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Molecules 2016, 21(6), 768; doi:10.3390/molecules21060768

Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins

Department of Pharmacognosy, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China
Department of Pharmacy, the Second Part of the First Hospital, Jilin University, Changchun 130021, China
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 7 May 2016 / Revised: 5 June 2016 / Accepted: 8 June 2016 / Published: 13 June 2016
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The use of macroporous resins for the separation and purification of total flavonoids to obtain high-purity total flavonoids from Scorzonera austriaca was studied. The optimal conditions for separation and purification of total flavonoids in S. austriaca with macroporous resins were as follows: D4020 resin columns were loaded with crude flavonoid extract solution, and after reaching adsorptive saturation, the columns were eluted successively with 5 bed volumes (BV) of water, 5 BV of 5% (v/v) aqueous ethanol and 5 BV of 30% (v/v) aqueous ethanol at an elute flow rate of 2 BV·h−1. Total flavonoids were obtained from the 30% aqueous ethanol eluate by vacuum distillation recovery. The content of flavonoid compounds in the total flavonoids was 93.5%, which represents an improvement by about 150%. In addition, five flavonoid compounds in the product were identified as 2″-O-β-d-xylopyranosyl isoorientin, 6-C-α-l-arabipyranosyl orientin, orientin, isoorientin and vitexin by LC-ESI-MS analysis and internal standard methods. The results in this study could represent a method for the large-scale production of total flavonoids from S. austriaca. View Full-Text
Keywords: Scorzonera austriaca; flavonoids; macroporous resins Scorzonera austriaca; flavonoids; macroporous resins

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Xie, Y.; Guo, Q.-S.; Wang, G.-S. Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins. Molecules 2016, 21, 768.

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