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Molecules 2016, 21(3), 319; doi:10.3390/molecules21030319

Differential Response of Two Human Breast Cancer Cell Lines to the Phenolic Extract from Flaxseed Oil

CROM, Istituto Nazionale Tumori “Fondazione G. Pascale”-IRCCS, Napoli 80131, Italy
Istituto di Scienze dell'Alimentazione, CNR, Avellino 83100, Italy
Istituto di Biologia Agro-ambientale e Forestale, CNR, UOS di Napoli, Napoli 80131, Italy
Direttore Scientifico, Istituto Nazionale Tumori “Fondazione G. Pascale”-IRCCS, Napoli 80131, Italy
Servizio di Informatica Medica, Azienda Ospedaliera Universitaria, Seconda Università di Napoli, Napoli 80138, Italy
Dedicated to the birth of Mattia Evangelista with wishes for a long and peaceful life.
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 30 January 2016 / Revised: 26 February 2016 / Accepted: 2 March 2016 / Published: 8 March 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1376 KB, uploaded 8 March 2016]   |  


Many studies have evidenced that the phenolic components from flaxseed (FS) oil have potential health benefits. The effect of the phenolic extract from FS oil has been evaluated on two human breast cancer cell lines, MCF7 and MDA-MB231, and on the human non-cancerous breast cell line, MCF10A, by SRB assay, cellular death, cell cycle, cell signaling, lipid peroxidation and expression of some key genes. We have evidenced that the extract shows anti-proliferative activity on MCF7 cells by inducing cellular apoptosis, increase of the percentage of cells in G0/G1 phase and of lipid peroxidation, activation of the H2AX signaling pathway, and upregulation of a six gene signature. On the other hand, on the MDA-MB2131 cells we verified only an anti-proliferative activity, a weak lipid peroxidation, the activation of the PI3K signaling pathway and an up-regulation of four genes. Overall these data suggest that the extract has both cytotoxic and pro-oxidant effects only on MCF7 cells, and can act as a metabolic probe, inducing differences in the gene expression. For this purpose, we have performed an interactomic analysis, highlighting the existing associations. From this approach, we show that the phenotypic difference between the two cell lines can be explained through their differential response to the phenolic extract. View Full-Text
Keywords: flaxseed; phenolic extract; breast cancer cell lines; gene regulation flaxseed; phenolic extract; breast cancer cell lines; gene regulation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sorice, A.; Guerriero, E.; Volpe, M.G.; Capone, F.; La Cara, F.; Ciliberto, G.; Colonna, G.; Costantini, S. Differential Response of Two Human Breast Cancer Cell Lines to the Phenolic Extract from Flaxseed Oil. Molecules 2016, 21, 319.

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