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Molecules 2016, 21(1), 37; doi:10.3390/molecules21010037

Peptide KSL-W-Loaded Mucoadhesive Liquid Crystalline Vehicle as an Alternative Treatment for Multispecies Oral Biofilm

1
School of Pharmaceutical Sciences, Sao Paulo State University, UNESP, Rodovia Araraquara-Jaú Km 01, Araraquara, SP 14800-850, Brazil
2
Chemistry Institute, Sao Paulo State University, UNESP, Campus Araraquara, Araraquara, SP 14800-900, Brazil
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexandru Mihai Grumezescu
Received: 30 October 2015 / Revised: 4 December 2015 / Accepted: 4 December 2015 / Published: 25 December 2015
(This article belongs to the Special Issue Pharmaceutical Nanotechnology: Novel Approaches)
View Full-Text   |   Download PDF [1953 KB, uploaded 25 December 2015]   |  

Abstract

Decapeptide KSL-W shows antibacterial activities and can be used in the oral cavity, however, it is easily degraded in aqueous solution and eliminated. Therefore, we aimed to develop liquid crystalline systems (F1 and F2) for KSL-W buccal administration to treat multispecies oral biofilms. The systems were prepared with oleic acid, polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20), and a 1% poloxamer 407 dispersion as the oil phase (OP), surfactant (S), and aqueous phase (AP), respectively. We characterized them using polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, and in vitro bioadhesion, and performed in vitro biological analysis. PLM showed isotropy (F1) or anisotropy with lamellar mesophases (F2), confirmed by peak ratio quantification using SAXS. Rheological tests demonstrated that F1 exhibited Newtonian behavior but not F2, which showed a structured AP concentration-dependent system. Bioadhesion studies revealed an AP concentration-dependent increase in the system’s bioadhesiveness (F2 = 15.50 ± 1.00 mN·s) to bovine teeth blocks. Antimicrobial testing revealed 100% inhibition of multispecies oral biofilm growth after KSL-W administration, which was incorporated in the F2 aqueous phase at a concentration of 1 mg/mL. Our results suggest that this system could serve as a potential vehicle for buccal administration of antibiofilm peptides. View Full-Text
Keywords: liquid crystalline systems; oral cavity; biofilm; KSL-W; antimicrobial peptide liquid crystalline systems; oral cavity; biofilm; KSL-W; antimicrobial peptide
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Bernegossi, J.; Calixto, G.M.F.; Sanches, P.R.S.; Fontana, C.R.; Cilli, E.M.; Garrido, S.S.; Chorilli, M. Peptide KSL-W-Loaded Mucoadhesive Liquid Crystalline Vehicle as an Alternative Treatment for Multispecies Oral Biofilm. Molecules 2016, 21, 37.

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