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Molecules 2016, 21(6), 701; doi:10.3390/molecules21060701

Purification, Characterization and Biological Activity of Polysaccharides from Dendrobium officinale

1
College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2
The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510080, China
3
Department of Anatomy, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Christopher W. K. Lam
Received: 22 March 2016 / Revised: 24 May 2016 / Accepted: 25 May 2016 / Published: 30 May 2016
(This article belongs to the Collection Herbal Medicine Research)
View Full-Text   |   Download PDF [4910 KB, uploaded 30 May 2016]   |  

Abstract

Polysaccharide (DOPA) from the stem of D. officinale, as well as two fractions (DOPA-1 and DOPA-2) of it, were isolated and purified by DEAE cellulose-52 and Sephacryl S-300 chromatography, and their structural characteristics and bioactivities were investigated. The average molecular weights of DOPA-1 and DOPA-2 were 394 kDa and 362 kDa, respectively. They were mainly composed of d-mannose, d-glucose, and had a backbone consisting of 1,4-linked β-d-Manp and 1,4-linked β-d-Glcp with O-acetyl groups. Bioactivity studies indicated that both DOPA and its purified fractions (DOPA-1 and DOPA-2) could activate splenocytes and macrophages. The D. officinale polysaccharides had stimulatory effects on splenocytes, T-lymphocytes and B-lymphocytes, promoting the cell viability and NO production of RAW 264.7 macrophages. Furthermore, DOPA, DOPA-1 and DOPA-2 were found to protect RAW 264.7 macrophages against hydrogen peroxide (H2O2)-induced oxidative injury by promoting cell viability, suppressing apoptosis and ameliorating oxidative lesions. These results suggested that D. officinale polysaccharides possessed antioxidant activity and mild immunostimulatory activity. View Full-Text
Keywords: Dendrobium officinale; polysaccharide; structural characterization; immunostimulatory activity; antioxidant activity Dendrobium officinale; polysaccharide; structural characterization; immunostimulatory activity; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Huang, K.; Li, Y.; Tao, S.; Wei, G.; Huang, Y.; Chen, D.; Wu, C. Purification, Characterization and Biological Activity of Polysaccharides from Dendrobium officinale. Molecules 2016, 21, 701.

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