DNA/PNA hybrid structure) induces a change in the well-formed nanostructure (G-wire <--> particles). Our findings demonstrate a control system for forming DNA G-wire structures dependent on protease activity using designed peptides. Such systems hold promise for regulating the formation of nanowire for various applications, including electronic circuits for use in nanobiotechnologies. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 4881 KB)
Share & Cite This Article
Usui, K.; Okada, A.; Sakashita, S.; Shimooka, M.; Tsuruoka, T.; Nakano, S.-I.; Miyoshi, D.; Mashima, T.; Katahira, M.; Hamada, Y. DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity. Molecules 2017, 22, 1991.
Usui K, Okada A, Sakashita S, Shimooka M, Tsuruoka T, Nakano S-I, Miyoshi D, Mashima T, Katahira M, Hamada Y. DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity. Molecules. 2017; 22(11):1991.Chicago/Turabian Style
Usui, Kenji; Okada, Arisa; Sakashita, Shungo; Shimooka, Masayuki; Tsuruoka, Takaaki; Nakano, Shu-ichi; Miyoshi, Daisuke; Mashima, Tsukasa; Katahira, Masato; Hamada, Yoshio. 2017. "DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity." Molecules 22, no. 11: 1991.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.