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Molecules 2016, 21(1), 2; doi:10.3390/molecules21010002

Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon echinus (Nees & Mart.) Schauer

1
Centro de Ciências Biológicas e da Saúde-CCBS, Departamento de Ciências Biológicas, Universidade Regional do Cariri (URCA), Pimenta, Crato CEP 63.100-000, CE, Brazil
2
Programa de Pós-Graduação em Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil
3
Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil
4
Laboratório de Botânica Aplicada, Departamento de Ciências Biológicas, Universidade Regional do Cariri (URCA), Pimenta, Crato CEP 63.100-000, CE, Brazil
5
Laboratório de Farmacologia e Química Molecular, Departamento de Química Biológica, Universidade Regional do Cariri, Pimenta, Crato CEP 63.100-000, CE, Brazil
6
Laboratório de Pesquisas de Produtos Naturais, Departamento de Química Biológica, Universidade Regional do Cariri, Crato CEP 63.105.000, CE, Brazil
7
Laboratório de Fitoquímica, Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil
8
Functional Foods and Nutraceutical Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria
9
Departamento de Bioquímica, Instituto de Ciências Básica da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre CEP 90035-003, RS, Brazil
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 25 October 2015 / Revised: 8 December 2015 / Accepted: 9 December 2015 / Published: 29 December 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [2378 KB, uploaded 29 December 2015]   |  

Abstract

Raphiodon echinus (R. echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. echinus on human erythrocytes and leukocytes were investigated for the first time. R. echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. echinus exhibited antioxidant activity in DPPH radical scavenging with IC50 = 111.9 μg/mL (EtOH extract) and IC50 = 227.9 μg/mL (aqueous extract). The extracts inhibited Fe2+ (10 μM) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30–480 μg/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine. View Full-Text
Keywords: Raphiodon echinus; antioxidant activity; phenolic acids; HPLC-DAD Raphiodon echinus; antioxidant activity; phenolic acids; HPLC-DAD
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Duarte, A.E.; Waczuk, E.P.; Roversi, K.; da Silva, M.A.P.; Barros, L.M.; da Cunha, F.A.B.; de Menezes, I.R.A.; da Costa, J.G.M.; Boligon, A.A.; Ademiluyi, A.O.; Kamdem, J.P.; Rocha, J.B.T.; Burger, M.E. Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon echinus (Nees & Mart.) Schauer. Molecules 2016, 21, 2.

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