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Molecules 2016, 21(1), 118; doi:10.3390/molecules21010118

A Rapid Screening Analysis of Antioxidant Compounds in Native Australian Food Plants Using Multiplexed Detection with Active Flow Technology Columns

1
Food Science and Technology Research Group, School of Science and Health, Western Sydney University (Hawkesbury), Hawkesbury 1797, Australia
2
Australian Centre for Research on Separation Sciences (ACROSS), School of Science and Health, Western Sydney University (Parramatta), Room LZ.G.73, Corner of Pemberton Street and Victoria Road, Parramatta 2150, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Jean-Marc Sabatier
Received: 18 November 2015 / Revised: 11 January 2016 / Accepted: 11 January 2016 / Published: 20 January 2016
(This article belongs to the Section Natural Products)
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Abstract

Conventional techniques for identifying antioxidant and phenolic compounds in native Australian food plants are laborious and time-consuming. Here, we present a multiplexed detection technique that reduces analysis time without compromising separation performance. This technique is achieved using Active Flow Technology-Parallel Segmented Flow (AFT-PSF) columns. Extracts from cinnamon myrtle (Backhousia myrtifolia) and lemon myrtle (Backhousia citriodora) leaves were analysed via multiplexed detection using an AFT-PSF column with underivatised UV-VIS, mass spectroscopy (MS), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) derivatisation for antioxidants as detection methods. A number of antioxidant compounds were detected in the extracts of each leaf extract. View Full-Text
Keywords: multiplexed detection; high performance liquid chromatography (HPLC); active flow technology (AFT); post-column derivatisation (PCD); antioxidants; lemon myrtle; cinnamon myrtle; native Australian food plants multiplexed detection; high performance liquid chromatography (HPLC); active flow technology (AFT); post-column derivatisation (PCD); antioxidants; lemon myrtle; cinnamon myrtle; native Australian food plants
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Rupesinghe, E.J.R.; Jones, A.; Shalliker, R.A.; Pravadali-Cekic, S. A Rapid Screening Analysis of Antioxidant Compounds in Native Australian Food Plants Using Multiplexed Detection with Active Flow Technology Columns. Molecules 2016, 21, 118.

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